SPE SPE. Solid Phase Extraction 3. Strong bases strata-x-cw, p.12 Acids strata-x-aw, p.13 On-line extraction cartridge Strata C18 20 x 2.0 mm, p.

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1 SPE SPE Solid Phase Extraction 3 Essential for mproved Sample Preparation: Strong bases strata-x-cw, p.12 Acids strata-x-aw, p.13 On-line extraction cartridge Strata C18 20 x 2.0 mm, p.17 Strata SPE Formats... 4 Material Characteristics... 5 Phase Selection by Manufacturer... 5 Essential for SPE Method Development Selecting the Proper Phase... 6 Selecting the Proper Tube Dimensions... 7 Method Development Software... 8 strata-x Polymeric Sorbents... 9 strata-x strata-x-c strata-x-cw strata-x-aw Strata Ordering nformation Tubes Strata Giga Tubes Strata High-Throughput 96-Well Plates Protein Precipitation Plates Accessories On-line Format Applications Sepra Bulk Sorbents Other SPE Products 3M Empore Extraction Disks and Disk Cartridges Extraction Plates Protein Precipitation Plates SPE Vacuum Manifolds... 22

2 SOLD PHASE EXTRACTON Strata Solid Phase Extraction Strata SPE (Solid phase extraction) Strata Sample Preparation Products and Sepra Bulk Sorbents Ultra-pure sorbents manufactured by Consistently high recoveries for accurate quantitation of analytes Lot-to-lot reproducibility Certificate of analysis supplied with each product Very tight particle distribution yields optimized flow rates Outstanding application support f Strata SPE or Sepra bulk products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE product for FREE! Formats For Traditional Sample Preparation For High-Throughput Screening For Protein Precipitation For Flash and Large Scale-Up For Flash Analysis For On-line Screening strata-x now available in on-line format See p. 17 for ordering information Strata is a registered trademark of, nc. strata-x is a trademark of, nc.

3 Strata SPE (Solid phase extraction) Material Characteristics Particle Size Pore Size Surface Area Carbon Load Bonding End Capping onic Capacity Phase (µm) (Å) (m 2 /g) (%) (meq/g) C18-E trifunctional Yes C18-U trifunctional No C18-T trifunctional Yes C trifunctional Yes Phenyl trifunctional Yes CN trifunctional No NH trifunctional No 1.3 SCX trifunctional No 0.9 WCX trifunctional No 0.8 SAX trifunctional No 0.9 Si-1 (Silica) FL-PR (Florisil ) Polymeric SDB-L strata-x strata-x-c strata-x-cw strata-x-aw SOLD PHASE EXTRACTON Strata Solid Phase Extraction Mixed Mode Screen-C GF proprietary trifunctional Screen-C proprietary trifunctional Screen-A proprietary trifunctional ABW Phase Selection by Manufacturer Waters Varian Supelco JT BAKER ST Macherey-Nagel STRATA ** Sep-Pak Bond Elut Discovery UCT Bakerbond SOLUTE Chromabond Reversed Phase C18-E tc18 C18 DSC-18 C18 Octadecyl C18 (EC) C18ec C18-U PolarPlus C18 C18 Octadecyl Lightload C18 PAH C18-T C18 DSC-18Lt C8 C8 C8 DSC-8 C8 Octyl C8 (EC) C8 Phenyl (PH) PH DSC-PH Phenyl Phenyl PH (EC) SDB-L ENV DSC-PS/DVB H 2O-phobic DVB 101 HR-P LMS PS-RP strata-x Oasis HLB NEXUS StyreScreen DVB H 2O-philic DVB ENV+ Easy Porapak RDX PPL FOCUS Normal Phase Si-1 (Silica) Silica DSC-Silica Silica Silica Gel Silica SiOH FL-PR (Florisil ) Florisil Florisil Florisil Florisil FL Florisil NH 2 NH 2 NH 2 DSC-NH 2 Amino Propyl Amino NH 2 NH 2 CN CN CN-U DSC-CN CN Cyano CN CN on Exchange ABW ENVOLUTE ABN SAX SAX DSC-SAX Quarternary Amine Quarternary Amine SAX SB SCX SCX DSC-SCX Benzene Sulfonic Acid Aromatic Sulfonic Acid SCX SA strata-x-c Oasis MCX StyreScreen DBX strata-x-cw Oasis WCX strata-x-aw Oasis WAX WCX CBA DSC-WCX Carboxylic Acid Carboxylic Acid CBA PCA Screen-C Certify Clean Screen DAU Narc-2 HCX Drug Screen-C GF Certify HF Xtract DAU Screen-A Certify Clean Screen THC Narc-1 HAX **Recommended Alternative - This category indicates an alternative phase which may yield somewhat different selectivity but may also lead to improved resolution.

4 Strata SPE method development SOLD PHASE EXTRACTON Strata Method Development Selecting The Proper Phase SPE sorbents are most commonly categorized by the nature of their primary interaction or retention mechanism with the analyte of interest. The three most common extraction mechanisms used in SPE are reversed phase, normal phase and ion exchange. This concise diagram is designed to assist you in selecting the proper sorbent for your application. For more detailed information, reference books are available (page 372). Mechanism Reversed Phase Normal Phase on Exchange Typical Application Extraction of hydrophobic or polar organic analytes from aqueous matrix Extraction of polar analytes from nonpolar organic solvents Extraction of charged analytes from aqueous or non-polar organic samples aqueous organic water organic organic ionic neutral neutral neutral ionic (+) basic (-) acidic polar** moderately** polar non** polar non polar moderately polar polar (+) basic (-) acidic cation exchange anion exchange reversed phase* reversed phase reversed phase reversed phase normal phase normal phase cation exchange anion exchange strata-x-c strata-x-aw strata-x strata-x strata-x strata-x Si-1 CN strata-x-c strata-x-aw strata-x-cw SAX CN C8 SDB-L SDB-L NH 2 Si-1 strata-x-cw SAX SCX NH 2 CN C18 C18 FL-PR SCX NH 2 WCX Screen-A C8 C8 NH 2 WCX Screen-A Screen-C PH PH Screen-C Screen-C GF Screen-C GF *NOTE: These can be difficult to extract. Consider non-retentive reversed-phase to remove interference. ** Polarity Log P Polar log P<1 Mid-Polar (moderately) log P=1-3 Non-Polar log P>3 log P is the partition coefficient that can be used to determine the polarity of a compound For the latest updates in SPE method development, technical support, and troubleshooting, visit

5 Strata SPE method development Selecting The Proper Tube Dimension Reservoir = Sample Capacity (i.e. 1 ml, 3 ml, 6 ml, 12 ml, 20 ml, 60 ml, 150 ml) The column volume refers to the dimensions of the SPE tube. t determines the largest volume solvent or sample that can be delivered per aliquot. Sorbent Bed Mass = Analyte Capacity (i.e. 30 mg, 50 mg, 60 mg, mg, 150 mg, 200 mg, 500 mg, 1 g, 2 g, 5 g, 10 g, 20 g, 50 g, 70 g) SOLD PHASE EXTRACTON Strata Method Development The sorbent bed mass determines the maximum amount of analyte that can be retained by the sorbent. Reduce breakthrough by selecting the proper bed mass! General Rule to Determine Proper Bed Mass Silica-based sorbents Non-ionic sorbents retain a mass of solute (analyte plus retained contaminants) that is equivalent to approximately 5 % of the sorbent mass. Therefore, a mg cartridge can retain approximately 5 mg of total solute mass. Polymeric sorbents The large surface area of polymers give a higher capacity that is approximately % of the sorbent mass. on exchange sorbents Capacity is determined by the number of charged sites on the sorbent available to interact with the analyte. The mean capacity ranges from meq/ g as shown on page 5. Since the majority of SPE is performed on relatively dirty samples that contain many potential contaminants, it is common practice to be conservative and choose a slightly larger sorbent mass. Bed Volume: the sum of the interstitial volume plus the pore volume of the sorbent. The bed volume of the sorbent determines the solvent volumes required in a SPE method. n general, solvent volumes between 4 to 8 times the bed volume are typically necessary to ensure proper conditioning, washing and elution. Conditioning with less than 4 to 8 bed volumes increases the risk of incomplete solvation of the bed and low or irreproducible recoveries, while more than 4 bed volumes is typically unnecessary. Pore Size: the average diameter of the porous opening on the surface of a solvent particle. For a compound to be extracted in a SPE method, the molecule must not be too large to fit into the pores. For large molecules, sorbents with extra large pores must be used. The average pore size for the Strata sorbents is given on page 5. Conventional packed bed, silica-based SPE products typically have a bed volume of approximately 150 µl per mg of sorbent. Polymeric sorbents require larger solvent volumes for proper conditioning, washing and eluting. t is recommended to use µl per mg of sorbent. For the latest updates in SPE method development, technical support, and troubleshooting, visit

6 Strata SPE method development SOLD PHASE EXTRACTON Strata Method Development Method Development Software nstantly develops analyte specific SPE methods on strata-x sorbents Generates methods for traditional and high-throughput screening Provides fast, accurate, and simple SPE method development Suggested Solid Phase Extraction Method Download trial version at ORDERNG NFORMATON strata SPE Method Development Software Part No. Description Unit Price AA strata SPE Method Development ea Software Program on CD-ROM Method Development Kits For the latest updates in SPE method development, technical support, and troubleshooting, visit Three techniques to choose from... Sample cleanup and concentration High-throughput screening Protein precipitation ORDERNG NFORMATON Sample Preparation Method Development Kits Part No. Description Unit Price strata-x Method Development Kit for Sample Cleanup and Concentration KS strata-x 200 mg/3 ml tubes (10 ea) ea strata-x-c 200 mg/3 ml tubes (10 ea) strata-x-cw 200 mg/3 ml tubes (10 ea) strata-x-aw 200 mg/3 ml tubes (10 ea) Strata Method Development Demo Software strata-x Method Development 96-Well Plate Kit for High-Throughput Screening KS strata-x 10 mg/well (3 rows) ea strata-x-c 10 mg/well (3 rows) strata-x-cw 10 mg/well (3 rows) strata-x-aw 10 mg/well (3 rows) KS strata-x 30 mg/well (3 rows) ea strata-x-c 30 mg/well (3 rows) strata-x-cw 30 mg/well (3 rows) strata-x-aw 30 mg/well (3 rows) Strata Starter Kit for Protein Precipitation CE Strata mpact Protein Precipitation Plate (2 ea) ea Collection Plate 2 ml (2 ea) Sealing Mat (2 ea)

7 ) ) ) ) Strata -X POLYMERC SORBENTS strata-x Polymer Surface Modified StyreneDivinylbenzene SOLD PHASE EXTRACTON Polymeric Surface Modification Better Selectivity for Polar and Non-Polar Compounds TM High Analyte Capacity Better Retention for Target Analytes Deconditioning Resistant Better Reproducibility from Extraction to Extraction Material Characteristics Particle Size Pore Size Surface Area onic Capacity Phase (µm) (Å) (m2 /g) (meq/g) Polymeric strata-x strata-x-c strata-x-cw strata-x-aw Strata-X Polymeric Sorbent strata X Solid Phase Extraction (SPE) Methods strata-x strata-x-c strata-x-cw strata-x-aw Reversed Phase Strong Cation Exchange & Reversed Phase Weak Cation Exchange & Reversed Phase Weak Anion Exchange & Reversed Phase )n Ṅ. )On O S O O - )n O OH )n NH NH 2 Polar and Non-Polar Condition: With methanol Equilibrate: With water Load Sample: Phosphoric Acid (2 % of total volume) can be used to disrupt drug and protein interaction Wash: With 5-60 % methanol in water (organic concentration used in wash can vary based on nature of analyte) Dry for 1 minute Elute Analyte: With methanol/acetonitrile (50:50) Bases Condition: Equilibrate: Load Sample: Wash 1: Wash 2: Elute Analyte: With methanol With water Acidified with 2 % Phosphoric Acid With 0.1 N HC With methanol (elutes acidic and neutral analytes) This helps in removing endogenous contaminants from biological sample matrices, and eliminates ion suppression in LC/MS under ES mode. With 5 % ammonium hydroxide/methanol* or acetonitrile Strong Bases Condition: With methanol Equilibrate: With water Load Sample: ph of sample should be within * Wash 1: With 25 mm ammonium acetate buffer ph 6.5 Wash 2: With methanol (elutes acidic and neutral analytes) This serves to remove endogenous contaminants from biological sample matrices, and eliminates ion suppression. Dry for 1 minute Elute Analyte: With 2 % formic acid in methanol/acetonitrile (20:80) Depending on analyte solubility and hydrophobicity, isopropanol/methylene chloride (20:80) can be substituted for methanol/acetonitrile Strong Acids Condition: With methanol Equilibrate: With water Load Sample: ph of sample should be 2 ph units above the pk a of the acidic analyte to ensure that the analyte is fully deprotonated Wash 1: With water Wash 2: With methanol (elutes basic and neutral analytes) Elute: 2 % NH 4 OH in methanol * For very hydrophobic compounds methanol can be substituted with methylene chloride and/or isopropanol. * Samples may need to be acidified to disrupt drug-protein interaction. Also sample should be diluted 1:1 with buffer or water ensuring that sample is two ph units below pk a of analyte and two ph units above the pk a of the sorbent. * Modified conditioning step(s) such as acidifying the methanol (2 % formic) may be needed to improve selectivity. The above is a convenient starting point for SPE method development. Further optimization may be required to tailor the method to your specific needs.

8 Strata -X POLYMERC SORBENT SOLD PHASE EXTRACTON strata-x TM Patent Pending REVERSED PHASE Sorbent for CLEANUP of Polar and Non-polar Compounds High recoveries for acidic, neutral and basic compounds ph stability from 1-14 Multiple retention mechanisms for streamlined reversed phase method development Extremely clean extracts LC/MS compatibility Simple method allows for fast sample preparation Material Characteristics Particle Size (µm): 33 Pore Size (Å): 85 Pore Volume (ml/g): 1.2 Surface Area (m 2 /g): 800 Fine Content (<10 µm by Vol. %): <0.2 % Ordering information strata-x Tubes 8B-S-TAK 30 mg/1 ml 8B-S-TBJ 30 mg/3 ml 50 8B-S-UBJ 60 mg/3 ml 50 8B-S-FBJ 200 mg/3 ml 50 8B-S-HBJ 500 mg/3 ml 50 8B-S-ECH mg/6 ml 30 8B-S-FCH 200 mg/6 ml 30 8B-S-HCH 500 mg/6 ml 30 strata-x 96-Well Plates 8E-S-NGB 5 mg/well 2 8E-S-AGB 10 mg/well 2 8E-S-TGB 30 mg/well 2 8E-S-UGB 60 mg/well 2 strata-x On-Line Extraction Cartridge 00M-S033-B0-CB strata-x on-line extraction cartridge, ea 25 µm, 20 x 2.0 mm High Analyte Capacity 33 µm strata-x particles have a very high surface area that results in extremely high analyte capacity. strata-x will retain an analyte mass (including potential interference) that is equivalent to 6-15 % of the sorbent bed mass (i.e., a 200 mg cartridge retains approximately 20 mg of total solute mass). Shorter Dry Down Times The high analyte capacity of strata-x means that you will need a smaller bed mass which results in lower elution volumes for shorter dry down times and fast sample cleanup. Better Selectivity for Polar & Aromatic Compounds CH Cartridge holder, 20 mm ea See p. 15 for Giga tube ordering information Eliminates Well-to-Well Variability Most traditional silica-based sorbents will lose their extraction ability if the sorbent dries or becomes deconditioned. strata-x remains conditioned, yielding extremely high and consistent recoveries, even if the sorbent runs dry! This is a great feature because it eliminates the possibility of well-to-well variability most commonly associated with silica-based 96-well plates. Excellent for 96-Well Plates Phenols from Water 10 0 strata-x SDB % Recovery a: Phenol b: 2,4,6- tribromophenol c: 2-fluorophenol d: 2-chlorophenol % Recovery Doxepin Ranitidine Caffeine Theophylline Procainamide Si-based conventional C e: 2,4,6- trichlorophenol f: 2-nitrophenol g: 2,4- dinitrophenol h: o-cresol Drying time (min.) Conditions: Tubes: 30 mg/1 ml strata-x, mg/1 ml Strata C18-E Samples: Analytes spiked in phosphate buffer saline solution (PBS) at concentration of 1 µg/ ml, n=3 Method: The strata-x method was used. After the conditioning step the sorbent was dried under vacuum from 0 up to 10 min at 10" Hg. Percent recoveries were determined by HPLC analysis. 10

9 Strata -X-C POLYMERC SORBENT Patent Pending Mixed mode Strong cation exchange sorbent for cleanup of basic compounds Enhanced selectivity for aromatic bases Deconditioning resistant surface for consistent sample processing % organic wash allows for complete removal of biological contaminants Material Characteristics Particle Size (µm): 33 Pore Size (Å): 85 Surface Area (m 2 /g): 800 ph Stability: 1-14 onic Capacity: 1 meq/g Reduced on Suppression Ordering information strata-x-c Tubes 8B-S029-TAK 30 mg/1 ml 8B-S029-TBJ 30 mg/3 ml 50 8B-S029-UBJ 60 mg/3 ml 50 8B-S029-FBJ 200 mg/3 ml 50 8B-S029-HBJ 500 mg/3 ml 50 8B-S029-ECH mg/6 ml 30 8B-S029-FCH 200 mg/6 ml 30 8B-S029-HCH 500 mg/6 ml 30 strata-x-c 96-Well Plates 8E-S029-AGB 10 mg/well 2 8E-S029-TGB 30 mg/well 2 8E-S029-UGB 60 mg/well 2 SOLD PHASE EXTRACTON strata-x-c TM See p. 15 for Giga tube ordering information ntens. x Ansys Captiva Protein Precipitation Plate strata-x-c Fast and Flexible Sample Cleanup min on suppression study performed with post column infusion of Atenolol, positive ES mode m/z 267. Contact for additional method details. Captiva Protein precipitation plate is a registered trademark of Varian nc. is in no way affiliated with Varian. May not be representative of all SPE applications shown. Streamlined method development Acrylamide 2 µg/ml acrylamide in 0.2 g French fries TN-007 Single Step Cleanup of Acrylamide from Fried Potatoes Sorbent: strata-x-c 200 mg/3 ml HPLC Column: Synergi 4 μm Polar-RP 150 x 3.0 mm Part No.: 8B-S029-FBJ Part No.: 00F-4336-Y0 Condition: 2 x 1 ml methanol Sample: 10 µl of extract Equilibrate: 2 x 1 ml water Mobile Phase: water / acetonitrile (94:6) Load: 1 ml homogenized supernatant, Flow Rate: 0.4 ml/ min dry for 30 sec Temperature: 25 C Elute: 1 ml water at a flow rate of 0.5 ml/ min Detector: 210 nm Evaporate: remove excess water % Recovery >78 % 84 % >91 % 50 ng/ml 500 ng/ml 2000 ng/ml Acrylamide 11

10 Strata -X-CW POLYMERC SORBENT SOLD PHASE EXTRACTON strata-x-cw TM Mixed mode weak cation exchange Sorbent for cleanup of strong bases Streamlined method development for faster analysis Weak cation exchange mechanism allows for capture and release of quaternary amines π-π, hydrophobic, and polar retention of target analytes Ordering information strata-x-cw Tubes 8B-S035-TAK 30 mg/1 ml 8B-S035-UBJ 60 mg/3 ml 50 8B-S035-FBJ 200 mg/3 ml 50 8B-S035-HBJ 500 mg/3 ml 50 8B-S035-ECH mg/6 ml 30 8B-S035-FCH 200 mg/6 ml 30 8B-S035-HCH 500 mg/6 ml 30 strata-x-cw 96-Well Plates 8E-S035-AGB 10 mg/well 2 See p. 15 for Giga tube ordering information 8E-S035-TGB 30 mg/well 2 8E-S035-UGB 60 mg/well 2 Eliminate Dry Down and Reconstitution Steps! CN-027 Extraction of Clidinium Bromide from Plasma Sorbent: strata-x-cw 30 mg/1 ml Part No.: 8B-S035-TAK Condition: 1 ml methanol Equilibrate: 1 ml water Load: Analyte spiked human plasma (350 µl plasma µl water and acidify with 20 µl phosphoric acid) Wash 1: 1 ml 25 mm sodium acetate buffer ph 4.5, Dry for 1 min Wash 2: 1 ml methanol Elute: 1 ml 5 % formic acid/methanol nject the elution fraction onto the LC/MS (no need to dry down) HPLC Column: Luna 5 µm CN 150 x 2.0 mm Part No.: 00F-4255-B0 Mobile Phase: A: 75 mm ammonium acetate (ph 5.0), B: acetonitrile socratic: A/B (35:65) Flow Rate: 0.2 ml/ min MS nstrument: Bruker Esquire 2000 T Source: ES-Positive mode Sample: Clidinium Bromide (50 ng/ ml) strata-x-cw On-Line Extraction Cartridge 00M-S036-B0-CB strata-x-cw on-line extraction cartridge, ea 20 x 2.0 mm CH Cartridge holder, 20 mm ea No Dry Down Needed A: Standard Solution B: Blank Plasma Extract C: Spiked Plasma Extract % Recovery Clidinium Bromide Selective Elution of Quaternary Amines CN-025 Extraction of Benzalkonium Chloride from Plasma Sorbent: strata-x-cw 30 mg/1 ml Part No.: 8B-S035-TAK Condition: 1 ml methanol Equilibrate: 1 ml water Load: Analyte spiked human plasma (350 µl plasma µl water and acidify with 20 µl phosphoric acid) Wash 1: 1 ml 25 mm sodium acetate buffer ph 4.5, Dry for 1 min Wash 2: 1 ml methanol Elute: 1 ml 5 % formic acid/methanol nject the elution fraction onto the LC-MS (no need to dry down) HPLC Column: Luna 5 µm CN 150 x 2.0 mm Part No.: 00F-4255-B0 Mobile Phase: A: 75 mm ammonium acetate (ph 5.0), B: acetonitrile socratic: A/B (40:60) Flow Rate: 0.2 ml/ min MS nstrument: Bruker Esquire 2000 T Source: ES-Positive mode Samples: C12 Benzalkonium Chloride ( ng/ ml) C14 Benzalkonium Chloride ( ng/ ml) Plasma Extracts (LC/MS extracted ions m/z; 304(C12) and 332(C14)) TC + - All Elute Fraction EC All EC All 0 No Dry Down Needed % Recovery C12 Benzalkonium Chloride C14 Benzalkonium Chloride 12

11 Strata -X-AW POLYMERC SORBENT Mixed mode weak anion exchange Sorbent for cleanup of acids Streamlined method development for faster analysis Selective elution of acidic compounds π-π, hydrophobic, and polar retention of target analytes Selective Elution of Acidic Analytes CN-040 Extraction of Ortho-Phosphotyrosine Sorbent: strata-x-aw 30 mg/1 ml Part No.: 8B-S038-TAK Condition 1: 1 ml methanol Condition 2: 1 ml formic acid / methanol / water (2:25:73) Equilibrate: 1 ml water Load: 1 ml 200 µg/ml in phosphate buffered saline / water (1:3) Wash 1: 1 ml water Wash 2: 1 ml methanol / water (50:50) Elute: 1 ml NH 4 OH / methanol / water (5:50:45) Elution fraction 1:1 with water prior to injection (no need to dry down or reconstitute) HPLC Column: Gemini 5 µm C Å 150 x 4.6 mm Part No.: 00F-4435-E0 Mobile Phase: 20 % acetonitrile / 10 mm KH 2 HPO 4, ph 7.0 / 5 mm TBAC njection: 10 µl Flow Rate: 1.0 ml/min Temperature: 30 C Detection: 220 nm Recovery Data: Sample % Recovery % RSD Ortho-Phosphotyrosine 99 3 mau Ordering information strata-x-aw Tubes 8B-S038-TAK 30 mg/1 ml 8B-S038-UBJ 60 mg/3 ml 50 8B-S038-FBJ 200 mg/3 ml 50 8B-S038-HBJ 500 mg/3 ml 50 8B-S038-ECH mg/6 ml 30 8B-S038-FCH 200 mg/6 ml 30 8B-S038-HCH 500 mg/6 ml 30 strata-x-aw 96-Well Plates 8E-S038-AGB 10 mg/well 2 8E-S038-TGB 30 mg/well 2 8E-S038-UGB 60 mg/well 2 No Dry Down Needed min SOLD PHASE EXTRACTON strata-x-aw TM Efficient Extraction of Nucleotides CN-039 Adenosine Sorbent: strata-x-aw 30 mg/1 ml Part No.: 8B-S038-TAK Condition 1: 1 ml methanol Condition 2: 1 ml formic acid / methanol / water (2:25:73) Equilibrate: 1 ml water Load: 1 ml 0.2 mg/ml in phosphate buffered saline / water (1:1) Wash 1: 1 ml water Wash 2: 1 ml methanol / water (50:50) Elute: 1 ml NH 4 OH / methanol / water (2:25:78) Elution fraction 1:1 with 25 % methanol / 20 mm NH 4 acetate, ph 5.3 prior to injection (no need to dry down or reconstitution) HPLC Column: Gemini 5 µm C Å 150 x 4.6 mm Part No.: 00F-4435-E0 Mobile Phase: 25 % acetonitrile / 10 mm KH 2 HPO 4, ph 7.0 / 5 mm TBAC njection: 5 µl Flow Rate: 1.0 ml/min Temperature: 30 C Detection: 260 nm Recovery Data: Sample % Recovery % RSD 1. 5' AMP ' ADP ' ATP mau No Dry Down Needed min 2 4 See p. 15 for Giga tube ordering information 13

12 Strata SPE Ordering information SOLD PHASE EXTRACTON ORDERNG NFORMATON Strata Tubes Note: Tube sizes not proportional nor actual size. Strata Ordering nformation 30 mg 50 mg mg 60 mg mg 150 mg 200 mg 300 mg 500 mg mg 150 mg 200 mg 500 mg 1 g 1 ml (/box) 3 ml (50/box) 6 ml (30/box) Silica-based sorbents Phase 50 mg mg mg 200 mg 500 mg 500 mg 1 g C18-E 8B-S001-DAK 8B-S001-EAK 8B-S001-EBJ 8B-S001-FBJ 8B-S001-HBJ 8B-S001-HCH 8B-S001-JCH C18-U 8B-S002-EAK 8B-S002-FBJ 8B-S002-HCH C18-T 8B-S004-EAK 8B-S004-FBJ 8B-S004-HBJ 8B-S004-HCH 8B-S004-JCH C8 8B-S005-EAK 8B-S005-FBJ 8B-S005-HBJ 8B-S005-HCH 8B-S005-JCH Phenyl 8B-S006-EAK 8B-S006-FBJ 8B-S006-HBJ 8B-S006-JCH SCX 8B-S010-EAK 8B-S010-EBJ 8B-S010-FBJ 8B-S010-HBJ 8B-S010-HCH 8B-S010-JCH WCX 8B-S027-EAK 8B-S027-FBJ 8B-S027-HBJ 8B-S027-HCH 8B-S027-JCH SAX 8B-S008-EAK 8B-S008-EBJ 8B-S008-FBJ 8B-S008-HBJ 8B-S008-HCH 8B-S008-JCH NH 2 8B-S009-EAK 8B-S009-FBJ 8B-S009-HBJ 8B-S009-HCH 8B-S009-JCH CN 8B-S007-EAK 8B-S007-FBJ 8B-S007-HBJ 8B-S007-HCH 8B-S007-JCH Si-1 8B-S012-EAK 8B-S012-FBJ 8B-S012-HBJ 8B-S012-HCH 8B-S012-JCH Florisil 8B-S013-HBJ 8B-S013-HCH 8B-S013-JCH EPH 8B-S031-HBJ Mixed-mode sorbents (for drugs of abuse) Phase 200 mg 500 mg 200 mg 500 mg 1 g Screen-C GF 8B-S026-FBJ 8B-S026-HBJ 8B-S026-FCH 8B-S026-HCH 8B-S026-JCH Phase mg mg 150 mg 200 mg 300 mg 150 mg 500 mg Screen-C 8B-S016-EAK 8B-S016-EBJ 8B-S016-SBJ 8B-S016-FBJ 8B-S016-RBJ 8B-S016-SCH 8B-S016-HCH Screen-A 8B-S019-EAK 8B-S019-FBJ 8B-S019-HCH Polymeric sorbents Phase mg 200 mg 500 mg 500 mg 1 g SDB-L 8B-S014-EAK 8B-S014-FBJ 8B-S014-HBJ 8B-S014-HCH 8B-S014-JCH Phase 30 mg 60 mg 200 mg 500 mg mg 200 mg 500 mg strata-x 8B-S-TAK 8B-S-UBJ 8B-S-FBJ 8B-S-HBJ 8B-S-ECH 8B-S-FCH 8B-S-HCH strata-x-c 8B-S029-TAK 8B-S029-UBJ 8B-S029-FBJ 8B-S029-HBJ 8B-S029-ECH 8B-S029-FCH 8B-S029-HCH strata-x-cw 8B-S035-TAK 8B-S035-UBJ 8B-S035-FBJ 8B-S035-HBJ 8B-S035-ECH 8B-S035-FCH 8B-S035-HCH strata-x-aw 8B-S038-TAK 8B-S038-UBJ 8B-S038-FBJ 8B-S038-HBJ 8B-S038-ECH 8B-S038-FCH 8B-S038-HCH f Strata SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE product for FREE! See p. 16 for 96-well plate ordering information 14

13 Strata GGA TUBES Pre-packed formats eliminate excess labor associated with glass packed flash columns High capacity formats available in 12, 20, 60, and 150 ml tube sizes Narrow particle distribution provides consistent and even flow Great for purification of organic compounds Recommended Capacity and Elution Volumes for Strata Giga Tubes Silica-Based Sorbents C18, C8, Phenyl, SCX, SAX, NH 2, CN, Silica(Si-1), FL-PR (Florisil ), ABW, EPH f Strata SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE product for FREE! Note: Tube sizes not proportional nor actual size. Polymeric Sorbents strata-x, strata-x-c, strata-x-cw, strata-x-aw SOLD PHASE EXTRACTON Strata Giga Tubes 00 mg 2 g 5 g 10 g 20 g 12 ml 12 ml 20 ml 60 ml 60 ml (20/box) (20/box) (20/box) (16/box) (16/box) Retention Capacity 25 mg mg 250 mg 500 mg 0 mg Suggested Elution Volume 6 ml 24 ml 60 ml 120 ml 240 ml 500 mg 1 g 1 g 12 ml 12 ml 20 ml (20/box) (20/box) (20/box) 75 mg 150 mg 150 mg 10 ml 20 ml 20 ml Ordering nformation Strata Giga Tubes 12 ml (20/box) 20 ml (20/box) 60 ml (16/box) 150 ml (8/box) Phase 500 mg 1 g 2 g 1 g 5 g 10 g 20 g 50 g 70 g C18-E 8B-S001-HDG 8B-S001-KDG 8B-S001-LEG 8B-S001-MFF 8B-S001-VFF 8B-S001-YSN 8B-S001-ZSN C8 8B-S005-KDG 8B-S005-LEG 8B-S005-MFF Phenyl 8B-S006-KDG SCX 8B-S010-KDG 8B-S010-LEG 8B-S010-MFF 8B-S010-VFF SAX 8B-S008-HDG 8B-S008-KDG 8B-S008-LEG 8B-S008-MFF 8B-S008-VFF NH 2 8B-S009-HDG 8B-S009-KDG 8B-S009-LEG 8B-S009-MFF 8B-S009-VFF CN 8B-S007-KDG 8B-S007-LEG Si-1** 8B-S012-HDG 8B-S012-KDG 8B-S012-LEG 8B-S012-MFF 8B-S012-VFF 8B-S012-YSN 8B-S012-ZSN FL-PR (Florisil ) 8B-S013-KDG 8B-S013-LEG 8B-S013-MFF strata-x 8B-S-HDG 8B-S-JDG 8B-S-JEG strata-x-c 8B-S029-HDG 8B-S029-JDG strata-x-cw 8B-S035-HDG 8B-S035-JDG 8B-S035-JEG strata-x-aw 8B-S038-HDG 8B-S038-JDG strata ABW 8B-S030-KDG 8B-S030-LEG 8B-S030-MFF strata EPH** 8B-S031-LEG See p. 22 for Giga Tube & Vacuum Manifold Accessories Retention capacities and elution volumes are specific to the chemical nature of the analyte being extracted, its concentration in the sample, the chemical nature of the eluting solvent and the bed mass used. The above are only guidelines. An elution study should be conducted. As a general rule, recommended solvent wash and elution volumes for silica-based material should be 1.2 ml per mg of sorbent and 2 ml per mg of polymeric sorbent, ** Teflon-coated tubes available, contact for details. 15

14 SOLD PHASE EXTRACTON Strata High-Throughput Plates Strata hgh-throughput 96-Well Plates Up to 2 ml sample volume per well Compatible with most automated systems including: Zymark SciCLONE TomTec Quadra-96 Beckman Coulter Biomek 2000 Gilson 215 ORDERNG NFORMATON (2 PLATES/box) 2 Plates/Box 96-Well Plates Phase 5 mg 10 mg 25 mg 30 mg 50 mg 60 mg mg strata-x-c 8E-S029-AGB 8E-S029-TGB 8E-S029-UGB strata-x-cw 8E-S035-AGB 8E-S035-TGB 8E-S035-UGB strata-x-aw 8E-S038-AGB 8E-S038-TGB 8E-S038-UGB f Strata SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE product for FREE! strata-x 8E-S-NGB 8E-S-AGB 8E-S-TGB 8E-S-UGB SDB-L 8E-S014-DGB Screen-C 8E-S016-DGB Screen-A 8E-S019-CGB 8E-S019-DGB C18-E 8E-S001-CGB 8E-S001-DGB 8E-S001-EGB C18-U 8E-S002-DGB C8 8E-S005-CGB 8E-S005-DGB 8E-S005-EGB Phenyl 8E-S006-DGB 8E-S006-EGB NH 2 8E-S009-CGB 8E-S009-DGB CN 8E-S007-CGB 8E-S007-DGB WCX 8E-S027-CGB 8E-S027-DGB 8E-S027-EGB SCX 8E-S010-CGB 8E-S010-EGB SAX 8E-S008-CGB 8E-S008-DGB 8E-S008-EGB Si-1 8E-S012-DGB Strata mpact Protein Precipitation Plates Solvent Shielding Technology for clean, leak-free results Versatile formats for fast sample processing Strata Method Development 96-Well Plate for Rapid Screening Each Strata 96-well plate contains six silica-based sorbents and two polymeric resins. One row of C18-E, C18-U, C18-M, C18-T, C8, PH (50 mg/well) and strata-x and SDB-L (30 mg/well) for parallel screening ORDERNG NFORMATON (2 PLATES/box) Strata mpact Precipitation Plates Part No. Description Unit/Box Price CE Strata mpact Square Well Plate, 2 ml 2 ORDERNG NFORMATON Strata Method Development 96-Well Plate Part No. Unit/Box Price KS ea See p. 8 for Strata-X Method Development Kits See p. 8 for Strata Starter Kit for Protein Precipitation Contact or your local distributor for latest 96-well plate technical note. 16

15 Strata hgh-throughput Accessories 96-Well Plate & Protein Precipitation Plate Accessories Sealing Mats and Collection Plates Preserves sample integrity Maximizes sample recovery No adhesives which can cause interference Made of chemically resistant polypropylene ORDERNG NFORMATON Sealing Mats* Part No. Description Unit Price AH Pierceable sealing mats, 96 square well 50/pk AH Sealing tape pad 10/pk Collection Plates* Part No. Description Unit Price AH ml/well 96 square well collection plate 50/pk AH ml/well 96 square well collection plate 50/pk AH ml/well 96 square well collection plate 50/pk AH ml/well 96 round well collection plate 50/pk AHO-7408 Solvent waste reservoir tray 25/pk Manifold Made of durable acrylic Fits Strata collection plates (0.35, 1 and 2 ml) Affordable On-Line Format Strata on-line extraction cartridges Extremely rapid method development Elimination of storage Reduced sample preparation time Long column lifetimes FLOW ORDERNG NFORMATON strata On-Line Extraction Cartridge 00M-S033-B0-CB strata-x on-line extraction cartridge, ea 20 x 2.0 mm 00M-S036-B0-CB strata-x-cw on-line extraction cartridge, ea 20 x 2.0 mm 00M-S039-B0-CB Strata C18 on-line extraction cartridge, ea 20 x 2.0 mm 00M-S101-B0-CB Strata C8 on-line extraction cartridge, ea 20 x 2.0 mm CH Cartridge holder, 20 mm ea Solid-Phase Extraction: Principles and Practice By E.M. Thurman. 1998, 352pp Part No.: AA Price: SOLD PHASE EXTRACTON Strata High-Throughput Accessories & On-Line Cartridges ORDERNG NFORMATON Manifold* Part No. Description Unit Price AH Well Plate Manifold, Acrylic 1 ea Handbook of Bioanalysis and Drug Metabolism Edited by Gary Evans Part No.: AA Price: Replacement Parts Part No. Description Unit Price AH Well Plate Manifold replacement gasket, profile 1 ea (to fit between top plate and vacuum chamber) AH Well Plate Manifold replacement gasket, flat 1 ea (to fit between lid and plate) *Manifold, Collection plates, & Sealing mats compatible with 2 ml mpact plate and strata 96-well plate formats only. Contact or your local distributor for Free SPE technical notes. 17

16 Strata -X APPLCATONS SOLD PHASE EXTRACTON Strata Applications Extremely Clean Extracts CN-008 Extraction of Polar Steroids from Serum Sorbent: Part No.: Condition: Equilibrate: Load: Wash: Elute: Evaporate: strata-x 30 mg/1 ml Detector: 8B-S-TAK Peaks: 1 ml methanol 1 ml water 1 ml porcine spiked with analyte 1 ml 5 % methanol in water 1 ml methanol add 25 µl of 0.1 mg/ml butyl paraben (external standard) dry down under slow stream of N 2 and reconstitute in 200 µl acetonitrile 254 nm 1. Prednisolone 2. Betamethasone 3. Butyl Paraben (external std) 4. Betamethasone Valerate Chromatogram of Extracts: A) Blank B) Spiked Sample 1 3 Reversed Phase HPLC Column: Synergi Max-RP 4 µm 150 x 4.6 mm SecurityGuard C18 4 x 3.0 mm Part No.: 00F-4337-E0 and AJ Sample: 50 µl of reconstituted extract Mobile Phase: A: 20 mm KH 2 PO 4 (ph 7.0) B: methanol A/B (35:65) for 20 min Flow Rate: 1.0 ml/min Temperature: 25 C 2 4 B A High Recoveries for Drugs of Abuse CN-014 Methadone from Urine Sorbent: Part No.: Condition: Equilibrate: Load: strata-x-c 60 mg/3 ml 8B-S029-UBJ 2 ml methanol 2 ml water 3 ml urine spiked with analytes and S (0.5 µg/ml); dilute 1:1 with mm KH 2 PO 4, ph 6; acidified with 2 % phosphoric acid 2 ml 0.1 N HC 2 ml methanol 2 ml 5 % NH4OH/methanol Wash 1: Wash 2: Elute: Evaporate: dry down and reconstitute with 200 µl mobile phase HPLC Column: Synergi Max-RP 4 μm 150 x 4.6 mm Part No.: 00F-4337-E0 Sample: 50 µl of reconstituted urine extract Mobile Phase: A: 0.1 % TFA in water, ph 2.1 B: acetonitrite A/B (65:35) Flow Rate: 1.0 ml/min Temperature: 25 C 1 % organic wash results in clean extracts Detector: Peaks: Chromatogram of Extracts: 210 nm 1. Estazolam (S) 2. Methadone A) Blank B) Spiked Sample 2 Mixed Mode Cation Exchange B A min % Recovery % Methadone CN-005 Barbiturates from Urine Sorbent: Part No.: Condition: Equilibrate: Load: Wash 1: Wash 2: Elute: Evaporate: strata-x-c 60 mg/3 ml 8B-S029-UBJ 2 ml methanol 2 ml water 2 ml human urine spiked with analytes and S (0.5 µg/ml); dilute 1:1 with water 2 ml 5 % methanol in water 2 ml 10 % methanol in 2 % acetic acid and dry for 1 min 2 ml 35 % methanol/2 % NH 4 OH dry down under slow stream of N 2 and reconstitute in 300 µl mobile phase HPLC Column: Synergi Max-RP 4 μm 150 x 4.6 mm Part No.: 00F-4337-E0 Sample: 50 µl of reconstituted urine extract Mobile Phase: A: 20 mm KH 2 PO 4, ph 7.0 B: acetonitrile Gradient: A/B: (80:20) hold 3 min to (50:50) in 12 min Flow Rate: 1.0 ml/min Temperature: 25 C High recoveries by strong cation exchange retention mechanism Detector: 214 nm Peaks: 1. Phenobarbital 3. Amobarbital 2. Butalbital 4. Secobarbital (S) Chromatogram of Extracts: A) Blank B) Spiked Sample 1 2 Mixed Mode Cation Exchange 3 4 B A min % Recovery % 103% % Phenobarbital Butalbital Amobarbital 18

17 Strata EPH Applications Extractable Petroleum Hydrocarbons (EPH) Fractionation Method High Recoveries for Extractable Petroleum Hydrocarbons (50ppm) C 9 -C 18 and C 19 -C 36 C 11 -C 22 Aliphatic Hydrocarbons Aromatic Hydrocarbons Compound % Recovery % RSD (N=3) n-nonane (C 9 ) n-decane (C 10 ) n-dodecane (C 12 ) n-tetradecane (C 14 ) n-hexadecane (C 16 ) n-octadecane (C 18 ) n-nonadecane (C 19 ) n-eicosane (C 20 ) n-docosane (C 22 ) n-tetracosane (C 24 ) n-hexacosane (C 26 ) n-octacosane (C 28 ) n-triacotane (C 30 ) n-hexatriacontane (C 36 ) Surrogate 46.1 Compound % Recovery % RSD (N=3) Naphthalene Methylnaphthalene Acenaphthalene Acenaphthene Flourene Phenanthrene Anthracene Fluoranthene Pyrene Benz[a]anthracene Chrysene Benzo[b]fluoranthene Benzo[k]fluoranthene Benzo[a]pyrene ndeno[1,2,3-cd]pryrene Dibenz[a,h]anthracene Benzo[g,h,i]perylene Surrogate 90.5 SOLD PHASE EXTRACTON Strata Applications Sample Preparation: Sorbent: Part No.: Equilibrate: Load: Elute Aliphatics C 9 -C 18 and C 19 -C 36 : Elute Aromatics C 11 -C 22 : Concentrate: Solid Phase Extraction Conditions Strata EPH 5 g/20 ml 8B-S031-LEG 30 ml Hexane Sample 11 ml Hexane (Lower elution volume allows for more concentrated fraction and faster sample dry down times) 20 ml Methylene Chloride Samples to 1 ml min Peak Compound Ret. Time % RSD 1 C C C C Butylhydroxytoluene C C C C a-Androstane (S) Chloro-Octadecane (surr) C C C C C C GC Analysis Conditions GC Column: Zebron ZB-5ms Part No.: 7HM-G Dimensions: 30 meter x 0.32 mm x 0.25 µm njection: 2 µl 285 C Carrier Gas: 3 ml/min Oven Program: 60 C for 1 minute then ramp to 290 C at 8 C/min hold for 6.75 minutes Detector: 315 C min Peak Compound Ret. Time % RSD 1 Naphthalene Methylnaphthalene Fluorobiphenyl (Frac Surr) Acenaphthalene Bromonaphthalene (Frac Sur) Acenaphthene Phthalate Fluorene Phenanthrene Anthracene o-terphenyl (Surr) a-Androstane Fluoranthene Chloro-Octadecane (surr-aliphatic) Pyrene Benz[a]anthracene Chrysene Benzo[b]fluoranthene Benzo[k]fluoranthene Benzo[a]pyrene ndeno[1,2,3-cd]pryene Dibenz[a,h]anthracene Benzo[g,h,i]perylene

18 SOLD PHASE EXTRACTON Sepra Bulk Sorbents Sepra BULK SORBENTS Available in polar and non-polar phases for wide range of selectivities A good alternative to Varian Bondesil and SOLUTE bulk sorbents Available in gram to multi-kilogram quantities Economical Rugged Sepra high performance bulk SPE sorbents are excellent for applications such as MSPD (matrix solid phase dispersion) in which drugs of interest are separated from homogenized animal tissue or food sources. These high quality sorbents can also be used for flash analysis to effectively purify organic compounds. f Sepra Bulk products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Sepra Bulk for FREE! Material Characteristics Particle Size Pore Size Surface Area Carbon Load Bonding End Capping ph Stability onic Capacity Phase (µm) (Å) (m2 /g) (%) (meq/g) Reversed Phase C18-E trifunctional Yes 1 to 8 C18-T trifunctional Yes 1 to 8 C trifunctional Yes 1 to 8 Phenyl trifunctional Yes 1 to 8 CN trifunctional No 1 to 8 SDB-L No 1 to 14 Normal Phase Silica No 1 to 8 FL-PR (Florisil Pesticide Residue) No 1 to 8 on Exchange SCX trifunctional No 1 to SAX trifunctional No 1 to NH trifunctional No 1 to Ordering nformation Sepra Bulk Sorbents Phase g Price 1kg Price Reversed Phase C18-E 04G K-4348 C18-T 04G K-4405 C8 04G K-4406 Phenyl 04G K-4407 CN 04G K-4409 SDB-L 04G K-4412 Normal Phase Silica 04G K-4410 FL-PR 04G K-4411 (Florisil Pesticide Residue) on Exchange SCX 04G K-4413 SAX 04G K-4414 NH 2 04G K-4408 nterested in MSPD for your analysis? Please contact us for technique and accessory information. Bondesil is a registered trademark of Varian nc. SOLUTE is a registered trademark of nternational Sorbent Technology Ltd. Florisil is a registered trademark of U.S. Silica 20

19 3M empore Disks for Environmental Analysis Ordering nformation Empore Extraction Disks Part No. Mfr. No. Description Disk Diameter (mm) Unit Price AH C /pk AH C /pk AH C /pk AH Anion-Exchange 47 20/pk AH SDB-XC 47 20/pk AH SDB-RPS 47 20/pk AH Oil and Grease 47 20/pk Empore Disk Cartridges Part No. Mfr. No. Description Size* Unit Price AH C18-SD** 4 mm/1 ml /pk AH C18-SD*a* 7 mm/3 ml 50/pk AH C18-SD** 10 mm/6 ml 30/pk For fast and easy sample extraction, offers filtration glassware for Empore Extraction Disks. Our 47 mm all-glass microfiltration units are used for individual extractions and are described on page 350. Three- and six-station Extraction Manifolds are also available, please inquire. SOLD PHASE EXTRACTON 3M Empore * Disk diameter/cartridge volume. ** SD= Standard Density is optimized for biological matrices (40 µm particle, enhanced flow characteristics). Filtration Glassware Part No. Description Disk Diameter (mm) Unit Price Filtration Glassware Complete Assemblies AH FilterSys, Filtration Glassware, All-Glass, 300 ml funnel with 1 L flask 47 ea AH FilterSys, Filtration Glassware, All-Glass, 500 ml funnel with 2 L flask 47 ea AH FilterSys, Filtration Glassware, All-Glass,0 ml funnel with 4 L flask 47 ea Filtration Glassware Component Parts AH Fritted Support Base, 40/35 Ground-Glass Joint 47 ea AH Funnel Reservoir, Graduated, 300 ml 47 ea AH Funnel, graduated 500 ml 47 ea AH Funnel, graduated 0 ml 47 ea AH Vacuum Filter Flask, 1 L, 40/35 Ground-Glass Joint 47 ea AH Aluminum Clamp 47 ea Ordering nformation Disk Plates for Silica-Based Extraction Part No. Mfr. No. Description Unit Price Standard Well Extraction Disk Plates, 1.2 ml Capacity CE (SD) C2-SD (Ethyl) ea CE (SD) C8-SD (Octyl) ea CE (SD) C18-SD (Octadecyl) ea CE (SD) MPC-SD (Mixed Phase Cation) ea Deep Well Extraction Disk Plates, 2.5 ml Capacity CE (SD) C18-SD (Octadecyl) ea Universal Resin Membrane Extraction Disk Plates Part No. Mfr. No. Description Unit Price CE (SD) Universal Resin Plate, ea Standard 1.2 ml Capacity CE (SD) Universal Resin Plate, ea Deep Well 2.5 ml Capacity Protein Precipitation /Filter Plate Part No. Mfr. No. Description Unit Price Filter Plate/Precipitation Plates CE Filter Plate PPT, ea Standard 1.2 ml Capacity CE Filter Plate PPT, ea Deep Well 2.5 ml Capacity See p. 16 for Strata mpact Protein Precipitation Plates. All 3M Filtration Products are available from. Call for availability. * 3M and Empore are trademarks of 3M. 21

20 SPE vacuum manifolds SOLD PHASE EXTRACTON Vacuum Manifolds Multiple sample processing saves time and money Female Luer inlets fit all male Luer tipped SPE tubes and cartridges nert Teflon and polypropylene flow path Glass chamber allows easy visual monitoring Adjustable racks accommodate a variety of test tubes, vials and flasks ORDERNG NFORMATON 10 - Position Tall-Boy Vacuum Manifold* Part No. Description Unit Price AH SPE 10-Position Tall-Boy Vacuum Manifold, complete assembly ea AH SPE 10-Position Tall-Boy Vacuum Manifold, Glass Chamber ea AH SPE 10-Position Tall-Boy Vacuum Manifold, Cover, Gasket and 10 Stopcocks ea 12 Position Vacuum Manifold* AH SPE 12-Position Vacuum Manifold Set, complete assembly ea 12 Position Vacuum Manifold Replacement Parts AH SPE 12-Position Glass Chamber ea AH SPE Cover, Gasket and 12 Stopcocks ea AH SPE Gaskets 2/pk AH SPE Vacuum Gauge, Valve and Glass Chamber ea AH SPE Collection Rack, including shelves, legs, clips and posts ea AH SPE Plate for 13 mm Test Tube ea AH SPE Plate for 16 mm Test Tube ea AH SPE Plate for Volumetric Flask ea AH SPE Plate for Autosampler Vial ea AH SPE Plate, Dimple ea AH SPE Plate, Base ea AH SPE Stopcocks 12/pk AH SPE 12-Position Vacuum Waste Container, polypropylene 10/pk 24 Position Vacuum Manifold* AH SPE 24-Position Vacuum Manifold Set, complete assembly ea 24 Position Vacuum Manifold Replacement Parts AH SPE Glass Chamber ea AH SPE Cover, Gasket and 24 Stopcocks ea AH SPE Gaskets 2/pk AH SPE Vacuum Gauge ea AH SPE Collection Rack, including shelves, legs, clips and posts ea AH SPE Plate for 13 mm Test Tube ea AH SPE Plate for 16 mm Test Tube ea AH SPE Stopcocks 24/pk General Vacuum Manifold Accessories AH SPE Manifold Needles, polypropylene 12/pk AH SPE Manifold Needles, polypropylene 24/pk AH SPE Manifold Needles, stainless steel 12/pk AH SPE Manifold Needles, stainless steel 24/pk AH SPE Drying Attachment for 12-Position manifold ea AH SPE Drying Attachment for 24-Position manifold ea AH Female Luer Fittings 2/pk AH Male Luer Fittings 2/pk AH Support Posts for Rack 3/pk AH Legs for Cover, black 4/pk AH Vacuum Gauge and Valve Assembly ea AH Valve Assembly Only ea AH Vacuum Gauge Only ea AH Retaining Clips 12/pk AH Vacuum Manifold Plugs 50/pk AH Control Valve, Teflon 25/pk AH Control Valve, Teflon 50/pk AH Teflon Needles /pk AH Teflon Needles 500/pk AH Adaptor Caps for 1, 3 and 6 ml SPE tubes, polyethylene, with Luer hole in the top 15/pk AH Adaptor Caps for 12 and 20 ml SPE tubes, polyethylene, with Luer hole in the top 5/pk AH Adaptor Caps for 60 ml SPE tubes, polyethylene, with Luer hole in the top 5/pk AH Adaptor Caps for 150 ml SPE tubes, polyethylene, with Luer hole in the top ea Have Large Sample Volume but Need a Small Bed Mass? Use an adaptor cap to attach another SPE tube, which can be used to increase the reservoir size for washing or eluting solvents. See p. 16 for Strata mpact Precipitation Plates. Adaptor Cap (AH0-7191) * Manifolds include: Vacuum-tight glass chamber, polypropylene lid with gasket, bleed valve and gauge, stopcock valves, collections racks, polypropylene needles (1) The 10-position Tall Boy Vacuum Manifold Collection Rack includes 4 plates: one base plate, one dimple plate, one small plate and one large plate and three riser bar legs, along with 12 manifold clips to support the plates. The assembly also includes 10 polypropylene needles, 10 stopcocks and 4 black legs to support the lid when taken off the glass block. (2) The 12-position Collection Rack consists of 3 support posts, bottom plate, 13 mm plate, 16 mm plate, autosampler plate, volumetric plate, and 12 retaining clips. (3) The 24-position Collection Rack consists of 3 support posts, bottom plate, dimple plate, 13 mm plate, 16 mm plate, and 24 retaining clips. 22