TAZO TM Seed Treatment for Turf

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1 TAZO TM Seed Treatment for Turf From: TerraMax, Inc. a better way to grow

2 Looking for Better Root Development Drought Tolerance Yield in mass Top growth Survivability Better Start with TAZO TM

3 What is Azospirillum? A naturally occurring soil bacteria, Azospirillulm produces plant responses that stimulate lateral branching and root development. Azospirillum also fixes atmospheric nitrogen and passes nitrogen directly to the plant. Supplementing fertility.

4 Benefits 1. Improved drought tolerance from larger root mass 2. Improved nutrient uptake from increased root hairs 3. Increased nutrient levels due to fixing of atmospheric N supplements applied N 4. Quicker stand establishment

5 Why Haven t I Heard About Azospirillum? Researchers have known about Azospirillum for several years. However, early products weren t stable enough to provide significant benefits. Google search will find 86,000+ references

6 Why is Stability Important? Bacteria can be sensitive to environmental conditions like heat and moisture. Uninsulated from these elements, their viability may decline before they can impact the crop. Not so with TAZO TM! In A Word Survival!

7 How is TAZO TM Different? TAZO TM uses an advanced stabilization technology that keeps more bacteria viable so consumers receive more benefit. Packaged TAZO TM remains viable for over one year. TAZO TM has been shown to be viable on seed for one year.

8 TAZO TM Shelf Life CFUs/ml (log base 10) TAZO Liquid Shelf Life Temperature in Fahrenheit Start 1 Wk 3 Wks 6 Wks 12 Wks 26 Wks 52 Wks /21/2006 TerraMax copyright

9 Where Does TAZO TM Perform Best? Essentially TAZO is applicable to all areas where grass is grown. Variety testing has been conducted on bluegrass, ryegrass perennial and annual, fescue and bent.

10 How Much TAZO TM? Treating grass seed with TAZO TM : Dry: 1#/cwt TAZO TM Dry 5 # packages Liquid: 8 oz per cwt TAZO TM Liquid 2.5 gallon container TAZO pays in fewer call backs, more satisfied customers and quicker stand establishment

11 Kentucky Blue Grass TAZO TM Vs Control 46 DAP

12 TAZO TM - Dry Bluegrass Root Volume Product TerraMax has developed a seed treatment product that can be used to deliver microorganisms. This is applied as a powder directly on the seed. Description Various concentrations and product formulations were tried in this experiment. Kentucky Bluegrass seed was treated and planted in a soil mixture. The plants were raised under flourescent lights and watered together as needed. Root volumes were measured six weeks after planting Treatment Product: TAZO -Dry Application rate: 1% by weight of seed V olum e in cc Kentucky Bluegrass Root Volume 46 +/ / / / /- 4 Control Mate AZ AVG 2 Mate 2 AZ 22

13 Athletic Field The next slides represent work done by Glen Rehbein Companies at an athletic field installation for a school in Minneapolis. Supervised by Mike Kelly 9/21/2006 TerraMax copyright

14 TAZO TM Treated vs. Non treated Treated Treated Non-treated Treated Non-treated

15 TAZO TM Non Treated vs Treated Non Treated vs Treated

16 TAZO TM Non Treated vs Treated Non Treated vs Treated

17 TAZO TM Non-Treated vs.treated Untreated Bluegrass Ryegrass 14 DAP Treated Bluegrass Ryegrass 14 DAP

18 TAZO TM Non treated vs. Treated Root Volume Untreated Bluegrass Ryegrass 14 DAP Treated Bluegrass Ryegrass 14 DAP

19 University Results - UNL Root mass increase significant Top growth improved Bluegrass is the genus Other sources confirm similar results on other grasses Rate dependent: Increase in rate decrease in root mass 9/21/2006 TerraMax copyright

20 Average Mass (g) with % Variance over control 9/21/2006 TerraMax copyright

21 Benefits.. Increase in root mass Increase in top growth Nitrogen fixation Better stand establishment More plant vigor 9/21/2006 TerraMax copyright

22 Market Opportunity Sod production Overseeding Seed treatment for turf Hydro Seeder Bag seed Bulk seed Range and pasture grasses 9/21/2006 TerraMax copyright

23 TAZO TM Liquid Seed Treatment for Grasses and Turf Contains two strains of Azospirillum Azospirillum lipoferum 10 5 per ml Azospirillum brasilense 10 5 per ml Directions for Use: Use at a rate of 8 ounces per cwt of seed. Spray uniformly on clean seed using a seed treater. Manufactured by: TerraMax, Inc th Street South Cottage Grove, MN CAUTION: KEEP OUT OF REACH OF CHILDREN The purchaser shall be solely responsible for determining the adequacy of this product for their individual and collective needs and application. TerraMax, Inc. makes no express warranties of any kind with respect to this product. Net weight pounds (9.44 kg) 2.5 gallons (9.47 liters) 9/21/2006 TerraMax copyright

24 TAZO TM Dry Seed Treatment for Turf Contains two strains of Azospirillum Directions for Use: Azospirillum lipoferum 10 4 per gram Use at a rate of 1 percent by weight of seed Azospirillum brasilense 10 4 per gram Inert carriers 99.6% by weight Mix well with seed to assure even coverage. Plant as soon as possible after treatment. Manufactured by: TerraMax, Inc th Street South Cottage Grove, MN CAUTION: KEEP OUT OF REACH OF CHILDREN for treating turf. Avoid moisture when mixing. Avoid breathing dust. The purchaser shall be solely responsible for determining the adequacy of this product for their individual and collective needs and application. TerraMax, Inc. makes no express warranties of any kind with respect to this product. Net weight 5 pound (2.27 kg.) 9/21/2006 TerraMax copyright

25 Where Can I Find TAZO TM? Scott Dyer scottdyer@earthlink.net TerraMax Inc th Street South Cottage Grove, MN dakterra2@aol.com

26 TerraMax TAZO TM Greenhouse Root Mass Study Completed by Roch E Gaussoin Phd University of Nebraska Lincoln September 06 9/21/2006 TerraMax copyright

27 Terra Max Greenhouse Study Conducted by UNL Objective To Evaluate relative root growth rate and total root mass of seed treated with Tazo Conclusion: Root mass increase statistically significant Top growth improved Bluegrass in trial Other sources confirm similar results with other grasses 9/21/2006 TerraMax copyright

28 Initial Set Up All 21 Boxes Close Up 9/21/2006 TerraMax copyright

29 Why Cheesecloth? Cheesecloth added on top of the soil to keep moist and prevent drying out. It s s also used to prevent loss of seed during watering. Cloth is removed 1 month after planting. 9/21/2006 TerraMax copyright

30 15 Days after planting 9/21/2006 TerraMax copyright

31 30 Days after planting 9/21/2006 TerraMax copyright

32 Root Tracing Different colors used to indicate different tracing days. This shows how much growth can occur between tracings and which roots are new to each tracing date. The deepest root was marked by the date the root was traced. 9/21/2006 TerraMax copyright

33 Sampling Taking Sample with Cup Cutter Individual Sample 9/21/2006 TerraMax copyright

34 Washing the Sample 9/21/2006 TerraMax copyright

35 Partially Washed off Sample After washing sample with water, some soil still remains. The top growth remains intact and all roots are meshed into each other. The sample is taken to the lab to be further washed. 9/21/2006 TerraMax copyright

36 Finished Cleaned Sample Sample is washed in lab using finer methods and any remaining soil can be picked up and cleaned off. Sample is now ready to be separated. 9/21/2006 TerraMax copyright

37 Separated and Dried Samples Dry 1X Dry 2X Liquid 1X Liquid 2X Spray 1X Spray 2X Control 9/21/2006 TerraMax copyright

38 Average Mass (g) of Dried Samples Roots Rhizomes Top Growth Dry 1X Dry 2X Liquid 1X Liquid 2X Spray 1X Spray 2X Control Rhizome growth was not statistically different at LSD (0.10) 9/21/2006 TerraMax copyright

39 Average Mass per Treatment Average Mass for Dried Samples Mass (g) Treatment Roots Rhizomes Top Growth 9/21/2006 TerraMax copyright

Why Azospirillum? Azospirillum is a naturally occurring soil bacteria that stimulates root development.

Azospirillum Why Azospirillum? Azospirillum is a naturally occurring soil bacteria that stimulates root development. Azospirillum also captures atmospheric nitrogen and converts it to a form of nitrogen