IR-4 Ornamental Horticulture Program Research Report Cover Sheet

Transcription

1 e.pdf IR-4 Ornamental Horticulture Program Research Report Cover Sheet Researcher(s) Nora J Catlin Trial: 2015 NY Affiliation: Cornell Cooperative Extension of Suffol 423 Griffing Avenue Riverhead NY PhoneNumber: x2 njc23@cornell.edu ProjectTitle: Botrytis Efficacy Protocol #: PR# Research Target Crop/Plant Product EPA Reg. # Production Site Status Botrytis elliptica Botrytis elliptica Lily Lilium sp. Emblem (aka NUP 09092) fludioxonil Nufarm Field Container C Botrytis elliptica Botrytis elliptica Lily Lilium sp. F9110 F FMC Field Container C Botrytis elliptica Botrytis elliptica Lily Lilium sp. MBI 110 MBI110 - Marrone Bio In Field Container C Botrytis elliptica Botrytis elliptica Lily Lilium sp. Mural (A18126B) WDG Azoxystrobin + benzovindiflu Syngenta Field In-Ground C Botrytis elliptica Botrytis elliptica Lily Lilium sp. Orkestra Intrinsic (BAS703 0 Fluxapyroxad + pyraclostrobi BASF Field Container C Botrytis elliptica Botrytis elliptica Lily Lilium sp. Proud 3 Thyme oil (5.6%) - Biohumanetics Field Container C Botrytis elliptica Botrytis elliptica Lily Lilium sp. S2200 4SC Mandestrobin - Valent Field Container C

2 Ornamental Horticulture Program Research Report Form p 1 Researcher: Nora Catlin Date: 11/10/2016 Project Title: Efficacy of Management Tools for Botrytis Protocol #: PRnumbers: 32345, 32346, 32675, 32676, 32679, 32347, Narrative Summary (Results/Discussion) Eight products were tested for their efficacy in managing Botrytis blight of lily, caused by Botrytis eliptica. Tested products included: fluxapryoxad+pyraclostrobin (BAS70306F; 8 oz/100 gal), Bacillus amyloliquifaciens strain F727 (MBI110; 1 gal/100 gal), fludioxonil (NUP 09092; 4 oz/100gal and 8 oz/100gal), thyme oil (Proud 3; 1 gal/100 gal), mandestrobin (S2200; 7.5 oz/100 gal and 15 oz/100 gal), benzovindiflupyr+azoxystrobin (Mural; 7 oz/100 gal), fenhexamid (Decree; 1.5 lb/100 gal), and a protein extracted from Lupinus spp. (20%; F9110; 24 oz/100 gal). Products were applied weekly or bi weekly. An untreated uninoculated control and an untreated inoculated control were also included. Treatments were replicated across 8 single plant replicates per treatment. Plants were maintained in the greenhouse until 1 day after the first treatment after which they were moved into a hoop house fitted with shade cloth and overhead irrigation in order to provide ideal disease conditions. Containers of symptomatic plants were placed among the plants to serve as inoculation and the untreated uninoculated control plants were set on the opposite side of the house, in an un shaded section a distance away from the inoculum. Plants were evaluated weekly, starting one week after treatment. Disease severity was evaluated using a 0 10 scale (0=no symptoms), the number of affected leaves was recorded, and initial and final height and width measurements were also recorded. Observations on phytotoxicity were also taken. The trial plants were maintained in conditions that were ideal for disease development: shady conditions with overhead irrigation. Despite these ideal conditions, no to little disease was observed for the first 4 weeks. In order to help encourage disease development, the irrigation timing was shifted from a 10 minute morning and a 10 minute midafternoon irrigation, to a 10 minute morning and 10 minute early evening irrigation to provide a longer period of leaf wetness. After this change, disease symptoms developed to a satisfactory level for the last three weeks of the trial. On 20 Aug, a few significant differences were found in the number of affected leaves between treatments, but no differences were found in the disease severity ratings (Table 1). On the 25 Aug and 1 Sept evaluations, significant differences were found between treatments in the number of affected leaves as well as disease severity (Tables 1 2). No differences in height and width were found (Table 3). Fluxapryoxad+pyraclostrobin (BAS70306F), fludioxonil (NUP 09092, both rates), mandestrobin (S2200, both rates), benzovindiflupyr+azoxystrobin (Mural), and the untreated uninoculated treatments had significantly fewer affected leaves and significantly lower severity ratings than the Bacillus amyloliquifaciens (MBI110), thyme oil (Proud 3), fenhexamid (Decree), F9110, and the untreated inoculated treatments. Similar to a trial conducted in 2014 (Efficacy of Management Tools for Botrytis, Protocol ), fenhexamid (Decree) treatments resulted in intermediate to poor management of Botyrtis, which is surprising since this is considered a standard product and good option for managing Botrytis cinerea in greenhouse ornamentals. Plants were in bloom at the time of the 13 Aug treatment applications. On the 14 Aug evaluation, some phytotoxicity on the flower petals was observed on most replicates treated with the high rate of mandestrobin (S2200), and a few replicates treated with benzoviniflupyr + azoxystrobin (Mural) (Table 4). Symptoms were bleached, necrotic spots and patches. Since blooms are short lived, these symptoms were only observed during the 14 Aug evaluation. No other symptoms of phytotoxitity were observed through out the trail. These products should not be recommended for lilies in bloom.

3 Ornamental Horticulture Program Research Report Form p 2 Researcher: Nora Catlin Date: 11/10/2016 Project Title: Efficacy of Management Tools for Botrytis Protocol #: PRnumbers: 32345, 32346, 32675, 32676, 32679, 32347, Results Table Table 1. Average number of leaves showing symptoms on lily Dazzle plants treated with various management products and untreated plants. Treatment Fluxapryoxad + pyraclostrobin (BAS703 06F) Protein extracted from Lupinus sp., 20% (F9110) Bacillus amyloliquifaciens (MBI110) Rate/ 100 gal Number of Leaves with Symptoms 16 July 21 July 27 July 4 Aug 14 Aug 20 Aug 25 Aug 1 Sept 8 oz a 0.3 a 0.5 ab 0.9 c 2.9 b 24 oz a 0.5 a 0.5 ab 34.9 ab 61.1 a 1 gal a 0.8 a 2.0 ab 42.0 ab 61.0 a Fludioxonil (NUP09092) 4 oz a 0.4 a 0.6 ab 4.8 c 18.5 b Fudioxonil (NUP09092) 8 oz a 0.3 a 0.5 ab 1.6 c 7.0 b Thyme oil, 5.6% (Proud 3) 1 gal a 1.0 a 3.5 a 37.1 ab 61.0 a Mandestrobin (S2200) 7.5 oz a 0.1 a 0.4 ab 1.9 c 22.3 b Mandestrobin (S2200) 15 oz a 0 a 0.5 ab 1.1 c 8.0 b Benzoviniflupyr + azoxystrobin (Mural) 7 oz a 0.3 a 0.3 b 2.9 c 11.4 b Untreated uninoculated a 0 a 0.3 b 1.3 c 7.5 b Untreated inoculated a 0.1 a 2.5 ab 45.3 a 60.9 a Fenhexamid (Decree) 1.5 lb a 0.1 a 1.1 ab 29.6 b 64.0 a Means within a column with similar letters are not significantly different according to Tukey s HSD (p=0.05)

4 Ornamental Horticulture Program Research Report Form p 3 Researcher: Nora Catlin Date: 11/10/2016 Project Title: Efficacy of Management Tools for Botrytis Protocol #: PRnumbers: 32345, 32346, 32675, 32676, 32679, 32347, Figure 1. Average number of leaves showing symptoms on lily Dazzle plants treated with various management products and untreated plants. 70 Lilies Number of Leaves Affected Leaves /16 7/21 7/27 8/4 8/14 8/20 8/25 9/1 Date Fluxapryoxad + pyraclostrobin (BAS703 06F) F9110 Bacillus amyloliquifaciens (MBI110) fludioxonil (NUP09092) fludioxonil (NUP09092) Thyme oil, 5.6% (Proud 3) Mandestrobin (S2200) Mandestrobin (S2200) Benzoviniflupyr + azoxystrobin (Mural) Untreated uninoculated Untreated inoculated Fenhexamid (Decree)

5 Ornamental Horticulture Program Research Report Form p 4 Researcher: Nora Catlin Date: 11/10/2016 Project Title: Efficacy of Management Tools for Botrytis Protocol #: PRnumbers: 32345, 32346, 32675, 32676, 32679, 32347, Table 2. Average disease severity rating for lily Dazzle plants treated with various management products and untreated plants. Treatment Fluxapryoxad + pyraclostrobin (BAS703 06F) Protein extracted from Lupinus sp., 20% (F9110) Bacillus amyloliquifaciens (MBI110) Rate/ 100 gal Disease Severity Rating 16 July 21 July 27 July 4 Aug 14 Aug 20 Aug 25 Aug 1 Sept 8 oz a 0 a 0.1 a 0.3 a 0.5 c 1.0 b 24 oz a 0.3 a 0.4 a 0.5 a 3.9 ab 6.5 a 1 gal a 0.1 a 0.5 a 0.9 a 5.0 a 6.5 a fludioxonil (NUP09092) 4 oz a 0.1 a 0.3 a 0.5 a 1.0 c 1.9 b fludioxonil (NUP09092) 8 oz a 0 a 0.1 a 0.5 a 0.8 c 1.1 b Thyme oil, 5.6% (Proud 3) 1 gal a 0.4 a 0.4 a 1.0 a 4.9 ab 6.9 a Mandestrobin (S2200) 7.5 oz a 0.1 a 0.1 a 0.4 a 0.6 c 2.4 b Mandestrobin (S2200) 15 oz a 0 a 0 a 0.4 a 0.8 c 1.4 b Benzoviniflupyr + azoxystrobin (Mural) 7 oz a 0.1 a 0.3 a 0.3 a 0.9 c 2.4 b Untreated uninoculated a 0 a 0 a 0.1 a 0.8 c 1.3 b Untreated inoculated a 0.1 a 0.1 a 0.8 a 5.0 a 6.9 a Fenhexamid (Decree) 1.5 lb a 0.1 a 0.1 a 0.5 a 3.5 b 6.6 a a Disease severity was rated on a scale from 0 to 10 (where 0=no symptoms and 10=100% of leaves affected by symptoms). Means within a column with similar letters are not significantly different according to Tukey s HSD (p=0.05)

6 Ornamental Horticulture Program Research Report Form p 5 Researcher: Nora Catlin Date: 11/10/2016 Project Title: Efficacy of Management Tools for Botrytis Protocol #: PRnumbers: 32345, 32346, 32675, 32676, 32679, 32347, Figure 2. Average disease severity rating for lily Dazzle plants treated with various management products and untreated plants. (Disease severity was rated on a scale from 0 to 10 (where 0=no symptoms and 10=100% of leaves affected by symptoms) 8 Lily Disease Ratings /16 7/21 7/27 8/4 8/14 8/20 8/25 9/1 Fluxapryoxad + pyraclostrobin (BAS703 06F) F9110 Bacillus amyloliquifaciens (MBI110) fludioxonil (NUP09092) fludioxonil (NUP09092) Thyme oil, 5.6% (Proud 3) Mandestrobin (S2200) Mandestrobin (S2200) Benzoviniflupyr + azoxystrobin (Mural) Untreated uninoculated Untreated inoculated Fenhexamid (Decree)

7 Ornamental Horticulture Program Research Report Form p 6 Researcher: Nora Catlin Date: 11/10/2016 Project Title: Efficacy of Management Tools for Botrytis Protocol #: PRnumbers: 32345, 32346, 32675, 32676, 32679, 32347, Table 3. Average initial height and width measurements and final height measurements of lily Dazzle plants treated with various management products and untreated control plants. Treatment Rate/ 100 gal Height Width Initial Final Initial Final Fluxapryoxad + pyraclostrobin (BAS703 06F) 8 oz 55.6 a 73.6 a 18.9 a 21.3 a Protein extracted from Lupinus sp., 20% F oz 54.8 a 70.1 ab 18.1 a 21.1 a Bacillus amyloliquifaciens (MBI110) 1 gal 53.5 a 70.5 ab 18.9 a 21.1 a fludioxonil (NUP09092) 4 oz 54.4 a 74.0 a 19.5 a 22.3 a fludioxonil (NUP09092) 8 oz 55.1 a 74.2 a 18.0 a 20.4 a Thyme oil, 5.6% (Proud 3) 1 gal 55.2 a 70.5 ab 18.6 a 20.4 a Mandestrobin (S2200) 7.5 oz 54.1 a 74.3 a 18.0 a 21.1 a Mandestrobin (S2200) 15 oz 56.4 a 75.9 a 18.6 a 22.2 a Benzoviniflupyr + azoxystrobin (Mural) 7 oz 55.4 a 75.3 a 18.8 a 21.9 a Untreated uninoculated 52.4 a 62.1 b 18.3 a 20.1 a Untreated inoculated 57.0 a 76.8 a 19.4 a 21.8 a Fenhexamid (Decree) 1.5 lb 55.1 a 74.2 a 18.8 a 21.0 a Means within a column with similar letters are not significantly different according to Tukey s HSD (p=0.05)

8 Ornamental Horticulture Program Research Report Form p 7 Researcher: Nora Catlin Date: 11/10/2016 Project Title: Efficacy of Management Tools for Botrytis Protocol #: PRnumbers: 32345, 32346, 32675, 32676, 32679, 32347, Table 4. Plant safety evaluation of lily Dazzle plants treated with various management products and untreated control plants. Treatment Rate/ 100 gal 16 July 21 July 27 July Phytotoxicity a 4 Aug 14 Aug 20 Aug Fluxapryoxad + pyraclostrobin (BAS703 8 oz F) Protein extracted from Lupinus sp., 20% F oz Bacillus amyloliquifaciens (MBI110) 1 gal fludioxonil (NUP09092) 4 oz fludioxonil (NUP09092) 8 oz Thyme oil, 5.6% (Proud 3) 1 gal Mandestrobin (S2200) 7.5 oz Mandestrobin (S2200) 15 oz b Benzoviniflupyr + azoxystrobin (Mural) 7 oz b Untreated uninoculated Untreated inoculated Fenhexamid (Decree) 1.5 lb a Individual plants were evaluated and rated for phytotoxicity symptoms using a 0 to 10 scale (0=healthy/no symptom) b Symptoms of bleached, necrotic spots and patches were observed only on flower petals, plants were in full bloom on 13 Aug, 50% out of bloom 19 Aug, and all out of bloom 25 Aug. 25 Aug 1 Sept

9 Ornamental Horticulture Program Research Report Form p 8 Researcher: Nora Catlin Date: 11/10/2016 Project Title: Efficacy of Management Tools for Botrytis Protocol #: PRnumbers: 32345, 32346, 32675, 32676, 32679, 32347, Materials & Methods/Recordkeeping Name(s) of Personnel Conducting Research: Nora Catlin, Kyle Smith Location of Trial (city/state): Riverhead, NY Use Site (greenhouse/shadehouse/field container/etc): field container Crop History Crop Cultivar/Variety: Asiatic lily Dazzle Date of Seeding: n/a Date of Emergence: n/a Date of Transplanting: 17 June Potting Mix: MetroMix 360 Pot size & spacing: 5.5 inch jumbo square Row spacing: n/a Product(s) applied prior to start of experiment: Product Rate Application Type Date of Application Crop Growth Stage Application Volume

10 Ornamental Horticulture Program Research Report Form p 9 Researcher: Nora Catlin Date: 11/10/2016 Project Title: Efficacy of Management Tools for Botrytis Protocol #: PRnumbers: 32345, 32346, 32675, 32676, 32679, 32347, Experiment Information Experimental Design: Number of Reps: CRD 8 single plant replicates Materials & Methods: Lily Dazzle bulbs were planted on 17 June into 5.5 inch pots and kept in greenhouse until after the first treatment. After the first treatment, plants were moved into a hoop house fitted with shade cloth and overhead irrigation in order to provide ideal disease conditions. Containers of symptomatic plants were placed among the plants to serve as inoculum and the untreated uninoculated control plants were set on the opposite side of the house, in an unshaded section a distance away from the inoculum. When necessary, plants were supplemented with hand watering. Treatments were applied every 2 weeks for 7 weeks, starting on 16 July (27 d after planting), with the exception of F9110, Bacillus amyloliquifaciens (MBI110), thyme oil (Proud 3), and fenhexamid (Decree), which were applied weekly. Plants were evaluated for disease symptoms weekly, starting one week after treatment. Where applicable, data were subject to ANOVA and means were separated using Tukey s HSD (p=0.05). Despite these ideal conditions for disease, no to little disease was observed for the first 4 weeks. The protocol had detailed 5 weeks of treatment application, but since few symptoms were occurring the treatments were extended for another 2 weeks. In order to help encourage disease development, the irrigation timing was shifted from a 10 minute morning and 10 minute mid afternoon irrigation, to a 10 minute morning and 10 minute early evening irrigation to provide a longer period of leaf wetness. After this change, disease symptoms developed to a satisfactory level for the last three weeks of the trial. Application Equipment: Treatments were applied using a CO 2 powered sprayer fitted with a TeeJet 8003 nozzle at 30psi. Product(s) applied during experiment (including treatments, fertilizers, etc): Product Rate/ 100 gal Application Type Date of Application Crop Growth Stage Application Volume Fluxapryoxad + pyraclostrobin (BAS703 06F) 8 oz Spray 7/16/15 27 d after planting To drip; 268 ml Protein extracted from Lupinus sp., 20% (F9110) 24 oz Spray 7/16/15 27 d after planting To drip; 244 ml Bacillus amyloliquifaciens (MBI110) 1 gal Spray 7/16/15 27 d after planting To drip; 260 ml fludioxonil (NUP09092) 4 oz Spray 7/16/15 27 d after planting To drip; 262 ml fludioxonil (NUP09092) 8 oz Spray 7/16/15 27 d after planting To drip; 268 ml Thyme oil, 5.6% (Proud 3) 1 gal Spray 7/16/15 27 d after planting To drip; 239 ml Mandestrobin (S2200) 7.5 oz Spray 7/16/15 27 d after planting To drip; 242 ml Mandestrobin (S2200) 15 oz Spray 7/16/15 27 d after planting To drip; 275 ml

11 Ornamental Horticulture Program Research Report Form p 10 Researcher: Nora Catlin Date: 11/10/2016 Project Title: Efficacy of Management Tools for Botrytis Protocol #: PRnumbers: 32345, 32346, 32675, 32676, 32679, 32347, Table, CONTINUED: Product Rate/ 100 gal Application Type Date of Application Crop Growth Stage Application Volume Benzoviniflupyr + azoxystrobin (Mural) 7 oz Spray 7/16/15 27 d after planting To drip; 239 ml Fenhexamid (Decree) 1.5 lb Spray 7/16/15 27 d after planting To drip; 249 ml Protein extracted from Lupinus sp., 20% (F9110) 24 oz Spray 7/21/15 All in bud To drip; 263 ml Bacillus amyloliquifaciens (MBI110) 1 gal Spray 7/21/15 All in bud To drip; 277 ml Thyme oil, 5.6% (Proud 3) 1 gal Spray 7/21/15 All in bud To drip; 268 ml Fenhexamid (Decree) 1.5 lb Spray 7/21/15 All in bud To drip; 268 ml Fluxapryoxad + pyraclostrobin (BAS703 06F) 8 oz Spray 7/28/15 All in bud To drip; 379 ml Protein extracted from Lupinus sp., 20% (F9110) 24 oz Spray 7/28/15 All in bud To drip; 345 ml Bacillus amyloliquifaciens (MBI110) 1 gal Spray 7/28/15 All in bud To drip; 367 ml fludioxonil (NUP09092) 4 oz Spray 7/28/15 All in bud To drip; 379 ml fludioxonil (NUP09092) 8 oz Spray 7/28/15 All in bud To drip; 380 ml Thyme oil, 5.6% (Proud 3) 1 gal Spray 7/28/15 All in bud To drip; 359 ml Mandestrobin (S2200) 7.5 oz Spray 7/28/15 All in bud To drip; 360 ml Mandestrobin (S2200) 15 oz Spray 7/28/15 All in bud To drip; 381 ml Benzoviniflupyr + azoxystrobin (Mural) 7 oz Spray 7/28/15 All in bud To drip; 359 ml Fenhexamid (Decree) 1.5 lb Spray 7/28/15 All in bud To drip; 369 ml Protein extracted from Lupinus sp., 20% (F9110) 24 oz Spray 8/5/15 Bacillus amyloliquifaciens (MBI110) 1 gal Spray 8/5/15 Thyme oil, 5.6% (Proud 3) 1 gal Spray 8/5/15 Fenhexamid (Decree) 1.5 lb Spray 8/5/15 All in bud, <1% in bloom All in bud, <1% in bloom All in bud, <1% in bloom All in bud, <1% in bloom To drip; 356 ml To drip; 380 ml To drip; 356ml To drip; 368 ml Fluxapryoxad + pyraclostrobin (BAS703 06F) 8 oz Spray 8/13/15 Full bloom To drip; 377 ml Protein extracted from Lupinus sp., 20% (F9110) 24 oz Spray 8/13/15 Full bloom To drip; 354 ml Bacillus amyloliquifaciens (MBI110) 1 gal Spray 8/13/15 Full bloom To drip; 379 ml fludioxonil (NUP09092) 4 oz Spray 8/13/15 Full bloom To drip; 375 ml fludioxonil (NUP09092) 8 oz Spray 8/13/15 Full bloom To drip; 375 ml Thyme oil, 5.6% (Proud 3) 1 gal Spray 8/13/15 Full bloom To drip; 356 ml Mandestrobin (S2200) 7.5 oz Spray 8/13/15 Full bloom To drip; 364 ml Mandestrobin (S2200) 15 oz Spray 8/13/15 Full bloom To drip; 377 ml

12 Ornamental Horticulture Program Research Report Form p 11 Researcher: Nora Catlin Date: 11/10/2016 Project Title: Efficacy of Management Tools for Botrytis Protocol #: PRnumbers: 32345, 32346, 32675, 32676, 32679, 32347, Table, CONTINUED: Product Rate/ 100 gal Application Type Date of Application Crop Growth Stage Application Volume Benzoviniflupyr + azoxystrobin (Mural) 7 oz Spray 8/13/15 Full bloom To drip; 357 ml Fenhexamid (Decree) 1.5 lb Spray 8/13/15 Full bloom To drip; 358 ml Protein extracted from Lupinus sp., 20% (F9110) 24 oz Spray 8/19/15 ~50% out of bloom To drip; 356 ml Bacillus amyloliquifaciens (MBI110) 1 gal Spray 8/19/15 ~50% out of bloom To drip; 381 ml Thyme oil, 5.6% (Proud 3) 1 gal Spray 8/19/15 ~50% out of bloom To drip; 360 ml Fenhexamid (Decree) 1.5 lb Spray 8/19/15 ~50% out of bloom To drip; 358 ml Fluxapryoxad + pyraclostrobin (BAS703 06F) 8 oz Spray 8/25/15 All out of bloom To drip; 382 ml Protein extracted from Lupinus sp., 20% (F9110) 24 oz Spray 8/25/15 All out of bloom To drip; 362 ml Bacillus amyloliquifaciens (MBI110) 1 gal Spray 8/25/15 All out of bloom To drip; 381 ml fludioxonil (NUP09092) 4 oz Spray 8/25/15 All out of bloom To drip; 380 ml fludioxonil (NUP09092) 8 oz Spray 8/25/15 All out of bloom To drip; 380 ml Thyme oil, 5.6% (Proud 3) 1 gal Spray 8/25/15 All out of bloom To drip; 362 ml Mandestrobin (S2200) 7.5 oz Spray 8/25/15 All out of bloom To drip; 369 ml Mandestrobin (S2200) 15 oz Spray 8/25/15 All out of bloom To drip; 383 ml Benzoviniflupyr + azoxystrobin (Mural) 7 oz Spray 8/25/15 All out of bloom To drip; 360 ml Fenhexamid (Decree) 1.5 lb Spray 8/25/15 All out of bloom To drip; 366 ml Photos See separate files, listed below: Photo of trial: Trial.JPG Photos of phytotoxicity symptoms observed on flowers, photos taken 14 Aug: On Mural treated plant: Flower Phyto_Mural.JPG, and on S2200 (15 oz) treated plant: Flower Phyto_S oz.jpg Photos of each treatment group at the end of the trial; photos taken 1 Sept: 1 BAS70306.JPG, 2 F9110.JPG, 3 MBI110.JPG, 4 NUP oz.JPG, 5 NUP oz.jpg, 6 Proud 3.JPG 7 S oz.JPG, 8 S oz.jpg, 9 Mural.JPG, 10 Untreated Uninoculated.JPG, 11 Untreatedinoculated.JPG, 12 Decree.JPG

13 Ornamental Horticulture Program Research Report Form p 12 Researcher: Nora Catlin Date: 11/10/2016 Project Title: Efficacy of Management Tools for Botrytis Protocol #: PRnumbers: 32345, 32346, 32675, 32676, 32679, 32347, Data Collected Plants were evaluated and rated for disease severity approximately weekly, starting approximately 1 week after the first treatment. Evaluations were conducted on 16 July, 21 July, 27 July, 4 Aug, 14 Aug, 20 Aug, 25 Aug, and 1 Sept. Disease severity was rated on a scale from 0 to 10 (where 0=no symptoms and 10=100% of leaves affected by symptoms). The numbers of affected leaves were also recorded during evaluations. In addition, any symptoms of phytotoxicity were recorded during the evaluations; symptoms were ranked using a 0 10 scale (0 = no symptoms). Initial plant height and width was recoded approximately 1 week after the first treatment on 20 July, and final plant height was recorded on 8 Sept. Where applicable, data were subject to ANOVA and means were separated using Tukey s HSD (p=0.05). Raw Data See separate Excel file: Efficacy of Mgmt Tools for Botrytis (15 001)

14 Ornamental Horticulture Program Research Report Form p 13 Researcher: Nora Catlin Date: 11/10/2016 Project Title: Efficacy of Management Tools for Botrytis Protocol #: PRnumbers: 32345, 32346, 32675, 32676, 32679, 32347, Environmental conditions during the experiment: From planting until the day after the first treatment, plants were maintained in a greenhouse with an average daily temperature of 77F, an average high of 110F, and an average low of 59F. After the first treatment plants were moved to a shade house, and were irrigated with an overhead sprinkler system for approximately 10 minutes twice per day. From 17 July through 10 Aug plants were irrigated once in the morning and once in the mid afternoon, after 10 Aug plants were irrigated once in the morning and once in the early evening in order to provide conditions more conducive for disease development. When in the container nursery, the average high temperature was 85F and the average low temperature was 67F. There were 5 days with precipitation, totaling 2.12 inches. The following describes the conditions during application of treatments: Application Relative Temperature Date Humidity Conditions 16 July 90F 85% Sunny 21 July 78F 80% Overcast 28 July 78F 80% Sunny 5 Aug 79F 65% Sunny 13 Aug 75F 40% Sunny 19 Aug 83F 70% Partly sunny 25 Aug 81F 60% Sunny

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