Without the Dry Down. Successful Bioanalytical Sample Preparation. Microelution 96-Well SPE Plates

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1 Polymeric SPE Successful Bioanalytical Sample Preparation Without the Dry Down Microelution 96-Well SPE Plates Increase sensitivity Process small or limited sample volumes Save time and reduce costs Reduce loss of challenging analytes

2 Simple Steps for Clean Samples Solid Phase Extraction (SPE is an effective technique for cleaning up and concentrating samples prior to UHPLC/ HPLC or GC analysis. Available in several chemistries, the SPE sorbent selectively targets your analytes of interest, allowing interferences and impurities to be washed away by following easy steps. Solid Phase Extraction (SPE Basics Step 1. Condition A conditioning step activates the SPE sorbent Step 2. An equilibration solvent prepares the sorbent for interaction with your sample Equilibrate Step 3. Target analytes are selectively targeted and bound to the SPE sorbent Load Sample Interferences Target Analytes Step 4. Wash Away Impurities Interferences are washed away Interferences Step. Collect Analytes of Interest An elution solvent breaks the bond between the SPE sorbent and your target analytes, allowing them to be collected for further analysis Your sample is now clean and concentrated Target Analytes SPE Is Easy! Turn to page 4 to learn how Strata -X Microelution 96-Well SPE Plates can further improve your work Phenomenex, Inc. All rights reserved. 2 Phenomenex l WEB:

3 Microelution SPE is Faster and More Sensitive than Traditional SPE pp. 4- Skip the Dry Down Step m Increase Throughput p. 6 m Reduce the Risk of Analyte Loss p. 7 Easily Process Samples p. 8 Applications: m Reliable Peptide Analysis p. 9 m Ultra Sensitive Pharmaceutical Analysis p. 10 m Reproducible Clinical Research Analysis p. 11 m Rapid Analysis of Drugs of Abuse p. 12 Method Development / Web Tools / Seminars p. 13 rdering Information pp

4 Microelution SPE is Faster and More Sensitive Traditional 10 mg SPE 200 µl Step 1. Condition 10 mg 200 µl Step 2. Equilibrate 10 mg 100 µl Not amenable to small or limited sample volumes Step 3. Load Sample 10 mg µl Step 4. Wash 10 mg 10 mg Concentration of target analytes is diluted Step. Elute 200 µl Dry down is required to increase sensitivity, adding time and cost Step 6. ~30-60 EXTRA minutes Thermolabile or unstable analytes can be lost during this step 4 Phenomenex l WEB: Dry Down and Reconstitute

5 Strata-X Microelution SPE Step Condition 200 µl Step Equilibrate 200 µl Step 3. Step 3. Load Load Sample Sample sample volumes as low as 10 µl Process small or limited sample volumes without constraints Step 4. Step 4. Wash Away Wash Impurities µl Step. Elute At least 8x more sensitive than traditional 10 mg SPE! 2 µl Step 6. Dry Down and Reconstitute NT REQUIRED Save 30 or more minutes per 96-well plate! Save on labor costs, do more in less time

6 Increase Throughput Save 30 or more minutes per 96-well plate by eliminating timely dry down and reconstitution steps. Traditional 10 mg SPE Strata -X Microelution SPE 60 Minutes Total Processing Time 30 Minutes No Dry Down! 768 Samples Throughput (in 8 hours 1,36 Samples DUBLE Your Throughput REDUCE Cost per Sample! Visit to learn more! 6

Reduce Analyte Loss Many target analytes such as peptides and thermolabile compounds can be lost during dry down steps. Stop risking analyte loss and skip the dry down step without losing sensitivity.

(200 µl serum diluted 1:1 with 4 % Phosphoric acid in water 400 µl diluted serum (200 µl serum diluted 1:1 with 4 % Phosphoric acid in water Wash 1 400 µl 2 % Formic acid in water 200 µl 2 % Formic

7 Reduce Analyte Loss Many target analytes such as peptides and thermolabile compounds can be lost during dry down steps. Stop risking analyte loss and skip the dry down step without losing sensitivity. Strata-X 96-Well SPE Plate, 10 mg/well Strata-X Microelution 96-Well SPE Plate, 2 mg/well Condition 400 µl Methanol 200 µl Methanol Equilibrate 400 µl Water 200 µl Water Load 400 µl diluted serum (200 µl serum diluted 1:1 with 4 % Phosphoric acid in water 400 µl diluted serum (200 µl serum diluted 1:1 with 4 % Phosphoric acid in water Wash µl 2 % Formic acid in water 200 µl 2 % Formic acid in water Wash µl 20 % Acetonitrile in water 200 µl 20 % Acetonitrile in water Elute Dry Down 2x 17 µl Trifluoroacetic acid/acetonitrile/water (1:74:2 Dry down under a gentle stream of Nitrogen and reconstitute in 0 µl Trifluoroacetic acid/ Acetonitrile/Water (1:74:2 Inject 10 µl 10 µl 2x 2 µl Trifluoroacetic acid/acetonitrile/water (1:74:2 NT REQUIRED Intensity, cps 1.6e 1.e 1.4e 1.3e 1.2e 1.1e 1.0e 9.0e4 8.0e4 7.0e4 6.0e4.0e4 4.0e4 3.0e4 2.0e4 1.0e4 Column: Kinetex µm C8 Dimensions: 0 x 4.6 mm Part No.: 00B-4608-E0 Mobile Phase: A: 0.1 % Formic acid in Water B: 0.1 % Formic acid in Methanol Gradient: Time (min % B Flow Rate: 0.6 ml/min Temperature: Ambient Detection: MS/MS, Triple Quad 400 (SCIEX, ESI Sample: DALDA C8 (peptide, spiked at 10 ng/ml Microelution SPE Skip the dry down and increase recovery Absolute Recovery: 9 % % CV (N6:.7 10 mg SPE ~0 % loss is observed after dry down step Absolute Recovery: 0 % % CV (N6: 10. App ID and min 1. CNS Delivery and Pharmacokinetic Evaluations of DALDA Analgesics Peptide Analog Administered in Nano-Sized il-in-water Emulsion Formation. Shah L., Gattacceca F. and Amiji MM. Pharm Res ( : Analgesic Efficacy and Safety of DALDA Peptide Analog Delivery to the Brain using il-in-water Nanoemulsion Formation. Shah L., Kulkarni P., Ferris C., and Amiji MM. Pharm Res. ( :

Easily Process Samples Strata -X Microelution 96-well SPE plates are compatible with standard vacuum manifolds, positive pressure manifolds, and liquid handling systems, making it even easier to

8 Easily Process Samples Strata -X Microelution 96-well SPE plates are compatible with standard vacuum manifolds, positive pressure manifolds, and liquid handling systems, making it even easier to process your samples. Cost Effective Benchtop Processing 96-Well Plate Vacuum Manifold Part No. AH0-890 Presston 100 Positive Pressure Manifold Part No. AH High-Throughput, Hands-Free Liquid Handling Systems Use the dimensions outlined below to program your liquid handling system measured in mm 8

9 Applications Reliable Peptide Analysis Strata-X-CW Microelution 96-Well SPE Plate, 2 mg/well Part No. 8M-S03-4GA Condition 200 µl Methanol Equilibrate 200 µl Water Load 400 µl diluted serum (200 µl serum diluted 1:1 with 4 % Phosphoric acid in water Wash µl % Ammonium hydroxide in water Wash µl 20 % Acetonitrile in water Elute 2x 2 µl Trifluoroacetic Acid/Acetonitrile/Water (1:74:2 Inject 2 µl 2299 Intensity, cps 9.0e4 8.e4 8.0e4 7.e4 7.0e4 6.e4 6.0e4.e4.0e4 4.e4 4.0e4 3.e4 3.0e4 2.e4 2.0e4 1.e4 1.0e Column: Aeris WIDEPRE 3.6 µm XB-C18 Dimensions: 0 x 2.1 mm Part No.: 00B-4482-AN Mobile Phase: A: 0.1 % Formic acid in Water B: 0.1 % Formic acid in Methanol Gradient: Time (min % B Flow Rate: 0.6 ml/min Temperature: Ambient Detection: MS/MS, Triple Quad 400 (SCIEX, ESI min App ID 2299 Absolute Recovery of Goserelin at ng/ml Peptide RT (min % Absolute Recovery % CV (N10 Goserelin Phenomenex l WEB: 9

Applications Ultra Sensitive Pharmaceutical Analysis Strata -X-C 96-Well SPE Plate, 10 mg/well Condition 00 µl Methanol 200 µl Methanol Equilibrate 00 µl Water 200 µl Water Load 70 µl diluted serum

00 µl 2 % Formic acid in water 200 µl 2 % Formic acid in water Wash 2 00 µl Methanol 200 µl Methanol Elute 37 µl (3x 12 µl % Ammonium hydroxide in Acetonitrile/Methanol (60:40 Inject 1 µl 1 µl 2 µl %

10 Applications Ultra Sensitive Pharmaceutical Analysis Strata -X-C 96-Well SPE Plate, 10 mg/well Condition 00 µl Methanol 200 µl Methanol Equilibrate 00 µl Water 200 µl Water Load 70 µl diluted serum (37 µl serum diluted 1:1 with 4 % Phosphoric acid in water Strata-X-C Microelution 96-Well SPE Plate, 2 mg/well 70 µl diluted serum (37 µl serum diluted 1:1 with 4 % Phosphoric acid in water Wash 1 00 µl 2 % Formic acid in water 200 µl 2 % Formic acid in water Wash 2 00 µl Methanol 200 µl Methanol Elute 37 µl (3x 12 µl % Ammonium hydroxide in Acetonitrile/Methanol (60:40 Inject 1 µl 1 µl 2 µl % Ammonium hydroxide in Acetonitrile/ Methanol (60: e.0e 4.e 4.0e 3.e Intensity, Intensity, cps cps 3.0e 2.e 2.0e 1.e 1.0e.0e4 Timolol (1 ng/ml Microelution SPE 1 More Concentrated! 10 mg SPE Column: Kinetex 2.6 µm Biphenyl Dimensions: 0 x 2.1 mm Part No.: 00B-4622-AN Mobile Phase: A: 0.1 % Formic acid in Water B: 0.1 % Formic acid in Acetonitrile Gradient: Time (min % B Flow Rate: 0. ml/min Temperature: Ambient Detection: MS/MS, API 000 (SCIEX 9 9 App ID and min min Intensity, cps 9.e 9.0e 8.e 8.0e 7.e 7.0e 6.e 6.0e.e.0e 4.e 4.0e 3.e 3.0e 2.e 2.0e 1.e 1.0e.0e4 0.0 Procaine (1 ng/ml 10 mg SPE Microelution SPE 1 More Concentrated! min App ID and

11 Applications Reproducible Clinical Research Analysis Strata-X-C Microelution 96-Well SPE Plate, 2 mg/well Part No. 8M-S029-4GA Condition 200 µl Methanol Equilibrate 200 µl Water Load 400 µl diluted plasma (200 µl plasma diluted 1:1 with 4 % Phosphoric acid in water Wash µl 2 % Formic acid in water Wash µl Methanol Elute 2x 2 µl % Ammonium hydroxide in Acetonitrile/ Methanol (60:40 Inject 2 µl Intensity, cps 1.6e6 1.e6 1.4e6 1.3e6 1.2e6 1.1e6 1.0e6 9.0e 8.0e 7.0e 6.0e.0e 4.0e 3.0e 2.0e 1.0e min Column: Kinetex 2.6 µm Biphenyl Dimensions: 0 x 2.1 mm Part No.: 00B-4622-AN Mobile Phase: A: 0.1 % Formic acid in Water B: 0.1 % Formic acid in Methanol Gradient: Time (min % B Flow Rate: 0. ml/min Temperature: Ambient Detection: MS/MS, Triple Quad 400 (SCIEX App ID Absolute Recovery of 7 Antipsychotics at 2 ng/ml Antipsychotics RT (min % Absolute Recovery Recovery % CV (N4 lazepine Norclozapine Clozapine H Risperidone Risperidone Norquetiapine Quetiapine

12 Applications Rapid Analysis of Drugs of Abuse Strata -X-C Microelution 96-Well SPE Plate, 2 mg/well Part No. 8M-S029-4GA Condition 200 µl Methanol Equilibrate 200 µl Water Load 400 µl diluted urine (200 µl urine diluted 1:1 with water Wash µl 2 % Formic acid in water Wash µl Methanol Elute 2x 2 µl % Ammonium hydroxide in Acetonitrile/Methanol (60:40 Inject 2 µl Intensity, cps 7.8e4 7.e4 7.0e4 6.e4 6.0e4.e4.0e4 4.e4 4.0e4 3.e4 3.0e4 2.e4 2.0e4 1.e4 1.0e Column: Kinetex 2.6 µm Biphenyl Dimensions: 0 x 2.1 mm Part No.: 00B-4622-AN Mobile Phase: A: 0.1 % Formic acid in Water B: 0.1 % Formic acid in Methanol Gradient: Time (min % B min Flow Rate: 0. ml/min Temperature: Ambient Detection: MS/MS, Triple Quad 400 (SCIEX App ID Absolute Recovery of Amphetamines Amphetamines Concentration (ng/ml (2 % below SAMHSA cut off RT (min % Absolute Recovery % CV (N4 Amphetamine Methamphetamine MDA MDMA MDEA

Method Development / Web Tools / Seminars ur Scientists Work for You ur customers asked us for more SPE support, we responded with PhenoLogix sm,

Take Advantage of PhenoLogix m Method development and optimization m SPE phase screening m Automated high-throughput solutions m n-site method

com/phenologix Create a Customized SPE Method Use our SPE Method Development Tool to create a method in under 1 minute. www.phenomenex.

Schedule a complimentary, customized on-site seminar.

13 Method Development / Web Tools / Seminars ur Scientists Work for You ur customers asked us for more SPE support, we responded with PhenoLogix sm, a full-service analytical support laboratory. Let our PhenoLogix SPE gurus save your lab time and money. Take Advantage of PhenoLogix m Method development and optimization m SPE phase screening m Automated high-throughput solutions m n-site method development m n-site, hands-on training Explore PhenoLogix: Create a Customized SPE Method Use our SPE Method Development Tool to create a method in under 1 minute. Find More Web Tools at: Complimentary Seminars and Webinars Does your lab need to become SPE experts? Schedule a complimentary, customized on-site seminar. Let us know what your challenges are and we can put together a presentation to help you solve them. If you don t have time for us to visit, we can hold online trainings, or view our n-demand Webinars. Seminars@phenomenex.com to Schedule a Webinar Now Watch an n-demand Webinar m Method development m Sorbent selection m Troubleshooting 13

14 rdering Information My Target Analytes Are: H Part No. Your Strata-X Microelution Product and Sorbent Chemistry H S - H Hydrophobic Interaction 8M-S03-4GA H S - H H S - H H Strong Bases (pka >10 H π-π Bonding Weak Cation-Exchange Strata-X-CW 33 µm Polymeric Weak Cation-Exchange Microelution 96-Well Plate, 2 mg/well S - H 8M-S029-4GA S - H H Hydrophobic Interaction H S - H π-π Bonding Weak Bases (pka 8-10 Strong Cation-Exchange Strata-X-C 33 µm Polymeric Strong Cation-Exchange Microelution 96-Well Plate, 2 mg/well H Strata-X 33 µm Polymeric Reversed Phase Microelution 96-Well Plate, 2 mg/well S - H N n.. N 8M-S100-4GA.. N n Hydrophobic Interaction.. N n Neutral -H S Hydrogen Bonding Dipole-Dipole Interactions S - H π-π Bonding N N Weak Acids (pka 2-4 N Microelution 96-Well Plate, 2 mg/well Strata-X-A 33 µm Polymeric Strong Anion-Exchange N Strong Anion-Exchange Hydrophobic Interaction π-π Bonding NH N 8M-S123-4GA NH2 NH N 8M-S038-4GA NH2 NH NH2 NH NH2 Strong Acids (pka <2 NH2 96-Well Plate, 2 mg/well Strata-X-AW 33 µm Polymeric Weak Anion-Exchange NHMicroelution NH2 NH Weak Anion-Exchange Hydrophobic Interaction π-π Bonding N N NH2 NH NH2 NH Learn more about Strata-X Polymeric SPE sorbents and search our application database at If Strata-X Microelution 96-Well SPE Plates do not perform as well or better than your current Microelution 96Well SPE Plate, return the product with comparative data within 4 days for a FULL REFUND. 14 NH NH2 Phenomenex l WEB:

Cost Effective Benchtop Processing Presston 100 Positive Pressure Manifold Part No.

Manifold, 3 ml Tube Complete Assembly AH0-9343 Presston 100 Positive Pressure Manifold, 6 ml Tube Complete Assembly Phenomenex warrants that for a period of 12 months following delivery, the Presston

In the event that the Presston 100 Positive Pressure Manifold does not meet this warranty, Phenomenex will repair or replace defective parts. Please visit www.phenomenex.

15 Cost Effective Benchtop Processing Presston 100 Positive Pressure Manifold Part No. Description AH Presston 100 Positive Pressure Manifold, 96-Well Plate AH Presston 100 Positive Pressure Manifold, 1 ml Tube Complete Assembly AH Presston 100 Positive Pressure Manifold, 3 ml Tube Complete Assembly AH Presston 100 Positive Pressure Manifold, 6 ml Tube Complete Assembly Phenomenex warrants that for a period of 12 months following delivery, the Presston 100 Positive Pressure Manifold you have purchased will perform in accordance with the published specifications and will be free from defects in materials or workmanship. In the event that the Presston 100 Positive Pressure Manifold does not meet this warranty, Phenomenex will repair or replace defective parts. Please visit for complete warranty information. 96-Well Plate Vacuum Manifold Part No. Description Unit AH Well Plate Manifold, Universal w/vacuum gauge ea **Manifold, compatible with 2 ml Impact plate, Novum, b-gone, Phree, Strata, and Strata-X 96-well plate formats. Collection Plates (deep well, polypropylene Part Number Description Unit AH µl/well, Square 0/pk AH ml/well, Square 0/pk AH ml/well, Round/Round Bottom, 7 mm 0/pk AH ml/well, Square 0/pk AH ml/well, Square/Round-Conical Bottom 0/pk AH ml/well, Round/Round Bottom, 8 mm 0/pk Sealing Mats (silicone Part Number Description Unit AH Pierceable, 96-Round Well 8 mm 0/pk Verex Vials Type Finish Material Total Volume Residual Volume Available as Insert Vial µvial i3 (Qsert 11 mm Snap or 9 mm Screw Thread Glass 47 µl < 2 µl Convenience Kits (certified and regular Description 100/pk 1000/pk Vial Kit, Snap, µvial i3 (Qsert, Clear w/ Patch PTFE/Silicone AR AR Vial Kit, Snap, µvial i3 (Qsert, Clear w/ Patch PTFE/Silicone, preslit AR AR Vial Kit, 9 mm, Screw-Top µvial i3 (Qsert Clear w/ Patch PTFE/Silicone AR AR Vial Kit, 9 mm, Screw-Top µvial i3 (Qsert Clear w/ Patch PTFE/ AR AR Silicone, preslit Cert Vial Kit, 9 mm, Screw-Top, µvial i3 (Qsert Clear w/ Patch PTFE/ Silicone, preslit cap* AR C AR C * Bonded-In Septa 1

16 Successful Bioanalytical Sample Preparation Without the Dry Down Microelution 96-Well SPE Plates Increase sensitivity Process small or limited sample volumes Save time and reduce costs Reduce loss of challenging analytes Australia t: 61 ( f: 61 ( Austria t: 43 ( f: 43 ( Belgium t: 32 ( (French t: 32 ( (Dutch f: 31 ( Canada t: 1 ( f: 1 ( info@phenomenex.com China t: f: 86 ( phen@agela.com Denmark t: f: nordicinfo@phenomenex.com Finland t: 38 ( f: nordicinfo@phenomenex.com France t: 33 ( f: 33 ( franceinfo@phenomenex.com Germany t: 49 ( f: 49 ( anfrage@phenomenex.com India t: 91 ( f: 91 ( indiainfo@phenomenex.com Ireland t: 33 ( f: eireinfo@phenomenex.com Luxembourg t: 31 ( f: 31 ( nlinfo@phenomenex.com Mexico t: f: tecnicomx@phenomenex.com The Netherlands t: 31 ( f: 31 ( nlinfo@phenomenex.com New Zealand t: 64 ( f: 64 ( nzinfo@phenomenex.com Norway t: f: nordicinfo@phenomenex.com Puerto Rico t: 1 ( HPLC f: 1 ( info@phenomenex.com Spain t: f: espinfo@phenomenex.com Sweden t: 46 ( f: nordicinfo@phenomenex.com United Kingdom t: 44 ( f: 44 ( ukinfo@phenomenex.com USA t: 1 ( f: 1 ( info@phenomenex.com All other countries Corporate ffice USA t: 1 ( f: 1 ( info@phenomenex.com Italy t: f: italiainfo@phenomenex.com Phenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at international@phenomenex.com Terms and Conditions Subject to Phenomenex Standard Terms and Conditions, which may be viewed at BR302017_W Trademarks Kinetex and Strata are registered trademarks of Phenomenex. Aeris, Impact, Phree, Novum, b-gone, Presston, and Verex are trademarks of Phenomenex. Triple Quad and API 000 are trademarks of AB SCIEX Pte. Ltd. AB SCIEX is being used under license. Strata-X is patented by Phenomenex. U.S. Patent No. 7,119,14 Novum is patent pending Phenomenex, Inc. All rights reserved.

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