Micropropagation Technology Park. Sanjay Saxena

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1 Micropropagation Technology Park Sanjay Saxena

2 Micropropagation Technology Park at Gurgaon, Haryana

3 MTP MTP is one of the largest tissue culture production units in north India with an annual production capacity of 1.5 million plants The facility is ISO 9001 certified and DBT, Govt. of India accredited reflecting the quality control and assurance of the lab Micropropagation protocols have been developed for over 90 species including, forestry species, fruit crops, cash crops, medicinal and aromatic plants, and ornamentals Over 19 million plants have so far been dispatched

4 MTP Large-scale multiplication of superior clones of various species using tissue culture Mass propagation of species that are difficult to regenerate by conventional methods Clonal fidelity of tissue cultured plants is ensured through periodic testing by molecular markers TC plants are further fortified with suitable mycorrhiza for better establishment in the field and improved growth

5 Media lab

6 Autoclave room

7 Autoclaving

8 Inoculation Room

9 Growth Room

10 Greenhouse

11 Greenhouse

12 Polyhouse: Low-cost hardening solution

13 Shadehouse

14 Plants growing in the open nursery

15 Forestry species Anogeissus latifolia Anogeissus pendula Bambusa bambos Bambusa balcoa Bambusa nutans Dendrocalamus asper Dendrocalamus strictus Eucalyptus camaldulensis Eucalyptus citriodora Eucalyptus tereticornis Leucaena hybrids Paulownia fortunei Populus deltoides Vegetable Crops Asparagus Ginger Leek Potato Fruit Species Medicinal & Aromatic Plants Apple (Rootstock) Aloe Vera Banana Asparagus racemosus Citrus (Rootstock) Bacopa monnieri Strawberry Centella asiatica Pineapple Chlorophytum borivilianum Crataeva nurvala Cash Crops Geranium Hops Patchouli Sugarcane Rauwolfia serpentina Turmeric Stevia Black pepper Swertia chirata Large cardamom Vanilla Ornamentals (foliage and flowering) Aglaonema Ficus Hibiscus Alpinia Miscanthus Pinguicula Calathea Nandiana Plumbago Dieffenbachia Philodendron Rose Dracaena Syngonium Thalictrum Drosera Yucca Verbascum

16 Major clients DOMSETIC MARKET Forest & Horticulture departments R&D projects supported by DBT, Govt. of India Agro-based companies Private entrepreneurs Nurseries/Individual farmers/growers INTERNATIONAL MARKET Countries where plants are exported : The Netherlands, Germany, South Africa & Australia

17 Fruit crops

18

19

20 Strawberry

21 Strawberry cultivation near Shillong

22 Pineapple in Assam

23 Citrus

24

25 Apple (rootstock) Shoot multiplication Rooting

26 Shoot multiplication (Scion wood) Scarlet Gala Vance Delocious

27 Cash crops

28 Potato

29 Potato minitubers

30 Potato cultivation at Supi

31 Farmers meet in Uttarakhand

32 Sugarcane

33 Ginger

34 Turmeric

35 Black pepper

36 Large cardamom

37

38 Asparagus

39 Medicinal plants

40 Swertia chirata (Chirayta) Endangered, annual herb found in temperate climate at an altitude of 1,200-1,500 m Prized as a tonic; Used in chronic fevers, anemia, bronchial asthma, liver disorders, anti-malarial, anti- helminthic & other diseases

41 a b c d Swertia chirata

42 Rauwolfia serpentina Picrorhiza

43 Valeriana Chlorophytum

44 Stevia rebaudiana Multiplication Rooting

45 Bacopa monnieri (Brahmi) Shoot multiplication Rooting

46 Forest species

47 Bambusa balcooa

48 Dendrocalamus asper Shoot multiplication Rooting

49 Foliage

50 Representative ISSR fingerprint profiles of in vitro propagated plantlets of S. chirayita

51 Services offered by MTP Supply of tissue cultured plants of various species in the domestic and the international markets Contractual research/production for : - Development of micropropagation protocols for new crops - Refining of existing protocols for making them suitable for appropriate business needs - Mass production and supply of plants on an exclusive basis Technology transfer

52 Role of government NO reinventing of wheel NO ornamentals and species like banana, etc Focus on : - Certified seed program (potato, ginger, turmeric, etc) - Grafted crops (apple, citrus, etc) - Ecologically important crops - Endangered/threatened crops - Introduction and multiplication of exotic germplasm of high potential

53 Role of government Multiplication of parental lines for hybrid seed production Early commercialization of newly bred lines Multiplication of transgenics Supply of virus-indexed mother cultures of superior individuals Transfer of proven technologies to the industry/ entrepreneurs Setting-up of incubation centres

54 Role of government Impart training for large-scale production of plants by tissue culture Demonstration trials for testing technology and creating awareness Subsidy Setting standards for TC companies

55 Other aspects Selection Chemoprofiling Virus indexing Clonal fidelity Mycorrhiza

56

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