10/26/2016. IR-4 Meeting Orlando, FL 09/16

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Beryl McDonald
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1 IR-4 Meeting Orlando, FL 09/16

2 ASPERELLO Dry spores of Trichoderma asperellum T34 Isolated from Fusarium suppressive compost Highly concentrated (10 9 cfu/g) active at low dose rate Multiple modes of action resistance management

3 Multiple modes of action How does ASPERELLO work? Competition Induced resistance Parasitism No risk for resistance development Efficient in many situations Against numerous diseases

4 ASPERELLO Current EPA registration in US (EPA Reg. No: ) for Ornamentals in Greenhouses Fusarium Pythium Phytophthora Rhizoctonia

5 ASPERELLO Label Expansion to: Outdoor Ornamentals/ Tree Nurseries Edible Crops New Niches (Black Sigatoka in Bananas?)

6 Carnation Gladiolus Cyclamen Poinsettia Pansies Hedera Chrysanth emum Turf Tomato Pepper Lettuce Cucumber Melon Watermelo n Strawberry Potato Onion Green bean Sunflower Corn Vineyard Trials show potential in Fusarium oxysporum X X X X X X X X Fusarium lactis X Rhizoctonia solani X X X X X X X Sclerotinia sclerotiorum X X Pythium spp. X X X X X X Phytopthora capsici X Macrophomina phaseolina X Sclerotium cepivorum X Phytophthora cactorum X X Erwinia spp. Leptosphaeria lindquistii Ralstonia solanacearum Cephalosporium maydis Sphacelotheca reiliana X X X Botrytis cinerea X X X X Didymella bryoniae X X X X

7 Disease severity Disease score ASPERELLO Fusarium Pythium a c ab b a b b

Health rating 50 days post planting ASPERELLO Fusarium oxysporum -Chrysanthemum 6 5 a ab Asperello 4 b bc Inoculated 3 cd 2 d 1 0 non-treated Inoculated,

8 Health rating 50 days post planting ASPERELLO Fusarium oxysporum -Chrysanthemum 6 5 a ab Asperello 4 b bc Inoculated 3 cd 2 d 1 0 non-treated Inoculated, Non-treated ASPERELLO (4 treatments) ASPERELLO Heritage 50 WP (drench) (preincorp. only) Trichoderma reference (preincorp. only) M. Daughtery, Cornell (2016)

9 ASPERELLO Botrytis cinerea - Strawberries 12 % acumulado de frutos afectados totales respecto a cosechados b b a b 0 Botrytis y Folpet Botrytis y Switch Botrytis Botrytis y T34

ASPERELLO Greens/Lettuce Sclerotinia sclerotiorum Root colonization Stimulation of plant growth Also in absence of pathogen 0,35 Area Under Disease

10 ASPERELLO Greens/Lettuce Sclerotinia sclerotiorum Root colonization Stimulation of plant growth Also in absence of pathogen 0,35 Area Under Disease Progress Curve 0,30 0,25 0,20 0,15 0,10 0,05 ab c a bc 0,00 Sclerotinia + Rizolex Sclerotinia + Switch Sclerotinia Sclerotinia + T34 Asperello Asperello

11 ASPERELLO Pythium aphanidermatum EU registration (Pythium Solanaceaous): BE, NL, FR, UK

12 ASPERELLO Black Sigatoka Mycosphaerella in Banana

13 ASPERELLO Trials in Melon + Watermelon: control of Mycosphaerella

14 IR-4 Worskhop Orlando, FL, September 2016 Using Biobest Flying Doctors (FD) for targeted application of biopesticides Sarah van Beneden Presented by: Veronica Cervantes

15 Pollination + Crop Protection Entomovector technology (Bee vectoring) + = vector Biological control agent FD

16 Biobest Flying Doctors System Patented one-way traffic design......ensures product pickup only when bumblebees leave the hive

6kg/ha Greenhouse trial / / / / Frupica 0.6kg/ha 8 g per hive /3days Teldor 1.

17 Flying Doctors System Before flowering Flowering Ripening Untreated / / / / / Teldor (1x) Prestop Mix Chemical control Teldor 1.5kg/ha Frupica 0.6kg/ha Greenhouse trial / / / / Frupica 0.6kg/ha 8 g per hive /3days Teldor 1.5kg/ha Teldor 1.5kg/ha Signum 1kg/ha % Strawberries with B. cinerea 14 days post harvest (storage at 4 C,high RH) Prestop 4B

18 Flying Doctors System Open field + Conventional spraying! Botrytis infection in the field Trial 2 Trial 1 FD FD

19 % Healthy fruits Prestop 4B + Flying doctors Biological control of Botrytis in Raspberries 4D post harvest 7D post harvest Conventional 10D post harvest

20 Blueberries Mummy Berry FD + Bacillus subtilis WP Biological solution for Monilinia vaccini-corymbosi Serenade in Organic blueberry production Gives Fair to Good control Protectant Source: Michigan Blueberry Facts: Mummy Berry (E2846)

21 Biobest Flying Doctors System Thank you for your attention! Questions?

Biological Fungicide. Trichoderma asperellum strain T34. Prevents disease and protects. crops naturally TECHNICAL DOSSIER

Biological Fungicide. Trichoderma asperellum strain T34. Prevents disease and protects. crops naturally TECHNICAL DOSSIER Biological Fungicide Trichoderma asperellum strain T34 Prevents disease and protects crops naturally TECHNICAL DOSSIER T34 Biocontrol biological fungicide Prevents disease and protects crops naturally