Supra-Clean and Supra-Poly SPE Solutions. Solid Phase Extraction Columns
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1 Supra-Clean and Supra-Poly SPE Solutions Solid Phase Extraction Columns
2 Imagine how much faster, easier and more efficient your sample preparation would be if your Solid Phase Extraction (SPE) solutions were precisely manufactured for guaranteed reproducibility. Not just column to column. Not just batch to batch. But lot to lot no matter how many days, weeks or years between production times. That s exactly the kind of confidence and convenience you can expect with the innovative line of Supra-Clean silica-based and Supra-Poly polymer-based SPE cartridges and columns from PerkinElmer. Our Precise-Bed Technology provides precision and manufacturing tolerances of + 1%, ensuring guaranteed repeatability and optimized recovery reproducibility. The distribution and size of particles is controlled through stringent manufacturing processes, and each finished product is delivered with an individual quality certificate. Experience the difference for yourself. Or to be more exact experience the uniformity. SPE Column Competitor SPE Column Spherical silica and consistent particle distribution enable smaller elution volumes and better, more reproducible purification, concentration and recovery. Performance Comparison of PerkinElmer Spherical SPE Media vs. Leading Irregular SPE Silica x 4.6 mm column Hexane - IsoPropanol (97/3) Flow rate: 1.0 ml/min PSI: 1 bars UV: 254 nm A- Tri-Terbutylbenzene B- Diethyl Phthalate IR60-40/63SI SPE Supra-Clean 60-50SI V 1 < V PerkinElmer s smaller, more homogeneous, spherical media deliver sharper, narrower peaks for faster, more accurate sample insight. V 1 V 2 Consistently. Excellent. Performance. The unsurpassed reproducibility of SPE solutions is the result of a unique bed-packing technique and spherical media. Columns are evenly and consistently filled with particles sized for optimum distribution, delivering a level of performance unattainable with the irregularly shaped silica used in competitive solutions. PerkinElmer s homogeneous filling and mono-dispersed spherical media not only provide unparalleled reproducibility, they also deliver superior performance. Sample diffusion through the media is better than in other systems, improving flow efficiency and avoiding channeling and clogging due to fines. This enables smaller sample sizes to be used without compromising recovery levels, dramatically reducing solvent volumes, analytical costs and processing times. With PerkinElmer SPE solutions, you can work faster and more costeffectively, all while experiencing the complete recovery, reproducibility and reliability you need for consistently excellent results. Answering Your Needs. Empowering Your Lab. Ideal for a broad array of analytes and matrices, SPE solutions are available in a variety of formats including Large Reservoir Capacity (LRC) columns, Polypropylene (PP) columns and cartridges, and glass columns. Each technology is offered with a wide selection of polymer and silica sorbents, and large and small sample volumes (50µL-1L) allow you to perform scalable analyses depending upon your required detection limits. 2
3 Choosing a Solution to Suit Your Method To find the ideal SPE column for your particular application, simply follow the flow diagram below, selecting your path based on the options at each junction. Your Sample Type of Matrix Aqueous Organic Analyte Solubility Water Follow same path as Organic Water Organic Solvents Analyte Neutral Ionic (Follow same path as Ionic analyte) Ionic Neutral Neutral Analyte Polarity Non-Polar Moderately Polar Polar Cationic Bases Anionic Acids Non-Polar Non-Polar Polar Recommended Reverse Phase Ion Exchange Reverse Phase Normal Phase Recommended SPE Products Supra-Clean C18 Supra-Clean 300Å C4 Supra-Clean Phenyl (PH-S) Supra-Clean LCC Supra-Clean REC18 Supra-Clean REC18 SPE Strong Cation Exchange (SCX) SPE Weak Cation Exchange (WCX) Supra-Clean Mixed-Mode (MM3) Supra-Clean Strong Anion Exchange (SAX) Supra-Clean Amino (NH2-S) Supra-Clean Mixed-Mode (MM1) Supra-Clean Mixed-Mode (MM2) Supra-Clean C18 Supra-Clean 300Å C4 Supra-Clean Phenyl (PH-S) Supra-Clean Cyano (CN-S) Supra-Poly Extreme Capacity 1500 m 2 /g (XC) Supra-Clean Amino (NH2-S) Supra-Poly 1200 m 2 /g (XWP)* Supra-Poly (ATH, ATL, AEV)** Supra-Clean Florisil (FL-S) Supra-Clean Silica (SI-S) Supra-Clean Amino (NH2-S) Supra-Clean Polyamine (P6) * (XWP) = Extra Wide Particle **(ATL) = Lipophilic (AEV) = Advanced Environmental (ATH) = Hydrophobic/Hydrophilic 3
4 A Wide Array of Columns for a Broad Range of Applications. Chemistry Phase Benefit ph Range Description Load Capacity Supra-Clean (Spherical Silica) CN-S Hydrophilic 1-8 Extraction of Apolar and Mid-Polar Compounds. FL-S Hydrophilic 1-8 Extraction of Organochlorine and Polychlorinated Biphenyls. NH2-S Hydrophilic 1-8 Extraction of Apolar and Mid-Polar Compounds. P6 * Hydrophilic 1-8 Extraction of Flavones, Chalkones, Anthraquinones. SI-S Hydrophilic 1-8 Extraction of Polar Compounds. 300Å C4 Hydrophobic 1-8 Extraction of Apolar and Mid-Polar Compounds. C18 Hydrophobic 1-8 Extraction of Apolar and Mid-Polar Compounds. LCC Hydrophobic 1-8 Extraction of Apolar and Mid-Polar Compounds. PH-S Hydrophobic 1-8 Extraction of Apolar and Mid-Polar Aromatic Compounds. REC18 Hydrophobic 1-8 Extraction & Purification of Mid-Polar & Apolar compounds including 100% Water Solvents. SAX Ion Exchange 1-8 Extraction & Selective purification of acid compounds. SCX Ion Exchange 1-8 Extraction & Selective purification of basic compounds. WCX Ion Exchange 1-8 Extraction & Selective purification of strong base compounds. MM1 Mixed Mode 1-8 Extraction & Very Selective purification of basic compounds. MM2 Mixed Mode 1-8 Extraction & Very Selective purification of very basic compounds. MM3 Mixed Mode 1-8 Extraction & Very Selective purification of acidic compounds. Supra-Poly (Spherical Polymers) 0.3meq/g exchange capacity, 3mg/100mg 0.7meq/g exchange capacity, 7mg/100mg 0.22meq/g exchange capacity, 2.2mg/100mg 0.09meq/g exchange capacity, 0.9mg/100mg 0.10meq/g exchange capacity, 1.0mg/100mg 0.14meq/g exchange capacity, 1.4mg/100mg XC Extreme Capacity 0-14 Polar & non-polar compounds (XC < 3000D) Aqueous or organic solvent. High capacity due to high surface area. XWP Extreme Capacity, Wide Pore 0-14 Protein/peptide cleanup in bio-fluids. Polar and non-polar compounds. High capacity due to high surface area. ATH Hydrophilic 1-13 Mixed hydrophilic/hydrophobic interations. Mid-polar and apolar compounds in apolar and polar solvents. ATL ** Lipophilic 0-14 Excellent alternative to high flow silica for crude samples. Mid-polar & non-polar compounds (<3000D). AEV *** n.a High extraction flow of non-polar and mid-polar compounds in water. * Nylon 6 ** polystyrene-divinylbenzene *** polystyrene-co-2-hydroxyethyl methacrylate Benefits of Supra-Poly SPE. Even without a specifically designed application kit, developing methods for virtually any sample matrix or family of compounds is easy with the large number of chemistries (20+), wide ph range (1-14) and broad array of bed capacities available in PerkinElmer s Supra-Poly product line. Each column contains macro-porous polymers with ultra-pure, pharmaceutical-grade spherical particles that optimize every stage of SPE extraction. Compared with traditional silica, Supra-Poly columns offer shorter analysis times, greater load capacity, and reduced solvent usage (minimizing both procurement and disposal costs). In fact, no SPE solution is more effective at getting your samples to your instruments faster or with a greater degree of reproducibility. 4
5 Manifolds, Selection Kits and Application Packs to Streamline Your Sample Preparation. For greater method development simplicity and sample throughput, PerkinElmer offers a variety of pre-prepared kits and column manifolds. SPE Manifolds Allow you to process up to 24 samples simultaneously. Seamlessly integrate with columns to extend the system s performance and reproducibility. Feature polypropylene or stainless steel needle valves and tips with minimum dead volume. Rugged stopcocks are reusable and provide independent flow control. SPE Manifolds and Accessories Qty/pack Part Numbers Cover, Gasket, 12 Stopcocks 12 N Cover, Gasket, 24 Stopcocks 24 N Drying Attachment 12 Positions 12 N Drying Attachment 24 Positions 24 N Glass Chamber 12 Positions 12 N Glass Chamber 24 Positions 24 N Needles Polypropylene 12 Positions 12 N Needles Polypropylene 24 Positions 24 N Needles, Stainless Steel 12 Positions 12 N Needles, Stainless Steel 24 Positions 24 N Stopcocks 12 Positions 12 N Stopcocks 24 Positions 24 N Vacuum Manifold Complete Set 12 Positions 12 N Vacuum Manifold Complete Set 24 Positions 24 N SPE Selection Kits Selection kits enable quick column selection for development of reproducible and repeatable SPE methods. SPE Supra-Clean Column Selection Kits Weight/Volume Qty/pack Part Numbers Extraction of Acidic, Basic and Neutral Compounds from Aqueous or Organic Matrix 100mg/3mL 50 N Pre-Concentration of Hydrophobic Compounds from Aqueous Matrix 200mg/3mL 50 N Pre-Concentration of Hydrophobic Compounds from Aqueous Matrix 200mg/6mL 50 N Extraction of Hydrophobic Compounds from Aqueous Matrix 500mg/3mL 50 N Pre-Concentration of Hydrophilic Compounds 500mg/3mL 30 N Removal of Polar Compounds from Aqueous and Organic Matrix 500mg/3mL 30 N Extraction of Carboxylic Acids and Strong Bases from Aqueous Matrix 500mg/6mL 40 N Extraction of Hydrophobic Compounds from Aqueous Matrix 500mg/6mL 50 N Extraction of Weak Bases from Aqueous Matrix 500mg/6mL 30 N Pre-Concentration of Hydrophilic Compounds 500mg/6mL 30 N Removal of Polar Compounds from Aqueous and Organic Matrix 500mg/6mL 30 N SPE Application Packs Ideal for extraction of known entities from a variety of matrices, our packs are expertly tailored to meet your application needs and are designed to support major EPA methods and applications. SPE Supra-Clean Application Packs Weight/Volume Qty/pack Part Numbers Extraction of PAH from Soil and Oil 1.5g/6mL 30 N Extraction of PAH from Soil and Oil (Glass Straight Column) 1.5g/6mL 30 N Extraction of PAH from Water Containing Humic Acids 1.5g/6mL 30 N Extraction of PCB from Oil 1g/3mL 50 N Extraction of Bisphenol A from Aqueous Matrix 1g/6mL 30 N Extraction of Oil and Grease from Aqueous Matrix-EPA g/6mL 30 N Extraction of PCB from Oil 1g/6mL 30 N Extraction of SVOC from Water-EPA 525 1g/6mL 30 N Extraction of Basic Drugs from Biological Fluids 200mg/3mL 50 N Extraction of PAH from Water or Soil 4g/6mL 30 N Extraction of PAH from Water or Soil (Glass Straight Column) 4g/6mL 30 N Extraction of Oil and Grease from Aqueous Matrix-EPA mg/3mL 50 N Extraction of Pesticides and Herbicides from Aqueous Matrix 500mg/6mL 50 N Extraction of Steroids from Biological Fluids 500mg/6mL 50 N
6 Complete Consistency. Complete Confidence. Weight/Volume Qty/pack Part Number SPE Supra-Clean Cyano (CN-S) Column 500mg/3mL 50 N mg/6mL 30 N SPE Supra-Clean Florisil (FL-S) Column 1g/6mL 30 N mg/3mL 50 N g/15mL 20 N g/25mL 20 N g/6mL 20 N mg/3mL 50 N mg/6mL 30 N SPE Supra-Clean Florisil (FL-S) Pesticide Grade Column 1g/6mL 30 N mg/3mL 50 N g/15mL 20 N g/25mL 20 N g/6mL 20 N mg/3mL 50 N mg/6mL 30 N SPE Supra-Clean Amino (NH2-S) Column 100mg/1mL 100 N mg/3mL 50 N mg/3mL 50 N mg/3mL 50 N mg/6mL 30 N mg/1mL 50 N SPE Supra-Clean Polyamine (P6) Column 500mg/3mL 50 N mg/6mL 30 N Weight/Volume Qty/pack Part Number SPE Supra-Clean 300Å C4 Column 100mg/1mL 100 N mg/3mL 50 N mg/3mL 50 N mg/1mL 50 N SPE Supra-Clean C18 Column 100mg/1mL 100 N mg/3mL 50 N g/6mL 30 N mg/3mL 50 N g/15mL 20 N g/25mL 20 N g/6mL 20 N mg/3mL 50 N mg/6mL 30 N mg/1mL 50 N mg/6mL 30 N mg/6mL 30 N mg/3mL 50 N SPE Supra-Clean LCC Column 500mg/3mL 50 N mg/6mL 30 N SPE Supra-Clean Phenyl (PH-S) Column 100mg/1mL 100 N mg/3mL 50 N g/6mL 30 N mg/3mL 50 N mg/3mL 50 N mg/6mL 30 N mg/1mL 50 N SPE Supra-Clean Silica (SI-S) Column 100mg/3mL 50 N g/6mL 30 N mg/3mL 50 N g/15mL 20 N g/25mL 20 N g/6mL 20 N mg/3mL 50 N mg/6mL 30 N SPE Supra-Clean REC18 Column (full accessible surface) 100mg/1mL 100 N mg/3mL 50 N g/6mL 30 N mg/3mL 50 N mg/3mL 50 N mg/6mL 30 N mg/1mL 50 N mg/1mL 30 N
7 Weight/Volume Qty/pack Part Number Weight/Volume Qty/pack Part Number SPE Supra-Clean Strong Anion Exchange (SAX) Column 100mg/1mL 100 N mg/3mL 50 N mg/3mL 50 N mg/3mL 50 N mg/6mL 30 N mg/1mL 50 N SPE Supra-Clean Strong Cation Exchange (SCX) Column 100mg/1mL 100 N mg/3mL 50 N mg/3mL 50 N mg/3mL 50 N mg/6mL 30 N mg/1mL 50 N SPE Supra-Clean Weak Cation Exchange (WCX) Column 100mg/1mL 100 N mg/3mL 50 N mg/3mL 50 N mg/3mL 50 N mg/6mL 30 N mg/1mL 50 N SPE Supra-Clean Mixed-Mode (MM1, MM2, MM3) Column 100mg/1mL 100 N (MM1) 100mg/3mL 50 N (MM1) 200mg/3mL 50 N (MM1) 500mg/3mL 50 N (MM1) 500mg/6mL 30 N (MM1) 50mg/1mL 50 N (MM1) 100mg/1mL 100 N (MM2) 100mg/3mL 50 N (MM2) 200mg/3mL 50 N (MM2) 500mg/3mL 50 N (MM2) 500mg/6mL 30 N (MM2) 50mg/1mL 50 N (MM2) 500mg/6mL 30 N (MM3) For more information or to experience the unparalleled consistency of PerkinElmer SPE solutions, please contact your sales representative, call or visit SPE Supra-Poly Extreme Capacity 1500 m 2 /g (XC) Column 100mg/1mL 50 N mg/3mL 50 N g/15mL 20 N mg/3mL 50 N mg/1mL 50 N mg/6mL 30 N mg/1mL 50 N mg/1mL 50 N mg/3mL 50 N mg/6mL 30 N SPE Supra-Poly Extra Wide Particle 1200 m 2 /g (XWP) Column 100mg/1mL 50 N mg/3mL 50 N g/15mL 20 N mg/3mL 50 N mg/1mL 50 N mg/6mL 30 N mg/1mL 50 N mg/1mL 50 N mg/3mL 50 N SPE Supra-Poly ATH, ATL, AEV Polymer Column 200mg/6mL 50 N (ATH) 200mg/3mL 50 N (ATH) 100mg/3mL 50 N (ATH) 200mg/6mL 50 N (ATL) 200mg/3mL 50 N (ATL) 100mg/3mL 50 N (ATL) 100mg/3mL 50 N (AEV) SPE Clean Silica (SI-S) Cartridge (full accessible surface) 1300mg 50 N mg 50 N mg 50 N SPE Supra-Clean C18 Cartridge (full accessible surface) 1690mg 50 N mg 50 N mg 50 N
8 At PerkinElmer, we understand that sample preparation is one of the most critical steps in the analytical process. Often accounting for 60% of your timetable, it has a fundamental impact on a wide range of operational parameters. Any errors within this process undermine the quality of your data at all subsequent stages of your analysis. Solid Phase Extraction helps avoid potential errors in sample preparation, reducing re-runs and dramatically increasing productivity. As one of the most cost-effective and flexible tools within the laboratory environment, SPE also provides efficient sample concentration and purification prior to many of today s most popular analytical techniques, including HPLC, LC/MS, GC and GC/MS. The right accessories, consumables, methods and application support are as integral to the success of your laboratory as your instrumentation. That s why we invest heavily in testing and validating our complete portfolio of solutions to ensure you receive accurate, repeatable results on time, every time. SPE columns are part of PerkinElmer s complete offering of analytical solutions an industry-leading portfolio that encompasses instruments, accessories, consumables, supplies, training and service. It s a breadth of capabilities that enables us to offer the ease and convenience of having a single supplier for all your needs at every stage of your workflow. So you can benefit from greater responsiveness, superior reliability, and dramatic cost savings. Couple SPE With Leading Instrumentation For Ultimate Laboratory Efficiency Clarus 680/580/480 GC Clarus SQ 8 GC/MS Flexar SQ 300 MS Flexar LC and UHPLC Our OneSource Laboratory Services division even takes it a step further. With more than 1,500 trained and certified field service engineers and service personnel around the world, OneSource offers the most comprehensive portfolio of professional laboratory services in the industry, including complete care programs for virtually every technology and manufacturer. So turn to PerkinElmer. For the experience. For the confidence. For the better. PerkinElmer, Inc. 940 Winter Street Waltham, MA USA P: (800) or (+1) For a complete listing of our global offices, visit Copyright 2011, PerkinElmer, Inc. All rights reserved. PerkinElmer is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners _01 Printed in USA
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