Thermo-Finnigan LCQ Interface Manual
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1 Thermo-Finnigan LCQ Interface Manual For Thermo Finnigan LCQ XP, Deca, Advantage, Duo, and Classic Models Version IonSense Inc. 999 Broadway Suite 404 Saugus, MA 01906
2 Copyright by IonSense Inc. All rights reserved. The information in this document has been carefully checked and is believed to be reliable. However, no responsibility is assumed for inaccuracies. Statements in the document not intended to create any warranty, expressed or implied. Specification and performance characteristics of the hardware and software described in the manual may be changed at any time without notice. IonSense Inc. reserves the right to make changes in any product herein in order to improve reliability, design, or function. IonSense does not assume any liability arising out of application or use of any product or circuit described nor does it cover any license under its patent rights or the rights of others. The apparatus and application of the apparatus described in this document is protected by US Patent Number 6,949,741 and used under license; additional patents pending. All trademarks are properties of their respective owners. 2
3 This manual details the steps necessary to install a DART source on a Thermo-Finnigan LCQ mass spectrometer. Table of Contents Installing the DART on The LCQ Deca and Classic Models... 4 Installing the DART on LCQ XP, Duo, and Advantage Models... 7 Recommended Optimal Settings
4 Installing the DART on The LCQ Deca and Classic Models First, place the mass spectrometer in standby mode and remove the Thermo-Finnigan source from the LCQ mass spectrometer. Clean the source region and the end of the heated capillary. Figure1: The LCQ Deca and Classic DART flange with nuts and interlock pin Next, place the interlock pin in the small hole in the bottom-right corner on the back of the flange. Figure 2: The interlock pin inserted into the hole on the back of the flange 4
5 Attach the flange by holding the flat side on the bottom. Take care that the interlock pin does not fall out while pressing it into the flange. If you are using a DART SVP source, screw the two interlock pins into the holes highlighted in the figure below. (Your flange may not have the same number of holes as the flange shown in the picture below.) On the bottom of the source, remove the waste line if you have not done so already. Take the black silicone rubber tubing and attach the open end to the waste port. The other end should be attached to a vacuum source, either the Vacuubrand pump ordered from IonSense, or another pumping source provided by the user. 5
6 The silicone rubber tubing connected to the waste port. Insert the ceramic tube into the Swagelok nut on the end of the VAPUR attachment and secure it with the graphite ferrule. Take care that there is a 2mm gap inside the VAPUR between the end of the ceramic tube and the protruding end of the Agilent capillary. This ensures that the excess helium gas will be evacuated and maintain proper vacuum in the instrument. Finally, attach the DART by either securing the two captured screws in the front ring to the flange, or removing the ring and bolting the two holes on the front of the DART base to the bottom of the flange. 6
7 Installing the DART on LCQ XP, Duo, and Advantage Models To install the DART on the LCQ XP, Duo, or Advantage, follow the same procedure as above with the larger extended-barrel SI-110 flange. One notable difference between the SI-100 (LCQ Deca) and the SI-110 (LCQ XP) flanges are that the LCQ XP flange has the interlock pin fastened to the flange. It does not need to be inserted or removed. s 7
8 Recommended Optimal Settings These settings are intended as a rough guideline under which to operate your LCQ-DART. IonSense recommends tuning the mass spectrometer yourself with your desired analyte. These settings may not be optimal on every model of LCQ and some settings may not exist or be variable on other models. For LCQ Deca Models: ESI Source (Positive Mode) Sheath Gas Flow Rate (arb): 0 Aux Gas Flow Rate (arb): 0 Spray Voltage (kv): 0 Capillary Temp (C): Capillary Voltage (V): Tube Lens Offset (V): 0.00 Ion Optics (Positive Mode) Multipole 1 Offset (V): Lens Voltage (V): Multipole 2 Offset (V): Multipole RF Ampliture (V p-p): Entrance Lens (V): ESI Source (Negative Mode) Sheath Gas Flow Rate (arb): 0 Aux Gas Flow Rate (arb): 0 Spray Voltage (kv): 0 Capillary Temp (C): Capillary Voltage (V): Tube Lens Offset (V): 0.00 Ion Optics (Negative Mode) Multipole 1 Offset (V): Lens Voltage (V): Multipole 2 Offset (V): 7.00 Multipole RF Ampliture (V p-p): Entrance Lens (V):
Thermo-Finnigan LCQ Interface Manual
Thermo-Finnigan LCQ Interface Manual For Thermo Finnigan LCQ XP, Deca, Advantage, Duo, and Classic Models Rev 2 Document # 7.5.062 IonSense Inc. 999 Broadway Suite 404 Saugus, MA 01906 Copyright 2005-2010
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