Efficacy of Prestop against soil borne and foliar pathogens on European crops
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1 Efficacy of Prestop against soil borne and foliar pathogens on European crops
2 Core business : Production of micro-organisms Bakker s yeast and products Biers Animal Care (health/nutrition) Wine Human Care (health/nutrition) Santé & nutrition humaine Distilled alcools and ethanol Plant Care (health/nutrition) Bio-ingredients
3 Plant Care Unit : Ithec and Verdera Biological plant protection products and biofertilizer for agriculture, horticulture and forestry
4 Biological plant protection products for horticulture Prestop based on Gliocladium catenulatum strain 1446
5 Current Registrations of Prestop PRESTOP ONGOING REGISTRATION US and European approval (EPA and Annex 1)
6 Ecological characteristics of strain J1446 Isolated from Finnish field soil within a Nordic project on biocontrol of seed-borne pathogens of cereals Biological activities : between 6 and 30 C Able to survive below 6 C and above 30 C Not harmfull to beneficial insects, nematodes or pollinators Gliocladium catenulatum J1446
7 Ecological characteristics of strain J1446 Gliocladium catenulatum J1446 is able to colonize leaf and root surface G. catenulatum growing on water agar, isolated from cucumber roots. G. catenulatum growing on potato dextrose agar, isolated from pelargonium leaves.
8 Modes of action of G. catenulatum J1446 Various modes of action are involved Hyperparasitism seems to play an important role : Detection of enzyme activities Observation of mycelium interaction Competition for nutrients and space Colonization of root and foliar surfaces Antibiosis not shown Weak probability of development of pathogen resistance
9 The compatibility between Gliocladium catenulatum and chemical pesticides Teldor (fenhexamid) In vitro Switch 62.5 WG (cyprodinil and fludioxonil)
10 The compatibility between Gliocladium catenulatum and chemical pesticides In vivo Full compatibility with Topsin M (tiophanate-methyl) on cucumber when sprayed at the same day
11 Active ingredient Examples of commercial name Interval (days) Compatibility between Gliocladium catenulatum and fungicides Azoxystrobin Amistar 2 Benomyl Benlate 4 Boscalid et krésoxim-méthyl Collis 0 Bitertanol Baycor 2 Carboxin Cadan, Oxalin, Vitavax 4 Fenhexamide Teldor 0 Fludioxonil-cyprodinil Switch 4 Guazatine Panoctine 2 Hymexazol 7 Imazalil Fungaflor 2 Iprodione Chipco Green 75WG / Rovral 4 Krézoxym-méthyl Stroby, Candit 0 Mancozep Dithane, Mancozeb 4 Mépanipyrim Frupica 0 Métalaxyl-M 0 Myclobutanil Systhane 24 EC 0 Penconazole Topenco, Topas 100 EC 1 Phosétyl-aluminium Aliette 0 Prochloraz Sportak 45 HF 7 Procymidone Fortress Propamocarp Hydrochloride Previcur Energy, Previcur N 0 Propiconazole + prochloraz Basso 7 Pyraclostrobin+boscalid Signum 2 Pyriméthanil Scala 1 Sulfur 0* Thiophanate méthyl Topsin M 2 Toclofosmethyl Rizolex 2 Thiram Thirame, TMTC, TMTD 4 Triadiméfon Amiral, Baylaton 0 Triadiménol Baytan 2 Triforine Funginex 2 Trifloxystrobin +propiconazole Stratego 4 Triflumizole 0 Vinclozolin Ronilan 4
12 Compatibility between Gliocladium catenulatum and insecticides Active ingredients Days Bacillus thuringiensis 0 Beauveria bassiana 0 Buprofezin 0 Cypermethrin 2 Deltamethrin 0 Diazinon 0 Fenbutatin oxide 2 Malathion 0 Metharizium anisopliae 0 Mevinphos 0 Permethrin 2 Pirimicarb 0 Pyrethrins 0
13 USES in CONVENTIONAL, IPM and ORGANIC CROPS 1. Treatments of substrates against Pythium, Phytophtora, Rhizoctonia, Fusarium 2. Treatments of vegetable, ornamental and aromatic plants, against Pythium, Phytophthora, Rhizoctonia et Fusarium 3. Treatments against black rot of cucumbers (Didymella) 4. Treatments against grey mould caused by Botrytis on tomatoes, green pepper, cucumbers, strawberries, and ornamental plants
14 Biological activity of Gliocladium catenulatum strain J1446 with Prestop formulation Foliar diseases
15 Prestop on tomatoes against Botrytis cinerea In the Netherlands and Belgium, annual crop losses in spring and autumn (up to 25%) Airborne spores always available Growth conditions: high moisture (> 87%) weak plant tissues (pruning wounds)
16 Trial in 2008 : Prestop on tomatoes against Botrytis (spraying on stems) Protocol : Tomato crop 25 weeks old The rate of Prestop was 100 g suspension per 1000 plants (20ml per stem) per application Three times replicated (wk 42, 44, 46) Pruning wounds preventively treated by spraying the stems and then inoculated with Botrytis spore suspension Treated wound covered during 48 hours Lesion development on wound UR, Wageningen, Nederland, Autumn 2008
17 Trial in 2008 : Prestop on tomatoes against Botrytis (spraying on stems) Results b b a a Preventive application of Prestop = Total protection like chemical treatement UR, Wageningen, Nederland, Autumn 2008
18 Trial in 2011 : Prestop on tomatoes against Botrytis (spraying on wounds) Results b b b a a IWT, Belgium, 2011
19 Prestop in the control of gummy stem blight of cucumber (Didymella/Mycosphaerella) Spray of the stems 6 and 53 days after planting 25 23,4 Infected plants % ,6 Untreated Prestop 100g/1000 plants
20 Trials in 2011 and 2012 : Low volume spraying of Prestop in foliar treatment of tomatoes LV-spraying (fine fog) was used in a commercial tomato cultivation in Holland The pressure was 6 bars and the solution was pumped through a filter-nozzle combination An excellent colonization of Gliocladium in the foliage was observed after LV-spraying: Gliocladium index (0-3) in larger leaves: average 2.8 Gliocladium index (0-3) in smaller leaves: average 2.8 No observation of Botrytis (in vitro and in the greenhouse) Tomato producers, Nederland, 2011 and 2012
21 Novel applications on grapevine Trials in 2010 and 2011 in Austria by Kwisda 2010 : test at 5 kg 2011 : test at 1 kg and 2 kg with or without copper
22 Control of grapevine grey mould : 1st site Kwisda experiment
23 Control of grapevine grey mould : 2nd site Kwisda experiment : Similar results with Zweigelt variety in Lower Austria
24 Biological activity of Gliocladium catenulatum strain J1446 with Prestop formulation Root diseases Pythium, Phytophtora, Rhizoctonia, Fusarium
25 Prestop in the control of root diseases on sweet pepper, applied via drip irrigation Trial 15/01
26 Prestop in the control of damping off (Pythium and Rhizoctonia), 6 weeks after sowing Control Prestop by drenching 10g/m 2
27 Prestop spraying on Pelargonium infected by Phytophtora Peat Potting soil Phytophthora Phytophthora + Prestop WP Phytophthora Phytophthora + Prestop WP
28 Methods of applications Application methods for root and foliar diseases : Spraying of the growing medium Incorporation in the liquid solution for hydroponic cultures and drip irrigation Spraying with standard equipement at normal and low volume (LVspraying system) Spray the pruning wounds with a small hand-sprayer against Botrytis on tomatoes
29 Conclusions: Gliocladium catenulatum strain J1446 as a biocontrol agent Strain J1446 controls several root diseases as well as foliar pathogens on vegetable, fruit, ornemental and aromatic plants Strain J1446 is compatible with many other plant care products Prestop is registrated in American and European countries Prestop is widely used in greenhouse and more and more in field Formulation is adapted to standard and alternative systems of application
30 Thank you for your attention
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