300 series HPLC autosamplers
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1 300 series HPLC autosamplers issue 1
2 Automated HPLC Sampling Manual HPLC sampling can be very difficult and time consuming......hta s range of HPLC autosamplers can perform HPLC sample preparation and injection accurately and efficiently. The autosamplers are innovative, flexible and great value for money. Whatever your HPLC application, the 300 series has the solution for automated injection. The HTA HPLC autosampler range contains autosamplers capable of handling large sample volumes or small sample batches, performing derivatisation, dilution and other liquid handling applications, and carrying out specialised extraction techniques such as MEPS (Micro Extraction by Packed Sorbent) or SPE (Solid Phase Extraction) to suit your analysis needs. About HTA HTA is a major European autosampler and robotics manufacturer based in Brescia, Italy. HTA designs and manufactures scientific instruments and laboratory automation. HTA autosamplers are sold by chromatography companies around the world. LabHut com Autosamplers for: HEADSPACE LIQUID SPME HPLC SPE autosampler vials & accessories The perfect partner for your HPLC Autosampler SERIES THE NEXT GENERATION Enquire for Details 2
3 Contents Page HPLC Sampling Syringe vol. <500µl HT300L Automatic sampler with 110 position sample tray designed to operate with virtually any HPLC system. Performs accurate full or partial loop fill injections. 4 HPLC Sampling for Small Sample Numbers Syringe vol. <500µl HT310L 10 sample version of the HT300L. Ideal for running small sample batches. 5 Multi-Functional HPLC Sampling HT300LPlus Allows virtually any combination of HPLC sample preparation and handling. 6 Automated MEPS & HPLC Sampling MEPS syringe vol. 100µl & 250µl HT300LMEPS Performs automated MEPS (Micro Extraction by Packed Sorbent) in addition to the multiple features of the HT300LPlus HPLC autosampler. 7 Large Volume HPLC Sampling Syringe vol. 1ml & 10ml HT300LV Large volume version of the HT300L, designed for use with syringes of 1ml or 10ml ideal for semi prep HPLC or small volume flash chromatography applications. 8 Very Large Volume HPLC Sampling Syringe vol. 50ml HT300VLV A special very large volume version of the HT300L, for use with samples of 50ml ideal for special applications that require extra large volume injection. 30 position tray. 8 Automated SPE & HPLC Sampling SPE Cartridge vol. 1ml & 3ml HT400E SPE Reliably and accurately automates both SPE (Solid Phase Extraction) and HPLC procedures. 9 Autosampler Control Software HTCOMSoft-L Microsoft Windows based software made for programming and controlling HTA HPLC autosamplers. 10 Specifications Technical details for the HPLC autosamplers. 10 3
4 HT300L Automated HPLC Sampling Works with any HPLC System! The HPLC autosampler HT300L is an automatic sampler designed to operate with virtually any HPLC system. The HT300L can perform full or partial loop injections into the 6-way vertical injection port valve with great precision because the injected quantity is handled by the syringe (and not only by the loop as is the case with pressurebased samplers). The unit uses a standard HPLC syringe and mimics manual injection but with greatly improved RSDs. The communication port of the sampler has a variety of input and output signals to ensure that it can interface with your system. Easy to Use The instrument can be programmed by the integrated keypad, or via a PC using HT-COMSoft-L or DataApex Clarity software. The HT300L HPLC autosampler offers a number of benefits. It is easy to use and yet allows the most sophisticated sampling techniques: Syringe washes with sample and/or solvent Pull up strokes for bubble elimination Controlled filling and injection speeds Sample derivatisation or dilution Process up to 110 samples Priority sample handling Configuration The autosampler s 110-position rotating tray supports vials of 2ml and 2.5ml. 4
5 Key Features Direct syringe injection allows partial or full loop fills Variable fill speed allows for wide range of sample viscosities Optional syringe volumes optimised for specific applications Due to the unit s short flow path, it is able to handle aggressive solvents Low maintenance Biocompatible option available Derivatisation or Dilution The HT300L can perform sample derivatisation or dilution before the sample is injected. The sampler can pick up a reagent from one of the solvent positions, mix it with the sample via a set of mixing strokes then inject the sample into the HPLC system. This can be used to add chromophores or fluorescing agents to make the samples easier to detect. The HT300L has an HPLC valve with variable loop sizes depending on the application. The valve is automatically controlled and powered by the autosampler. HT310L Automated HPLC Sampling for Small Sample Numbers Running Small Sample Batches? Manual injection can be time consuming and very difficult to reproduce accurately. Cost can sometimes prevent people from automating their injection, but the 10 sample HT310L makes it cost effective to automate small sample runs and provides excellent levels of reproducibility while saving valuable time. The HT310L has a fixed Pocket that holds up to 10 sample vials and gives the autosampler a very small footprint. Whatever your HPLC system, the HT310L is compatible and will improve your results. HT310L with 10-position fixed tray 5
6 HT300LPlus Multi-Functional HPLC Sampling When Flexibility Counts Based on the HT300L, the HT300LPlus is an automatic sampler designed to allow virtually any combination of sample preparation and liquid handling that the most demanding HPLC application may require. Using up to 4 reagent vials, the system can move liquid from any vial in any position, to any other vial in any position, with no limit to the number of times, or the number of addition functions. The HT300LPlus can also mix the vial contents, perform dilutions, and even includes a wait function to allow for timed derivatisations or reactions. Once the sample is prepared, it can be injected into any HPLC system using the integral HPLC valve. The autosampler is configured with a 110-position rotating tray for vials of 2 and 2.5ml. You can upgrade your HT300L into a HT300LPlus enquire for details. Key Features In addition to the standard HT300L HPLC autosampler features, the HT300LPlus can: Move liquid from any vial to any other vial Mix vial contents at any time Add wait functions as required to allow for reaction times Inject samples as required into any HPLC system Multiple Functions The HT300LPlus includes all of the functions of the HT300L HPLC autosampler and offers an unprecedented number of benefits. It is easy to use and yet allows the most sophisticated sampling techniques: Syringe washes with sample or with solvent Pull up strokes for bubble elimination Controlled filling and injection speeds Single and multiple sample derivatisation (ph spiking is also supported) Sample dilution Use variable syringe sizes for different applications Dilute samples prior to injection Be comprehensively and easily controlled with QuickPrep software Offline mode for preparative operations without direct injection Samples can be prepared and then injected immediately or prepared as a batch for later analysis Unlimited sample transfer steps Easy to Use The instrument is controlled by user-friendly Quick-Prep software, which allows intuitive drag-and-drop programming. Simply select the function and then select the position where it is to be performed. Complex procedures can be programmed in very little time, making the HT300LPlus ideal for research laboratories and applications where non-routine sample preparations are carried out. There is no practical limit to the number of steps you can program, so even the most complex of operations can be easily controlled. Quick-Prep software for programming the HT300LPlus 6
7 HT300LMEPS Automated MEPS & HPLC Sampling The HT300LMEPS is a proven HPLC autosampler that supports automated MEPS (Micro Extraction by Packed Sorbent) for HPLC in addition to the multiple features of the HT300LPlus HPLC autosampler. What is MEPS? MEPS is a new development in the fields of sample preparation and sample handling. MEPS is the miniaturisation of conventional SPE cartridges from millilitre to microlitre bed volumes. The MEPS approach to sample preparation is suitable for reversed phases, normal phases, mixed mode or ion exchange chemistries. MEPS is available in a variety of common SPE phases. Benefits of MEPS Historically, many sample preparation methods used liquid-liquid extraction (LLE) which required large volumes of sample, solvents and time. The advantages of SPE (Solid Phase Extraction) over LLE are that SPE takes much less time, can be developed into a fully automated technique, requires much less solvent and offers selectivity. MEPS performs the same functions as SPE the removal of interfering matrix components and the selective isolation and concentration of analytes. However, MEPS increases the advantages of conventional SPE in the following ways: MEPS works with much smaller samples than full scale SPE (as small as 10µl) MEPS can be fully automated the sample processing, extraction and injection steps are performed on-line using the same syringe MEPS is applicable for both GC and LC (see our other autosamplers) MEPS significantly reduces the time needed to prepare and inject samples MEPS considerably reduces the volume of solvents needed Syringe and Barrel Insert Needle (BIN) Assembly The MEPS product consists of two parts, the MEPS Syringe and the MEPS Barrel Insert and Needle Assembly (BIN). The BIN contains the SPE phase, and is built into the syringe needle. The BIN is used with a 100µl or 250µl gas tight MEPS syringe that allows fluid handling at normal SPE pressures. The BIN is easily changed when another phase is required or the current BIN is exhausted. MEPS Automation with the HT300LMEPS The HT300LMEPS offers the perfect platform for automated MEPS. The ability to prepare and inject samples on-line using a single device reduces sample processing time and the need for operator intervention. The HT300LMEPS works with all current MEPS syringes. There is no need to modify the syringe mount when using a different sized syringe. 7
8 HT300LV Large Volume HPLC Sampling Using Large Samples of up to 10ml? Ideal for Flash/Semi-Prep or other larger volume applications, the HT300LV is an autosampler designed to operate with virtually any HPLC system. Using a 1ml or 10ml HPLC syringe, the system allows maximum flexibility in handling larger volume samples. The HT300LV comes complete with a 40-position rotating tray for 10ml vials. The unit has solvent and waste nozzles that allow a potentially unlimited supply of solvent to be drawn from large external bottles. 40-position tray HT300VLV Very Large Volume HPLC Sampling Using Very Large Samples of up to 50ml? HTA can custom manufacture autosamplers for special applications. The HT300VLV is specially designed for use with very large syringe volumes for samples of up to 50ml ideal for special applications that require extra large volume injection. Enquire to discuss your custom applications. 30-position tray 8
9 HT400E SPE Automated SPE & HPLC Sampling SPE Preparation and Injection The HT400E can reliably and accurately automate manual SPE (Solid Phase Extraction) procedures and automatically inject samples into an HPLC system. The HT400E can use up to 10 different solvents (4 as standard) to activate cartridges and wash and elute samples in different fractions. It has separate valves for syringe washing and can also add reagents to the eluent before injection. Washing and elution are performed at constant flow (no vacuum or gas pressure). This exceptionally high flow reproducibility and a zero dead volume plunger design ensure the best method for reproducibility and consistency. The HT400E uses standard 1ml or 3ml SPE cartridges and can process up to 20 separate samples sequentially. Each sample can have a multiple step methodology providing the flexibility required for complex processes. Switch between HPLC & SPE Save Money If you save just 1 hour a day in sample preparation then the HT400E will pay for itself in approximately 3 months!* * Based on European average cost per hour of technician. The sample trays easily lift on and off the holder so samples can be conveniently loaded away from the unit. Simply change the sample tray to switch between HPLC and SPE modes. Software Interface Quick-Prep software for the HT400E The HT400E s Quick-Prep software uses an intuitive powerful drag and drop interface to allow the unit to control every individual aspect of the SPE process. The flexibility of Quick-Prep enables the unit to perform a wide variety of applications such as the collection of several fractions of an elution or the ability to re-inject a sample into a different SPE tube if required. 9 HPLC tray SPE tray
10 HT-COMSoft-L Control Software Specifications Stay in Control HT-COMSoft-L is Microsoft Windows based software made for programming and controlling the HTA HPLC autosamplers. The software may be run stand-alone or alongside other Windows software such as HPLC control or data systems. Through HT-COMSoft-L it is possible to: Program the method files Program the sequence files Compile the sample list Print the contents of all files Control the set up Graphically visualise an automatic run Command a single injection HT300L Specifications Any vial may be run using any method, or multiple samples can be programmed using a sequence. Analytical Methods: Up to 10 Sample Volume: Fill Speed: Bubble Elimination: Viscosity Delay: Steps of 0.1µl 1-100µl/sec Up to 15 pull up strokes 0-15 secs Injection Speed: 1-100µl Derivatization Volume: Derivatization Time: Derivatization Function: Needle Wash: Loop Wash: Wash Mode: Linearity: Steps of 0.1µl 0-25 mins Up to 15 repetitions With solvent/sample With solvent/sample Fully programmable Reproducibility: Full loop: 0.3% (typically 0.15%) Partial loop: 0.6% Carry Over: 0.05% Tray Capacity: 110 vials, 2 or 2.5ml (HT310L) 10 vials, 2 or 2.5ml (HT310L) 4 Control Electrical Control: PC Interface (optional), HTCOMSoft, (Also DataApex Clarity) HT-COMSoft-L visualising an automatic run Physical Characteristics Dimensions (W x H x D): 420 x 520 x 400mm Weight: 13.0kg HT-COMSoft-L programming methods 10
11 HT300LPlus Specifications The HT300LPlus can perform all the functions of the HT300L and can additionally be programmed to move any sample from any position for derivatisation or dilution. 6 HT300LMEPS Specifications Same as HT300LPlus except: MEPS Syringe Volume: 100 & 250µl 7 Sample Volume: Air Volume: Aspirating Speed: Needle Washing: Wash Mode: Bubble Elimination: Viscosity Delay: Injection Speed: Waiting Time (before and after inject): Injection Depth: IS Volume: Air Gap Volume: Mode: Tray Capacity: Control Electrical Control: Physical Characteristics Dimensions (W x H x D): Weight: Steps of 0.1µl Steps of 0.1µl 1-100µl/sec Up to 15 strokes Fully programmable Fully programmable Fully programmable µl/sec Fully programmable Variable Steps of 0.1µl Steps of 0.1µl 1 or 2 air gaps 110 vials, 2 or 2.5ml HTCOMSoft 420 x 520 x 400mm 9.0kg HT300LV Specifications Same as HT300L except: Sampling: Sample Volume: 40 vials, 10ml 10ml Other specifications available on request HT400E SPE Specifications HPLC Operation Sample Volume: Syringe Size: Derivatisation Time: Derivatisation Function: Needle Wash: Loop Wash: Tray Capacity: SPE Operation SPE Cartridge Volumes Available: Tray Capacity: 8 Fixed loop, 20µl standard. Partial loop possible 1ml, 2.5ml and 10ml (2.5ml as standard) User defined Choice of reagents With solvent/sample With solvent/sample 110 vials, 2 or 2.5ml 1ml, 3ml 20 samples Tray Temperature Control Option: Peltier system. 10 C below ambient to +40 C Solvent Lines: Up to 10 (4 + wash as standard) SERIES THE NEXT GENERATION Control Electrical Control: Physical Characteristics Dimensions (W x H x D): Weight: Power Supply: RS-232 and TTL 510 x 735 x 400mm 15.0kg 110/220V AC, 50/60Hz Enquire for Details 11
12 Your Local Distributor: SMI-LabHut Ltd SMI-LabHut Ltd The Granary The Steadings Maisemore Gloucestershire GL2 8EY UK Tel: +44 (0) Fax: +44 (0) Website:
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