Automated Sample Preparation: SPE GPC - EVAporation SUPERIORITY BY ADAPTATION. Visit us at
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1 Automated Sample Preparation: SPE GPC - EVAporation SUPERIORITY BY ADAPTATION Visit us at
2 What LCTech does supplies innovative products and methods for the preparation and analysis of food, feed and environmental samples.
3 Mission Our misson: THE CLEANER THE EXTRACT THE BETTER THE RESULT
4 FREESTYLE System - Introduction This system is designed for sample preparation mainly in food, feed and environmental laboratories, but can be used in any lab in which GPC and/or any type of SPE columns are used for clean-up
5 FREESTYLE The Concept FREESTYLE is a system which is based on FREESTYLE BASIC and can be modified with modules SPE module FREESTYLE BASIC GPC module EVAporation in all imaginable combinations
6 FREESTYLE BASIC FREESTYLE BASIC Valves, syringe pump & others on top of y-axis (reduces dead volume in tubing) xyz-robotic system Rinsing port for needle Flexbile robotic area; the appropriate area can be created at any time by choosing the right racks
7 FREESTYLE Modules SPE Module SPE module as a 2nd z-axis Universal manipulator for all standard SPE-/ Immuno affinity - (IAC) and Glass columns (e.g. LCTech columns) from 1 to 15 ml Control unit for SPE module
8 FREESTYLE Modules FREESTYLE BASIC SPE Module
9 FREESTYLE Modules GPC module GPC-column: different sizes for all known official methods available accessory; not included in the module Injection valve High precision, double piston GPC-pump Exchangeable sample loop
10 FREESTYLE Modules FREESTYLE BASIC GPC module
11 FREESTYLE Modules EVAporation module Integrated vacuum pump, heater & electronical devices Solvent recovery/ solvent recycling Water for temperature control of the vacuum chamber Heart of the EVAporation system: EVAporation chamber with sensors
12 FREESTYLE Modules FREESTYLE BASIC EVAporation Module
13 FREESTYLE adapted to applications Typical combination of different modules: FREESTYLE BASIC + GPC + EVAporation: For sample preparation (clean up) for pesticides / PAH and all other analytes in fatty matrices which have to be cleaned up before transfering to analytical instruments (eg. GC or LC). Sample preparation for soil analysis For a faster, reliable, more reproducible and more comfortable handling of such samples customer can choose a higher level of automation: FREESTYLE BASIC with GPC module for clean-up and EVAporation module
14 FREESTYLE Modules FREESTYLE BASIC GPC module EVAporation Module
15 FREESTYLE modified for applications Typical combination of different modules: FREESTYLE BASIC + SPE + EVAporation: Automation of SPE Handling in all kind of labs For a faster, reliable, more reproducible and more comfortable handling of such samples customer can choose a higher level of automation: FREESTYLE BASIC with SPE module and EVAporation module
16 FREESTYLE modified for applications FREESTYLE BASIC SPE-Modul EVAporation Module
17 FREESTYLE fully automated SPE-Modul FREESTYLE BASIC GPC-Modul EVAporation Module
18 SUPERIORITY BY ADAPTION Your advantage SUPERIORITY BY ADAPTATION
19 The unique design Sorry, FREESTYLE does not offer 50 or more platforms! FREESTYLE has just one, but you can create this one according your needs, your demands, at any time (no service or tools needed) Racks can be individually positioned by hooking them into a toothed plate
20 Racks the key for flexibility This pictures shows how it works
21 Racks the key for flexibility frame = rack tray with vials Frame canbeusedfor different kinds of vials Tray can be taken out of frame and prepared independently
22 Racks the key for flexibility for vials from 1.0 ml to 1000 ml (e.g. standard flasks, round bottom flasks, TurboVap, Büchi, test-tubes ) racks can be cooled or heated With protection for light sensitive samples Vials and flasks can be sealed with septa = You can choose between r.a. 30 different racks. And if you cannot find the appropriate rack we are sure that we can design it. Ask LCTech!
23 Powerful Software Selection of individual modules or combination of different functions within a single method (eg. SPE +EVA or GPC+EVA) no hardware changes necessary! Allows intuitive handling and easy usage in daily routine Indicates most of the logical failures done by the customer choosing the wrong parameters Always included in the purchase price of modules
24 Powerful Software Main Window
25 FREESTYLE system features Integral system features Extensive safety devices: - Possibility of immediate emergency stop in case of danger or to prevent any dangerous situation - Collisions recognition with the possibility to proceed without loss of sample All liquid containing tubing is located outside the instrument, thus easily accessible and strictly separated from any electric wiring Plausibility check of software input Software is always included in the modules
26 FREESTYLE - Standards of production Standards of development and production & safety guidelines Developed and produced at highest level according to Machine Directive 2006/42/EC of the European Parliament and of the Council of 17 May 2006 on machinery, and amending Directive 95/16/EC (recast), including CE certification. Developed and produced according to ISO 9001:2008. CE labelled safety guidelines
27 SPE Module For all standard SPE-cartridges/columns from 1 ml up to 15 ml For all standard Immunoaffinity columns (1 ml/3 ml) IAC-columns can be stored in the rack sealed with a tight cap, so buffer does not flow out. IAC remains fresh until you decide to use it For glass columns for max. dimensions 105 x 17,5 ID (length x diameter)
28 SPE Module Positive pressure for all steps in which liquids are used One rack for all kind of columns: reusable adapters allow an easy to change and mixed setup for all kinds of columns Columns can be moved to any place in the robotic area Standard three solvents, upgrade option up to six solvents
29 SPE Module SPE Module & handling of SPE cartridges/spe columns Software allows the transfer of nearly any manual method straight to an automation Method Development Move next step from the basic tools with drag & drop to second column The default button remains in the basic tools and can be choosen again The collected steps in the second column build the method Each button there can be individually changed and adopted by using the bars.
30 Software: SPE method SPE Module
31 SPE Module Application:determination of the Hydrocarbon Oil Index in water/soil according to EN ISO (H53 Method) For this very common routine application a special rack is used 36 samples can be handled in sequence If desired FREESTYLE system (Evaporation module necessary) can evaporate them right away down to 1.0 ml, sample can be transferred in GC-Vial: ready for analysis
32 EVAporation Module All necessary items are integrated (only chiller for solvent recovery additionally needed) Heart of the system: EVAporation chamber frequency controlle diaphragm pump (Vacuubrand) all sensoric items for detection of final volume heating system with two zones a) with water for fast EVAporation b) electrical heater for the extended cone for very sensitive evaporation EVAporation at its best!
33 EVAporation Module You can use these unique and unbeatable functions: automated EVAporation down to a final volume final volume can be choosen between 0.5 ml and 5.0 ml final volume can be set by software; no manual adjustment of the sensors! final volume can be set individually for each method = that means each sample in a sample list can have a different final volume EVAporation either by vacuum/heat or by nitrogen/heat (option) for very sensitive samples EVAporation at its best!
34 EVAporation Module More of these unique and unbeatable functions: solvent exchange (fluid/fluid or dryness/resolved) 360 C rinsing of the EVAporation chamber Concentrate can be fractionated Transfer of the concentrated sample with the robotic needle and not aspirated through a tube Option: the whole system can be charged with nitrogen Last but not least: automated calibration of the EVAporation chamber at any time for reliable and high precision final volume EVAporation at its best!
35 EVAporation module EVAporation method development; what you can use and modify: Sample aspiration Vacuum process Blow-down with nitrogen or compressed air Sample withdrawal from vacuum chamber Solvent exchange Fill-up and aliquotation Vacuum chamber rinsing EVAporation at its best!
36 Software: EVAporation method EVAporation Module EVAporation at its best!
37 GPC Module Double piston pump, esp. modified for low pressure applications (esp. with the appropriate pressure sensor) Flow rate from 0.1 ml to 50 ml/min GPC columns: - different dimensions and - different solvents for all official sample clean up methods worldwide
38 GPC Module GPC method development (1): 5 different possibilites of injection for all known applications: - from sample loop overfill (traditional clean up for pesticide analysis) - up to quantitative transfer by several injections from one glass (incl. 360 spray rinsing) - and collection of these runs in one flask (dioxin-analysis) Definition of forerun, main run (total, partial or sequential fractionation) and tailings Number and size of sample and collect vials Depth of needle immersion
39 GPC Module GPC method development (2): Column injection counter Flow rate (GPC module) Min. and max. pressure shut-off Sample aspiration speed (important for solvents like DCM) Column type and specification, respectively UV Detector data acquisition (yes/no)
40 GPC Module Software: GPC method screen; the whole adjustment of a GPC method can be done in this easy to handle screen.
41 FREESTYL - General Accessories Barcode Reader - Reading width 65 mm - With USB connection cable
42 FREESTYLE - General Accessories Liquid level switch for organic solvent (e.g. for waste bottle) Check of liquid level of waste bottle: Interruption of process, if bottle is empty / full (displayed in software)
43 FREESTYLE - Accessories for SPE and EVA Upgrade: 3 to 6 solvents: - Highly recommended for SPE applications Blow-down function for nitrogen and compressed air: - necessary for usage of nitrogen/air for evaporation - and drying of columns in SPE-function;
44 FREESTYLE Accessories for GPC module UV Fixed Wave Length Detector (254nm) for GPC column performance test; necessary software is integrated in the software package for FREESTYLE-systems
45 FREESTYLE - SUPERIORITY BY ADAPTATION Flexibility on highest level: FREESTYLE BASIC + GPC-Module + EVAporations-Module + SPE-Module In one sample list, there can be combined any method totally independent from each other: e.g.: 1. run: GPC 2. run: GPC+ EVA 3. run: GPC + EVA + SPE 4. run: EVA There is no hardware modification necessary at any time!
46 Where to find us Dorfen, Home of Bahnweg Dorfen Germany More about FREESTYLE-systems on or visit us at
Systems. Visit us at
Systems And especially: SPE Module Visit us at www.lctech.de! Systems General e Description Highlights Modules Structure and Availability FREESTYLE TM BASIC FREESTYLE TM SPE 2 FREESTYLE General Description
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