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2 Sample Prep System J2 Scientific introduces the ultimate flexibility and automation for the sample prep lab. The PrepLinc Platform Automated Sample Preparation System offers the ability to combine common sample prep process inline on one system. With modules available for automating Solid Phase Extraction, Gel Permeation Chromatography Cleanup and Concentration, there are a multitude of configuration possibilities. Combine SPE with Concentration, combine GPC Cleanup with SPE, even concentrate a sample between two processes. The combination of these technologies gives the user flexibility and options to significantly decrease sample handling while increasing data quality and productivity. High powered software utilizes the features of each module to make the PrepLinc a complete sample prep solution, Autosampler - Create unique mat with your choice of tray sizes - Create user-defined trays in the software - Septum piercing is standard SPE - Use up to 15 ml columns; not limited to one manufacturer - Multi-column methods - Positive pressure with programmable flow rates - Forward and reverse flow on any position - Large volume pump option for a variety of water methods 1901 Pennsylvania Drive Suite C (573) (866) AccuVap - Only FULLY automated concentration system - Concentrate samples from SPE & GPC or from vial - Collect multiple fraction in chamber for combined concentration - Quantitate in chamber; transfer to GC vial ready for analysis - Take sample completely to dryness or adjustable endpoint - Chamber options based on final volume GPC Cleanup - Direct Inject; inject entire sample onto column - Use traditional and high pressure columns up to 5500 psi - Integrate with AccuVap or SPE

3 Hardware Configurations SPE Single Column * SPE Multi-Column * GPC Cleanup * Stand Alone Concentration ** GPC Cleanup with Inline SPE Multi-Column* GPC Cleanup with Inline SPE Single Column* * Mode can be combined with optional AccuVap Concentration system ** Requires optional AccuVap FLX System Programming Multi-Module Methods with Linc Editor The Linc Editor makes it possible to linc methods from different modules together to process a sample in one process. The easy drag-and-drop editor is a breeze to use and it really completes the PrepLinc group of products. Autosampler Mat Editor & Capacity GPC with Evaporation 216 Samples GPC Only Mode 84 Samples GPC with SPE Mode Samples SPE Single Column Mode 45 Samples SPE 2 Column Mode 18 Samples SPE 3 Column Mode 9 Samples SPE w/ Evap. Single Column 45 Samples SPE w/ Evaporation 2 Column 18 Samples SPE w/ Evaporation 3 Column 9 Samples The flexible autosampler mat editor and tray editor provide for a variety of sample and collect tray configurations. PrepLinc Sequence Editor Sequences are not limited to one method, one module or one Linc method User can customize sequence as laboratory flow dictates Choose sample and collect tray and location for first sample; sequentially copies for additional samples, but can be edited for unique situations Choose priority samples Re-arrange, add and delete unprocessed samples after run has begun Change method and tray information for unprocessed samples after run has begun To request a quote or locate your nearest dealer, contact Customer Care toll-free in the US at or Pennsylvania Drive Suite C (573) (866)

4 The PrepLinc SPEi from J2 Scientific accentuates our line of full-featured automated sample preparation instruments. The SPEi system uses positive pressure for consistent flow sample to sample and run to run. The intuitive software and range of parameters make method development easy. Converting any manual SPE method to automated takes no time. Use the SPEi as a stand-alone automated SPE system or integrate with other PrepLinc modules, like the AccuVap Evaporation module or GPC Cleanup module for a completely automated sample prep system. Automated Solid Phase Extraction SPE for Environmental, Water, Food, Pharma and Forensics samples Automate a manual SPE method in minutes Adapt to any cartridge style from 1mL to 15mL Standout Features: Use cartridges from 1mL - 15mL plus many specialty and flash chromatography columns Perform multi-column procedures; up to 5 columns inline Flow Inject Module for water/aqueous samples *Coming Soon Concentrate eluate to final volume or between prep processes with our AccuVap Ability to perform forward and reverse elution through cartridges Program up to 12 different solvents in a method Programmable flow rates to 65 mls/min Positive Pressure Elution for Sample and Solvents Nitrogen Drying Closed System with Pressure Monitoring 1901 Pennsylvania Drive Suite C (573) (866) Configuration Options Add up to 5 SPEi column modules - Capacity of 45 samples - Flow from one module to any other module Add column modules as need increases Process with different cartridge sizes on each column module Optional Reverse Elution valve gives increased application flexibility

5 Configuration Options SPE Cartridges The SPEi system will adapt to most manufacturers cartridges. J2 Scientific offers a selection of cartridges, adapters and plungers. PrepLinc Modules Add these modules to your PrepLinc system for increased flexibility and automation. PL GPC Cleanup Module AccuVap Inline or FLX modules - Inline offers GPC + Evap mode - FLX offers GPC + Evap mode and Evap Only mode SPE Method Editor Create simple, single-column or complex, multi-column methods through the same intuitive method editor. The method screen shown at left is an example of typical single column SPE method. There is one condition step, the sample is introduced to the column, a wash step, the sample is dried and then the sample is eluted. This example method is more complicated and shows the real flexibility of the PrepLinc software. Three SPE columns are used for this example. Condition and wash steps are performed to move the sample through all three columns. A reverse elution is performed on one of the columns for the final collection. Both elutions are evaporated using the optional AccuVap and can be collected in separate vials.

6 Adding an AccuVap Concentration Module to the PrepLinc System eliminates the need for a separate bulk evaporation step when combined with processes like SPE or GPC Cleanup. Choose from two models: AccuVap Inline or AccuVap FLX. Both offer concentration of solvent directly from another process (GPC or SPE), but the FLX also allows offline concentration. Unlike other semi-automated evaporation systems, the AccuVap will automatically solvent exchange and quantitatively transfer your sample to a GC vial ready for analysis. Powerful software controls heat and vacuum at every stage of the process to protect analyte recoveries. Programmable heated rinses eliminate carryover. Save time and improve results. Free up lab technicians and decrease sample handling. Adding the AccuVap to your PrepLinc system will really automate your world! Standout Features: Enclosed evaporation chamber Three heating zones with user defined heat & vacuum for each zone Vacuum control from torr Two endpoint settings: dryness or adjustable endpoint (1-5 ml) Manage use of up to three solvents for sample exchange, final solvent and rinsing Add a pre-evaporation spike Add a keeper solution Perform multiple solvent exchanges Heated rinse cycle with choice of solvent ensures no carry-over Transfer concentrated sample into GC vial or other storage vial AccuVap Method Editor The PrepLinc AccuVap Method Editor is full-featured, yet intuitive. Parameters for each stage of the concentration process are clearly separated. Solvents are programmed at software setup for easy recall when programming methods. Heating rates and vacuum settings are adjustable on the fly for quick method development. Knowledgeable applications staff are available to optimize method parameters for matrix, solvent and analytes of interest.

7 AccuVap Options Inline or FLX Model Choose your AccuVap model based on intended uses. Consult the lists below to determine the model best suited for your lab. AccuVap Inline - GPC with Evaporation* - SPE with Evaporation* Optional PL GPC Cleanup Module AccuVap FLX - GPC with Evaporation* - SPE with Evaporation* - Evaporation Only * Requires additional PL options Optional PL SPEi Starter Kit (can add 4 more column modules) Solvent Recovery Module & Chiller Collects and condenses solvent vapor from concentration process for disposal or recycling SRM Condenser Module Condenses solvent vapor and drains to vessel for disposal or recycling. Collects up to 95% of solvent vapors Immersion Chiller Chiller options for SRM module. 140 W at -30 o C Evaporation Chamber Options Choose an evaporation chamber based on the desired final volume. AV081 - Standard For final volumes from 1 ml to 10 ml AV082-5mL Tip For final volumes from 0.5 ml to 5 ml AV ml Tip For final volumes from 0.5 ml to 5 ml For small volume and single addition concentrations To request a quote or locate your nearest dealer, contact Customer Care toll-free in the US at or Pennsylvania Drive Suite C (573) (866)

8 The GPC Cleanup Module for PrepLinc performs cleanup of a wide range of sample matrices including foods, tissues, grains, plants and environmental samples such as soil, sludge, and hazardous waste for EPA (SW-846 Method 3640A), CLP, FDA, USDA and USDI analysis. Using our experience with GPC Cleanup, J2 Scientific has perfected it on this new platform. All the unique features of previous models are combined with highpowered software and the ability for further automation by combining GPC with other prep processes on the PrepLinc. The PrepLinc GPC Cleanup Module provides another level of functionality to the PrepLinc system. The ability to combine GPC Cleanup with Automated Concentration and/or SPE on the PrepLinc system, completes the sample prep package. Standout Features: Direct Inject - inject the entire sample onto the column Septum piercing standard for sample & collect vials Probe tracking & user-defined rinses 2- or 5-column selector valve UPS Power Watchdog Sample Dilution Multiple Injections Specialized calibration & reporting software features for GPC Create methods directly from the column calibration file Use with low-pressure glass or high pressure columns Integrate with PrepLinc SPE and AccuVap modules GPC Method Editor Use the defined method templates for standard GPC Cleanup and Column calibration or create a specialized method with multiple collect or dump fractions using the simple method builder. Programmable dilution prior to injection Different flow rate during equilibration User defined rinses Collect fraction flow directed to SPE column* Collect fration flow directed to AccuVap* Program multiple collect fractions; direct each to vial, SPE or AccuVap individually* *requires optional modules

9 Configuration Options Autosampler Options Choose the autosampler size based on desired batch capacity and level of integration with other PrepLinc modules. Choose Model 3300 autosampler for capacities up to 72 samples per batch and ingetration with up to two SPEi column modules. Choose Model AS4 autosampler for capacities up to 200 and full SPEi integration. with Model 3300 with Model AS4 PrepLinc Module Options The following PrepLinc modules can be added for integration with the GPC Cleanup module. PrepLinc SPEi Starter Kit - Add two column modules with M3300 autosampler - Add five column modules with AS4 autosampler AccuVap Inline or FLX modules - Inline offers GPC + Evap mode - FLX offers GPC + Evap mode and Evap Only mode GPC Cleanup Reports The PrepLinc software makes keeping track of your column calibration and sample data easy. Create Method and Configuration reports to report sytsem setup. Create Sequence reports with time/ date stamps. Create Calibration and Comparison reports in compliance with Method 3640a and CLP guidlines. Quickly determine if a column calibration has met pass/fail requirements and print a report to prove it! To request a quote or locate your nearest dealer, contact Customer Care toll-free in the US at or Pennsylvania Drive Suite C (573) (866)

10 The AccuVap EVS Evaporation System is a fully automated way to concentrate offline samples ranging in volume from a few to hundreds of milliliters of solvent. The AccuVap EVS can replace more manual evaporation methods like kudera-danish, rotary evaporation and other semi-automated techniques.. The AccuVap EVS performs solvent exchange and quantitative transfer. A new way to concentrate! The AccuVap EVS combines gentle heat and vacuum to concentrate large volumes of solvent. Precise control devices monitor solvent volume, temperature and vacuum to ensure controlled evaporation. Concentrate samples after PFE extraction. Full automation for concentrating overnight. Concentrate large volumes after liquid-liquid extraction. Fully automates most other evaporation techniques. Standout Features: Enclosed evaporation chamber Three heating zones with user defined heat & vacuum for each zone Vacuum control from torr Two endpoint settings: dryness or adjustable endpoint (1-5 ml) Manage use of up to three solvents for sample exchange, final solvent and rinsing Add a pre-evaporation spike Add a keeper solution Perform multiple solvent exchanges Heated rinse cycle with choice of solvent ensures no carry-over Transfer concentrated sample into GC vial or other storage vial AccuVap EVS Method Editor The PrepLinc AccuVap EVS Method Editor is full-featured, yet intuitive. Parameters for each stage of the concentration process are clearly separated. Solvents are programmed at software setup for easy recall when programming methods. Heating rates and vacuum settings are adjustable on the fly for quick method development. Knowledgeable applications staff are available to optimize method parameters for matrix, solvent and analytes of interest.

11 AccuVap EVS Options Autosampler Options Choose the autosampler model based on capacity need. The Model 3300 autosampler offers 72. The Model AS4 offers 216. with Model 3300 with Model AS4 Solvent Recovery Module & Chiller Collects and condenses solvent vapor from concentration process for disposal or recycling SRM Condenser Module Condenses solvent vapor and drains to vessel for disposal or recycling. Collects up to 95% of solvent vapors Immersion Chiller Chiller option for SRM module. 140 W at -30oC Evaporation Chamber Options Choose an evaporation chamber based on the desired final volume. AV081 - Standard For final volumes from 1 ml to 10 ml AV082-5mL Tip For final volumes from 0.5 ml to 5 ml AV ml Tip For final volumes from 0.5 ml to 5 ml For small volume and single addition concentrations To request a quote or locate your nearest dealer, contact Customer Care toll-free in the US at or Pennsylvania Drive Suite C (573) (866)

12 1901 Pennsylvania Drive Suite C (573) (866) Dual-Loop Manual GPC Cleanup System The PrepLinc GPC Jr Dual Loop Manual Gel Permeation Chromatography Cleanup System meets the needs of labs with few GPC Cleanup samples to process. Perfect for the cleanup of a wide range of sample matrices including foods, tissues, grains, plants and environmental samples such as soil, sludge, and hazardous waste for EPA (SW-846 Method 3640A), CLP, FDA, USDA and USDI analysis. This manual version of our popular PrepLinc GPC shares many of the features of its big brother! Jr boasts a unique dual-loop design that allows continuous processing without an interruption between samples like most manually loaded systems. By sounding an audible alert, PrepLinc GPC Jr lets the user know when the next sample can be loaded, without interrupting the current sample that is processing. The PrepLinc GPC Jr is controlled via intuitive software. An integrated PC is included with the system, giving all the benefits of Windows-based software without adding a PC to your bench top. Easy-to-navigate screens, make programming method data simple. Methods are saved for repeated runs. The solvent pump is controlled for flow rate and automatic shutdown at the end of the sample run. The PrepLinc GPC Jr Dual-Loop GPC Cleanup System is compatible with all standard GPC Cleanup columns, including the traditional low-pressure glass columns and high-pressure stainless steel columns. The Jr version can also be easily upgraded to the standard highcapacity, fully automated PrepLinc GPC to grow with your laboratory. For a personalized quotation call GPC. Dual-Loop Injection Module Injection Volume: 2.5 ml or 5 ml Standard (Custom Loops Available) Injection Types: Loop Over-Fill or Partial Loop Fill Solvent Compatibility: All organic solvents Control Internal Windows PC with PrepLinc GPC Jr Software Define method parameters and pump flow rate, view & annotate chromatograms, save reports (includes monitor, keyboard and mouse) Solvent Pump Integrated High-Pressure Solvent Pump psig Flow Rate: ml/min Pulse-dampened Detector* Fixed wavelength detector available with a 254nm or 280nm lamp. Save money by using a detector you already own. Call to verify compatibility. Columns* Traditional Glass Column Express Performance Column Envirosep-ABC Column (by Phenomenex) Other low- and high- pressure columns (pressure not to exceed 5000 psi) Dimensions Injection module (shown) 22 H x 13 W x 14 D (incl. column bracket) Hub Control Module (not shown) 13 H x 5 W x 10 D Power Requirements VAC 50/60Hz, 2 AMP *Optional

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