PELCO HistoWave Laboratory Tissue Processing System

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Derrick Washington
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Stat Histology Processing with Quality Results PELCO HistoWave Laboratory Tissue Processing System The PELCO HistoWave is a tissue processing system that allows the histology lab to deliver results,

(If your primary interest is decalcification, we recommend our PELCO DFR-10 Tissue Processing System shown on p. 296-298.

1 Stat Histology Processing with Quality Results PELCO HistoWave Laboratory Tissue Processing System The PELCO HistoWave is a tissue processing system that allows the histology lab to deliver results, from fresh tissue through paraffin in 2-4 hours. It can be used for fixation, dehydration, paraffin infiltration, decalcification, immunochemistry and special stains. (If your primary interest is decalcification, we recommend our PELCO DFR-10 Tissue Processing System shown on p The PELCO HistoWave is unique, being the only microwave tissue processor that can efficiently and effectively process tissue for most steps at lower wattages (below 450W), thus lower temperatures, yielding better tissue preservation and quality results. The use of lower wattage/temperature is enabled by the PELCO ColdSpot, which is a temperature-controlled processing surface that removes the uneven hot/cold spots across the bottom surface of the cavity that are characteristic of microwave processors. Examples of protocols using as little as 165W are shown on the next page. HistoWave The base system includes the microwave processor and accessories designed to process samples. Depending on your needs, optional accessories are available such the TissueVac, a vacuum chamber with a tissue cassette holder to make paraffin processing easier and faster; the Sequenza slide holder to facilitate immuno processing; or the PELCO Prep Eze tissue holder to process free-floating sections. This system can be used for light microscope or diagnostic electron microscope approaches. Microwave-assisted processing of human colon endoscopic biopsy after normal 10% NFB fix. 1 x 7 minutes 100% Watts TR of 67 C 1 x 5 minutes ACS reagent Grade Watts, TR of 74 C 1 x 18 minutes Watts, TR of 80 C (400x approximate) H&E. 1

The PELCO HistoWave has many not previously available features for microwave tissue processing such as: The ability to process tissue at low temperatures, allowing for optimum tissue feature and

One power cord. Main Digital Display, which shows sample Probe Temperature or Water Load Temperature or Wattage setting.

2 The PELCO HistoWave has many not previously available features for microwave tissue processing such as: The ability to process tissue at low temperatures, allowing for optimum tissue feature and structure preservation. This feature is only available from us. System level integration with the load cooler, power controller and vacuum/bubbler in one cabinet. No installation or hookup required. One power cord. Main Digital Display, which shows sample Probe Temperature or Water Load Temperature or Wattage setting. True variable wattage power control with 6 preprogrammed settings from 250 to 750 watts. Smart power that automatically compensates for line voltage fluctuation to maintain constant microwave power. Digital display of set power for ease of calibration. LED indication of microwave magnetron "on" or "off". Temperature restriction set point is used to precisely control sample temperature. Digital display of sample temperature. This illustration demonstrates how the PelGraph software will display the progressive, real time specimen temperature on your computer screen when connected to the PELCO HistoWave Processor using the RS 232 outlet. Liver stained with Masson s Trichrome stain using the PELCO HistoWave (20x). Courtesy of Rick Giberson, Ted Pella, Inc. and Bruce Calkins, Pathology Sciences, Chico. 100% ETOH 4 50ml Coplin Jar 315W TR 60 C 6 min. Bouin s Solution 5 Wash in tap water BENCH STEP yellow 6 50ml Coplin Jar 315W TR 40 C 1 min. 20 sec. Gill #2 Hematoxylin 7 50ml Coplin Jar BENCH STEP Wash in tap water to blue 8 50ml Coplin Jar 315W TR 40 C 40 sec. Biebrich Scarlet - Acid Fuchsin 9 Rinse in DI water BENCH STEP 10 50ml Coplin Jar 315W TR 40 C 1 min. Phosphotungstic - Phosphomolybdic Sol 11 50ml Coplin Jar 165W TR 40 C 40 sec. Analine Blue Sol. 12 Rinse in Tap BENCH STEP then DI water 13 50ml Coplin Jar BENCH STEP 30 sec. 1% Acetic Acid Sol. 14 Dehydrate through BENCH STEP ETOH s, clear and mount 2 TED PELLA, INC.

Pelgraph software for temperature charting, alarms, and data filing. Digital display of temperature restriction set point and continuously variable setting knob. PTFE-coated temperature probe.

3 Pelgraph software for temperature charting, alarms, and data filing. Digital display of temperature restriction set point and continuously variable setting knob. PTFE-coated temperature probe. One-touch control pad and timer display. 10 programmable one-touch keypads. Each can be programmed to run four stages up to 60 minutes, or all four included together for a total of up to four hours. Manual time entry in minutes and seconds up to 60 minutes. Digital timer display counts down processing time. Built in load cooler recirculator to maintain an ambient uniform processing environment. Digital display of load temperature. Inclusion of the patented PELCO ColdSpot, a temperature controlled processing surface which eliminates hotspots. This accessory allows you to use microwave exposure to process your sample without relying on specimen heating alone. Built in air bubbler for adjustable sample solution agitation. Integrated vacuum with continuous or automatic operation for variable time and pump/vent cycling. Vacuum Gauge displays vacuum achieved. Vacuum and bubbler ports conveniently located near the front of the Microwave cavity. Dual fans that provide continuous exhaust, supplying negative air movement when the chamber door is opened, keeping reagent vapors moving up the exhaust. Full line of accessories: PELCO TissueVac Vacuum Chamber; Sequenza Slide Processor, PELCO PrepEze Tissue Holder. Laboratory engineered: The only UL - Certified microwave tissue processing system intended for laboratory use. References Rassner UA, Crumrine DA, Nau P, Elias PM, Microwave incubation improves lipolytic enzyme preservation for ultrastructural cytochemistry. Histochem J 29: Schray CL, Metz AL, Gough AW, Microwave-Enhanced Fixation for Rapid Preparation of Tissue Sections for Microscopic Evaluation. Histologic 35:1, pp Intestine, cut at 4 microns, stained with Mucin Alcian Blue ph2.5 using the PELCO HistoWave (20x). Courtesy of Rick Giberson, Ted Pella, Inc. and Bruce Calkins, Pathology Sciences, Chico % ETOH 4 50ml Coplin Jar 165W TR 45 C 30 sec. 3% Acetic Acid 5 50ml Coplin Jar 165W TR 45 C 1 min. 30 sec. Alcian Blue Sol. 6 Wash in DI water BENCH STEP 7 50ml Coplin Jar 165W TR 45 C 1 min. 30 sec. Nuclear Fast Red 8 Rinse in DI water BENCH STEP 9 Dehydrate through BENCH STEP ETOH s, clear and mount 3

The PELCO ColdSpot in place in the PELCO HistoWave Chamber. Also shown, the PELCO TissueVac. Specifications: PELCO HistoWave Microwave frequency Cabinet exterior 2.45 GHz 55.25 W x 55.9 H x 48.

4 The PELCO ColdSpot in place in the PELCO HistoWave Chamber. Also shown, the PELCO TissueVac. Specifications: PELCO HistoWave Microwave frequency Cabinet exterior 2.45 GHz W x 55.9 H x 48.25cm D (21-3 4" x 22" x ") Cabinet door open 90 degrees 87.6cm Depth (34-1 2") Oven Interior 34.3W x 24.1H x 39.8cm D (13-1 2" x 9-1 2" x ") Magnetron power watts (adjustable, 6 settings), 120V or 230V Electrical power required Weight Certifications 120V, 20Amp, 60Hz 230V, 16Amp, 50Hz 47.7 kg (105 lbs) 120V UL and C/UL for Laboratory Use USA and Canada CE, 230V Europe Community and others Registration with the US FDA as a medical device Ordering Information: PELCO HistoWave Laboratory Tissue Processing System PELCO HistoWave Laboratory Tissue Processor, Base System, 110VAC, 60Hz, complete. Includes: Bubbler Hose; Vent with 2.44 meters (8 feet) duct hose and elbow; water load connections; temperature probe and stand; digital temperature readout and settings; Pelgraph software; RS 232 outlet; PELCO Coldspot ; operation manual PELCO HistoWave Laboratory Microwave System, 230VAC, 50/60Hz, complete. Includes: (details as above for 37500). Made in the U.S.A. 4 TED PELLA, INC.

PELCO 36160 TissueVac Histology Vacuum Accessory Intestinal cross-section, cut at 4 microns, stained with PAS using the PELCO HistoWave (20x). Courtesy of Rick Giberson, Ted Pella, Inc.

Schiff Reagent 7 50ml Coplin Jar 165W TR 45 C 4 min. Warm Tap Water 8 Wash in tap water BENCH STEP 9 50ml Coplin Jar 165W TR 45 C 40 sec.

ColdSpot are registered trademarks and Pelgraph, PELCO Prep-Eze and PELCO TissueVac are trademarks of Ted Pella, Inc. All rights reserved. Ted Pella, Inc. 02-04.

5 PELCO TissueVac Histology Vacuum Accessory Intestinal cross-section, cut at 4 microns, stained with PAS using the PELCO HistoWave (20x). Courtesy of Rick Giberson, Ted Pella, Inc. and Bruce Calkins, Pathology Sciences, Chico % ETOH 4 50ml Coplin Jar 315W TR 60 C 2 min. 30 sec. 0.5% Periodic Acid 5 Wash in DI water BENCH STEP 6 50ml Coplin Jar 165W TR 45 C 2 min. Schiff Reagent 7 50ml Coplin Jar 165W TR 45 C 4 min. Warm Tap Water 8 Wash in tap water BENCH STEP 9 50ml Coplin Jar 165W TR 45 C 40 sec. Gill #1 Hematoxylin 10 Wash in DI water BENCH STEP 11 Blue in Scott s water BENCH STEP 12 Wash in tap water BENCH STEP 13 Dehydrate through ETOH s clear and mount PELCO, PELCO HistoWave and PELCO ColdSpot are registered trademarks and Pelgraph, PELCO Prep-Eze and PELCO TissueVac are trademarks of Ted Pella, Inc. All rights reserved. Ted Pella, Inc PELCO TissueVac accessory shown with Cassette Holder in Vacuum Chamber/ Processing Container with Temperature Probe inserted The PELCO TissueVac Histology Vacuum Accessory is designed for the PELCO BioWave and can be used in conjunction with the PELCO ColdSpot. It can be used with any of the three vacuum settings on the BioWave (vacuum cycle, vacuum on W/MW, vacuum on) or without the vacuum function. The container and lid are made from microwave transparent polypropylene. The integrated temperature probe allows the use of temperature Cassette Holder with cassettes restriction conrol with vacuum. The cassette holder will hold up to 58 standard size plastic cassettes, all in the same orientation and plane. This assures that every tissue sample will see the same microwave conditions. The granite stone is used as a heat receptor during paraffin infiltration. The PELCO TissueVac accessory is comprised of the following components: Vacuum Chamber / Processing Container Lid Assembly PTFE-coated Temperature Probe L-Gasket Nylon Strap Cassette Holder (PTFE) for 58 cassettes Granite Stone Operation Manual PELCO TissueVac System, each Processing Container, each Cassette Holder, each 5

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