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1 S m a r t P r e p O i l & F u e l E x t ra c t o r S y s t e m s Solid phase extraction (SPE) of transformer oils, biofuels, edible, and essential oils can offer challenges of working with difficult matrices, multiple methods, and hazardous solvents. The SmartPrep Oil & Fuel Extractor systems are complete with racks, sample tubes, and accessories packaged to automate the typical manual SPE oil and fuel procedures, removing the challenges of working with oils and fuels, while providing a consistent and simple approach to running multiple methods on the same platform. Order all the parts and accessories needed at one time Reduced learning curve for rapid implementation SmartPrep Extractor and in accordance with your developed methods or regulatory methods (i.e., EPA, FDA, USDA, AOAC and ISO). Monitor according to ASTM and IEC methods: Furan levels for maintenance and safety PCB levels to meet regulatory requirements for safety Perform analysis to meet formulation and quality tests: FAME and MAGs for biofuel quality requirements Oligosaccharides quantity for formulation research Regulate levels of contamination and determine quality: Label conformity ensures best product and quality Pesticides and PAHs are tested for trace levels
2 The Importance of Sample Preparation: integral part of final analysis quality and reliability in final data solid phase extraction (SPE) is commonly used for sample preparation due to its ability to use selective chemistry simplifying sample preparation is the first step to improving accuracy and reproducibility Typical Oil & Fuel Sample Preparation Process & Highlighted Areas for Horizon Technology SmartPrep Extractor Automation and XcelVap Evaporation for Drying Accuracy of SPE volume delivery over a standard curve range of atrazine (10 ng/l 500 ng/l) in water was evaluated. Atrazine, while banned in some countries, is a commonly used herbicide for the control of weeds in corn crops used to produce biofuels and edible oils. The % RSD for seven samples was <5%. P a ge 2
3 Diluted Transformer Oil Sample & Final SPE Fraction Polyaromatic Hydrocarbons (PAH) in Olive Oil Supelco SupelMIP SPE PAH Method 1. Condition with 1 ml cyclohexane 2. Load diluted oil sample 3. Wash with 1 ml cyclohexane 4. Elute with 3 x 1 ml ethyl acetate 5. Evaporate SPE eluate under nitrogen at 40 C and reconstitute in 0.2 ml ethyl acetate for analysis Automated with the SmartPrep Oil & Fuel Extractor Information from Supelco Analytical Application Note 192 Automated SPE - Furans in Transformer Oil 1. Condition cartridge using 5-mL of Hexane at 10-mL/min. Soak 5 sec Sample Dilution SPE Procedure Post SPE Cleanup 2. Clean Sample line using 5-mL of Hexane at 10-mL/min Dry 30 sec 3. Clean sample line using 5-mL of Hexane at 10-mL/min Dry 30 sec 4. Load 20-mL of sample at 3-mL/min. Deliver at 3-mL/min. 5. Wash cartridge using 20-mL of Hexane at 5-mL/min. Soak 10 sec, Dry 300 sec 6. Clean plunger using 6-mL of IPA at 10-mL/min 7. Clean plunger using 1-mL of VENT at 10-mL/min 8. Clean plunger using 6-mL of 20/80/ACN/H2O at 10-mL/min 9. Elute cartridge using 2.5-mL of 20/80 ACN/Water at 5-mL/min into 1st Collection Tube 10. Elute cartridge using 1-mL of VENT at 5-mL/min into 1st Collection Tube Soak 5 sec 11. Clean plunger using 6-mL of IPA of 10-mL/min. Soak 5 sec 12. Clean plunger using 6-mL of IPA at 10-mL/min. Soak 5 sec 13. Clean plunger using 1-mL of VENT at 5-mL/min P a ge 3
4 Common SPE Cartridges Used for Oil & Fuel Applications Sample Matrix Compounds Typical SPE Cartridge Transformer Oils Biofuels Furans per ASTM-D5837 or IEC 61198: 5H2F 2FOL 2FAL 2ACF 5M2F Polychlorinated biphenyls (PCBs) per ASTM-D4059 or EN Fatty Acid Methyl Esters (FAME) per modified ASTM D6584 Mono Acetyl Glycerides (MAGs) per modified ASTM D6584 Oligosaccharides Florisil /SCX dual layer Porous Graphitized Carbon (PGC) Tocopherols Polycyclic Aromatic Hydrocarbons (PAH) Organophosphorous pesticides Organochlorine pesticides MIPs Envi-carb, Diol PSA/C18 dual layer Tocopherols Tocotrienols Sterols C18 Edible Oils Beta Sitosterol Cation Exchange Phenolics C18, NH2 Phosopholipids, C18, Amino-propyl Chlorophyll pigments C18 Flavones Diol Lignans Diol Aflatoxin B1, B2, G1, G2 IAC,, MIPs Zearaleonone IAC, MIPs Essential Oils Alcohols and derivatives Hydrocarbons Carbonyl compounds Ethers Esters Tertiary alcohols Primary alcohols Acids Diols /Amino P a ge 4
5 The SmartPrep Oil & Fuel Extractor Systems are already configured to include the necessary components needed to order a complete system for automated SPE using either a 6-mL cartridge or a 3-mL cartridge. Order the system that fits the ASTM, EU, or SPE method(s) that will be automated. If both 6-mL and 3-mL cartridges will be used, just ask about the additional accessories to include. Quantity Part Number Description 6 ml System 1 SPE-2600 Kit, SmartPrep w/ 6 ml plunger Kit, SPE Start w/o controller Kit (12), Sip Tube, 5, Kynar Kit (12), Sip Tube, 10, Kynar Clear glass bottles w/ caps (500 ml case of 12) Kit, 3.1-micron inline filter mL cleaning cartridges Kit, 2x6 20-mL VOA collect rack Collection vials, 20-mL VOA vials (case of 72) Training at customer s facility mL conical bottom, graduated tube, case (12) mL conical rack liter plastic carboy for waste collection Containment Tray 1 Supplied via ASTM and EU Furan Transformer Oil Software Methods 1 p/n Power cord appropriate for country Upgrade, Oil Waste Lines (FEP) Upgrade, Sample Valve Lines (FEP) Quantity Part Number Description 3 ml System 1 SPE-2300 Kit, SmartPrep w/ 3 ml plunger Kit, SPE Start w/o controller Kit (12), Sip Tube, 5, Kynar Kit (12), Sip Tube, 10, Kynar Clear glass bottles w/ caps (500 ml case of 12) Kit, 3.1-micron inline filter mL cleaning cartridges mL cartridge carousel insert adapters (15 Pk.) Kit, 2x6 20-mL VOA collect rack Collection vials, 20-mL VOA vials (case of 72) Training at customer s facility mL conical bottom, graduated tube, case (12) mL conical rack liter plastic carboy for waste collection Containment Tray 1 Supplied via ASTM and EU Furan Transformer Oil Software Methods 1 p/n Power cord appropriate for country Upgrade, Oil Waste Lines (FEP) Upgrade, Sample Valve Lines (FEP) PN _02 16 Northwestern Drive, Salem, NH USA Tel: (603) Support-Service@horizontechinc.com
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