STUDIES ON DATE PALM IN TISSUE CULTURE
|
|
- Sheena Gaines
- 5 years ago
- Views:
Transcription
1 STUDIES ON DATE PALM IN TISSUE CULTURE By GEHAN MOHAMED YOUSRY SALAMA B.Sc. Agric. Sci. (Horticulture), Fac. Agric., Ain Shams Univ., 1990 M.Sc. Agric. Sci. (Ornamental Horticulture), Fac. Agric., Cairo Univ., 2004 THESIS Submitted in Partial Fulfillment of the Requirements for the Degree of DOCTOR OF PHILOSOPHY In Agricultural Sciences (Ornamental Horticulture) Department of Ornamental Horticulture Faculty of Agriculture Cairo University EGYPT 2012
2
3 APPROVAL SHEET STUDIES ON DATE PALM IN TISSUE CULTURE Ph.D. Thesis In Agric. Sci. (Ornamental Horticulture) By GEHAN MOHAMED YOUSRY SALAMA B.Sc. Agric. Sci. (Horticulture), Fac. Agric., Ain Shams Univ., 1990 M.Sc. Agric. Sci. (Ornamental Horticulture), Fac. Agric., Cairo Univ., 2004 APPROVAL COMMITTEE Dr. HUSSEIN ABD EL HAQ BOSILA... Professor of Floriculture, Medicinal and Aromatic Plants, Fac. Agric., Al- Azhar University, Cairo. Dr. MOHAMED ABDEL-KHALIK AL KHATEEB... Professor of Ornamental Horticulture, Fac. Agric., Cairo University Dr. AFAF MAHMOUD HABIB... Associate Professor of Ornamental Horticulture, Fac. Agric., Cairo University Dr. EL- SAADY MOHAMED BADAWY... Professor of Ornamental Horticulture, Fac. Agric., Cairo University Date: 20 / 11 / 2012
4
5 SUPERVISION SHEET STUDIES ON DATE PALM IN TISSUE CULTURE Ph.D. Thesis In Agric. Sci. (Ornamental Horticulture) By GEHAN MOHAMED YOUSRY SALAMA B.Sc. Agric. Sci. (Horticulture), Fac. Agric., Ain Shams Univ., 1990 M.Sc. Agric. Sci. (Ornamental Horticulture), Fac. Agric., Cairo Univ., 2004 SUPERVISION COMMITTEE Dr. EL- SAADY MOHAMED BADAWY Professor of Ornamental Horticulture, Fac. Agric., Cairo University Dr. AFAF MAHMOUD HABIB Associate Professor of Ornamental Horticulture, Fac. Agric., Cairo University Dr. ABDEL-MONEM EL-BANA Professor of Date palm, Director of Agric. Research Center, Giza
6
7 Name of Candidate: Gehan Mohamed Yousry Salama Degree: Ph.D. Title of Thesis: Studies on Date Palm in Tissue Culture Supervisors: Dr. EL- Saady Mohamed Badawy Dr. Afaf Mahmoud Habib Dr. Abdel- Monem El- Bana Department: Ornamental Horticulture Approval: 20 / 11 / 2012 ABSTRACT This study had been carried out at the Ornamental Horticulture, Department, Faculty of Agriculture, Cairo University, during the period from 2007 to The experiments had been conducted in the Centre Laboratory for Date Palm Research, Plant Biotechnology Department, Agriculture Research Center, Ministry of Agriculture, Giza, Egypt. The aim of this study was to investigate a suitable protocol to improve the production and development of somatic embryos of date palm cv. Sakkoty, through suspension culture technique and to indentify appropriate conditions for protoplast isolation and fusion between Sakkoty and Khalas cv. Studying the factors affecting growth and development of friable calli induced from shoot tip explants including mesh filter size, 2,4-D, vitamins, amino acids and thidiazurone (TDZ) at different concentrations, in separate experiments. The obtained results revealed that globularization degree and somatic embryo number were enhanced using filter mesh size at 500 ϻ M especially in combination with liquid medium enriched with 0.1 mg/l 2,4-D. Thiamin HCl at 2.5 mg/l was superior in enhancing somatic embryo formation than other tested vitamins when added to MS liquid medium mg/l 2,4-D mg/l NAA. Glutamine had stimulatory effects on cell suspension culture of date palm compared with asparagine and argenine. The addition of TDZ to suspension culture medium at 1.0 mg/l promoted somatic embryo production. Biochemical components varied according to different developmental stages. Globular stage had a highest significant content of total soluble sugars, while cotyledonary stage contained high phenols content. No significant differences in free amino acids and indoles were observed through different stages. Enzyme concentrations and incubation time affected positively on protoplasts yield. The best yield was obtained from addition of cellulose 1.5 %, hemicellulase 0.5 % and Macerozyme at 0.5 % and incubation time for 12 hours. The effects of ABA, GA 3, sucrose different concentrations, temperature degree during maturation and germination stages and histological studies during callus growth and development were investigated. Key words: Date palm, somatic embryogenesis, callus, 2,4-D, biotin, TDZ, Temperatures, GA 3, ABA, cell suspension and protoplast.
8
9 ACKNOWLEDGEMENT Thanks to ALLAH I would like to express my sincere gratitude and admiration to my teacher Prof. Dr. EL- SAADY MOHAMED BADAWY Professor of Ornamental Horticulture, Faculty of Agriculture, Cairo University for suggesting the problem; his supervision teaching, support and patience. I am particularly grateful to him for his valuable instructions during carrying out of the experiments and correction of the manuscript. I have been greatly assisted by Dr. AFAF MAHMOUD HABIB Associate Professor of Ornamental Horticulture, Faculty of Agriculture, Cairo University, is thanks for her supervision, support and advice during my studies. Prof. Dr. Abdel-Monem El-Bana Head of Research of Date palm Lab., ARC, Giza is acknowledged for his supervision and providing excellent library facilities at the date palm Research Lab. Sincere thanks are extended to Dr. Ahmed Gabr (National Research Center-Cairo, Egypt) for his advice and careful help in protoplast fusion work, I would like to express my deepest thanks and sincere appreciation to Prof. Dr. Mona Hassan,Dr. Amal Fathy, Dr. Rehab Sidky and Dr. Eman Mostafa Researcher in the Date Palm Tissue Culture Lab for their advice helping in the laboratory work. Also great thanks to Dr. Mohamed Abd El-Rasool for his helping in the histological work. Thanks too, to all staff members of Ornamental Horticulture, Department, Faculty of Agriculture, Cairo University. Thanks go out to all members of the Date Palm Lab. for their friendly advice, support and unlimited help during these years. Sincere thanks and gratitude to my parent s soul, husband and sister for encouragement and providing all the facilities throughout this work.
10
11 CONTENTS Page INTRODUCTION... 1 REVIEW OF LITERATURE Establishment of callus suspension cultures... 7 a. Plant materials... 7 b. Sterilization... 8 c. Callus induction Auxin and cytokinin Somatic embryos maturation High frequency and synchronous somatic embryogenesis a. Cell suspensions b. Effect of vitamins c. Effect of amino acids d. Effect of thidiazurone e. Effect of abscissic acid f. Effect of gibberellic acid g. Effect of sucrose h. Effect of temperature degrees Biochemical and structural analyses Protoplast induction, culture and fusion MATERIALS AND METHODS Establishment of calli cultures Maturation stage a. Effect of different mesh filter diameters (callus sizes) and 2,4-D concentrations b. Effect of vitamin concentrations and culture media... c. Effect of different concentrations of amino acid d. Effect of different concentrations of 2,4-D and TDZ on date palm suspensions cultures e. Effect of incubation under different temperature degrees Germination stage I 46 48
12
13 4. Rooting and Pre-acclimatization Extraction and determination of carbohydrate components and free amino acid a. Extraction b. Determination of total soluble sugars c. Determination of reducing sugars d. Determination of free amino acids Extraction and determination of total phenols and indoles a. Determination of total phenols b. Determination of total indoles Histological studies protoplast isolation a. Plant materials b. Protoplast isolation c. Purification of protoplast d. Protoplast culture e. Protoplast fusion and culture RESULTS AND DISCUSSION Maturation stage a. Effect of 2,4-D concentrations and mesh filter diameter of callus suspensions cultures on 1.Globularization degree Number of somatic embryos b. Effect of vitamins and culture media on 1.Globulrization degree Number of somatic embryos formed from embryogenic callus cultured in three media c. Effect of different concentrations of amino acids on growth and development of date palm callus suspension culture Experiment Effect of 2,4-D and TDZ concentrations on growth and development of date palm callus suspension culture a. growth value b. Globularization degree II
14
15 c. Number of somatic embryos d. Vitrification Experiment Effect of ABA and temperatures on growth and development of date palm during maturation and germination stage Maturation stage a. Growth value b. Globularization degree c. Number of somatic embryos d. Vitrification Germination stage a. Number of secondary somatic embryos b. Number of germinated embryos c. Number of roots/embryo d. Vitrification degree Effect of GA 3 and temperatures on growth and development of date palm during maturation and germination stage Maturation stage a. Growth value b. Globularization degree c. Number of somatic embryos d. Vitrification Germination stage a. Number of secondary somatic embryos b. Germinated embryos c. Number of roots/ embryo d. Vitrification Effect of sucrose and temperatures on growth and development of date palm during maturation and germination stage Maturation stage a. Growth value b. Globularization degree III
16
17 c. Number of somatic embryos d. Vitrification Germination stage a. Number of secondary somatic embryos b. Germinated embryos c. Number of roots/embryo d. Vitrification Rooting and Pre-acclimatization Biochemical components carbohydrate components Free amino acids, indoles and phenols components a. Free amino acids b. Indoles c. Phenols Histological studies Protoplasts isolation, culture and fusion of two date palm cultivars cv. Sakkoty and Khalas Protoplasts isolation Protoplasts culture Protoplasts fusion SUMMARY REFERENCES ARABIC SUMMARY... IV
18
19 No LIST OF TABLES Title Mixture of enzyme solutions used in protoplast isolation from embryogenic calli of date palm cultivars Sakkoty and Khalas... Composition of incubation mixture (CPW)... Modified Kao and Michayluk medium (1975)... Effect of 2,4-D concentrations and mesh filter diameter of callus suspensions on globularization degree of somatic Effect of 2,4-D concentrations and mesh filter diameter of callus suspension on number of somatic embryos Effect of vitamins and culture media on globularization degree of callus... Effect of vitamins and culture media on number of somatic embryos formed from embryogenic callus cultured in three media... Effect of different concentrations of amino acid on growth and development of date palm callus suspension... Effect of 2,4-D and TDZ concentrations on growth and development of date palm callus suspension... Effect of abscisic acid and temperatures on growth and development of date palm callus suspension... Effect of abscisic acid and temperature on number of secondary somatic embryos, germinated embryos, number of roots and vitrification... Effect of gibberellic acid and temperature on growth and development of date palm callus suspension... Effect of gibberellic acid and temperature on number of secondary somatic embryos, germinated embryos, number of roots and vitrification... Page V
20
Plantlet Regeneration via Somatic Embryogenesis in Four Species of Crocus
Plantlet Regeneration via Somatic Embryogenesis in Four Species of Crocus Roya Karamian Department of Biology, Faculty of Science Bu-Ali Sina University Hamadan Iran Keywords: meristems, micropropagation,
More informationInternational Journal of Pharma and Bio Sciences DIRECT REGENERATION FROM EMBRYO CULTURES OF LYCOPERSICON ESCULENTUM MILL CV PUSA RUBY ABSTRACT
Research Article Plant science International Journal of Pharma and Bio Sciences ISSN 0975-6299 DIRECT REGENERATION FROM EMBRYO CULTURES OF LYCOPERSICON ESCULENTUM MILL CV PUSA RUBY P. KARUNAKAR RAO Department
More informationIn Vitro Microcorm Formation in Saffron (Crocus sativus L.)
In Vitro Microcorm Formation in Saffron (Crocus sativus L.) W. Raja, G. Zaffer, S.A. Wani Division of Plant Breeding & Genetics Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir
More informationMICROPROPAGATION OF RICE (ORYZA SATIVA L. CV SWAT-II) THROUGH SOMATIC EMBRYOGENESIS
Pak. J. Bot., 37(2): 237-242, 2005. MICROPROPAGATION OF RICE (ORYZA SATIVA L. CV SWAT-II) THROUGH SOMATIC EMBRYOGENESIS IHSAN ILAHI, SHAZIA BANO, MUSARRAT JABEEN AND FAZAL RAHIM Department of Botany, University
More informationUNIVERSITI TEKNOLOGI MARA. IN VITRO REGENERATION, ACCLIMATIZATIONAND FRUIT QUALITY ASSESSMENTS OF ROCKMELON (Cucumis melo) CV.
UNIVERSITI TEKNOLOGI MARA IN VITRO REGENERATION, ACCLIMATIZATIONAND FRUIT QUALITY ASSESSMENTS OF ROCKMELON (Cucumis melo) CV. GLAMOUR SAIYIDAH NAFISAH BINTI HASHIM Thesis submitted in fulfillment of the
More informationFigure 6. The type of oil palm explants used in these experiments. A. Leaf explant, B. Zygotic embryos explant, and C. Female flower explant
22 MATERIALS AND METHODS Location and Time This work was carried out in the Laboratory of Plant Biotechnology and Tissue Culture, Faculty of Agriculture, Bogor Agricultural University. It was started from
More informationSOMATIC EMBRYOGENESIS AND REGENERATION OF PLANTLET IN SAFFRON, CROCUS SATIVUS L.
J. Sci. I. R. Iran Vol. 11, No. 3, Summer 2000 SOMATIC EMBRYOGENESIS AND REGENERATION OF PLANTLET IN SAFFRON, CROCUS SATIVUS L. H. Ebrahimzadeh 1*, R. Karamian 2 and M. R. Noori-Daloii 3 1 Department of
More informationapplication in propagating Butterfly attracting Plants
Plant Tissue Culture and its application in propagating Butterfly attracting Plants Kodiswaran Kandasamy Tissue Culture Unit Forest Biotechnology Division FRIM kodiswaran@frim.gov.my Cratoxylon formosum
More informationThe preferred carbohydrate in plant cell culture media is sucrose. Glucose and fructose may be substituted in some cases, glucose being as
Introduction One of the most important factors governing the growth and morphogenesis of plant tissues in culture is the composition of the culture medium. The basic nutrient requirements of cultured plant
More informationTitle: Development of Micropropagation and Acclimation Protocols for the Commercialization of a New Bonsai Ornamaental Tree for the California Market.
Title: Development of Micropropagation and Acclimation Protocols for the Commercialization of a New Bonsai Ornamaental Tree for the California Market. Authors: Dan E. Parfitt 1, Helen M. Chan 2, and Ali
More informationSOMATIC EMBRYOGENESIS OF DREPANOSTACHYUM FALCATUM AN IMPORTANT HILL BAMBOO-A RAPID MEANS OF MICROPROPAGATION
SOMATIC EMBRYOGENESIS OF DREPANOSTACHYUM FALCATUM AN IMPORTANT HILL BAMBOO-A RAPID MEANS OF MICROPROPAGATION I.D.Arya, R. Sharma & Sarita Arya Forest Genetics & Tree Propagation Division, Arid Forest Research
More informationPlant Tissue Culture. Dr. Alain Lemansour UAE University Date Palm Development Research Unit Dept.
Plant Tissue Culture By Dr. Alain Lemansour UAE University Date Palm Development Research Unit Dept. What is it? Tissue culture is the term used for the process of growing cells artificially in the laboratory
More informationDEPARTMENT OF BOTANY Guru Ghasidas Vishwavidyalaya, Bilaspur B. Sc. V Semester LBC 503 (Plant Tissue Culture)
DEPARTMENT OF BOTANY Guru Ghasidas Vishwavidyalaya, Bilaspur B. Sc. V Semester LBC 503 (Plant Tissue Culture) Section A Multiple choice questions / Define the following terms 1 10 = 10 1. (d) Agrobacterium
More informationIN VITRO PROPAGATION OF EGYPTIAN DATE PALM: 11-DIRECT AND INDIRECT SHOOT PROLIFERATION FROM SHOOT-TIP EXPLANTS OF Phoenix dactylifera L. CV. ZAGHLOOL.
IN VITRO PROPAGATION OF EGYPTIAN DATE PALM: 11-DIRECT AND INDIRECT SHOOT PROLIFERATION FROM SHOOT-TIP EXPLANTS OF Phoenix dactylifera L. CV. ZAGHLOOL. Shawky A. Bekheet and Mahmoud M. Saker Plant Cell
More informationCurriculum Vitae. Personal information:
Curriculum Vitae Personal information: Name: Mohamed Mohamed El-Mogy Date of birth: 5/6/1975 Place of birth: Giza, Egypt. Nationality: Egyptian Gender: Male Current position: Associate Professor at Cairo
More informationInvestigation of Plant Growth Regulators Effects on Callus Induction and Shoot Regeneration of Bunium persicum (Boiss.) B. Fedtsch.
J. Agr. Sci. Tech. (2009) Vol. 11: 481-486 Investigation of Plant Growth Regulators Effects on Callus Induction and Shoot Regeneration of Bunium persicum (Boiss.) B. Fedtsch. M. Valizadeh 1, and S. K.
More informationChapter 1 Introduction
Chapter 1 Introduction Experimental systems based on plant cell and tissue culture are characterized by the use of isolated parts of, called ex, obtained from an intact plant body and kept on, or in a
More informationIntroduction. Plant growth regulators are the critical media components in determining the developmental pathway of the plant cells.
Introduction Callus is defined as an unorganized tissue mass growing on solid substrate. Callus forms naturally on plants in response to wounding, infestations, or at graft unions (Bottino, 1981). Callus
More informationBioscience Research Print ISSN: Online ISSN:
Available online freely at www.isisn.org Bioscience Research Print ISSN: 1811-9506 Online ISSN: 2218-3973 Journal by Innovative Scientific Information & Services Network RESEARCH ARTICLE BIOSCIENCE RESEARCH,
More informationPlant Regeneration via Organogenesis and Somatic Embryogenesis
18 Plant Biotechnology: Practical Manual 2 Plant Regeneration via Organogenesis and Somatic Embryogenesis 2.1 BACKGROUND AND BASICS One can achieve plant regeneration in a test tube by using suitable culture
More informationImproving in vitro propagation of Protea cynaroides L. (King Protea) and the roles of starch and phenolic compounds in the rooting of cuttings
Improving in vitro propagation of Protea cynaroides L. (King Protea) and the roles of starch and phenolic compounds in the rooting of cuttings by How-Chiun Wu Submitted in partial fulfillment of the requirements
More informationHigh Frequency Shoots Regeneration from Cotyledon Explants of Teasle Gourd Via Organogenesis
High Frequency Shoots Regeneration from Cotyledon Explants of Teasle Gourd Via Organogenesis Mohammad Abdul Karim Dept. of Crop Botany, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh2202,
More informationIN VITRO PROPAGATION OF THE BALKAN ENDEMIC SPECIES VERBASCUM ERIOPHORUM GODR. Abstract. Introduction
767 Bulgarian Journal of Agricultural Science, 22 (No 5) 2016, 767 771 Agricultural Academy IN VITRO PROPAGATION OF THE BALKAN ENDEMIC SPECIES VERBASCUM ERIOPHORUM GODR. Zh. P. YORDANOVA *, M. A. ROGOVA
More informationIN VITRO CONSERVATION OF DATE PALM SHOOT TIP EXPLANTS UNDER MINIMAL GROWTH CONDITION
Egypt. J. Agric. Res., 91(3),2013 1043 IN VITRO CONSERVATION OF DATE PALM SHOOT TIP EXPLANTS UNDER MINIMAL GROWTH CONDITION EL-DAWAYATI, MAIADA M. 1, EL- I. BAKER 2, AMINA H. GOMAA 2 AND ZEINAB E. ZAYED
More informationIsolation of Protoplasts from Suspension Culture and Subsequent Shoot Regeneration in Sugarcane
JARQ 30, 21-26 (1996) Isolation of Protoplasts from Suspension Culture and Subsequent Shoot Regeneration in Sugarcane Makoto MATSUOKA*l, Takayoshi TERAUCHI*2, Makoto KOBAYASHI*2, Moriyuki SHODA *3 and
More informationORGANOGENESIS IN CHRYSANTHEMUM MORIFOLIUM RAMAT (CULTIVAR ROMICA ) CALLUS CULTURES SMARANDA VÂNTU
Analele ştiinţifice ale Universităţii Al. I. Cuza Iaşi Tomul LII, s. II a. Biologie vegetală, 006 ORGANOGENESIS IN CHRYSANTHEMUM MORIFOLIUM RAMAT (CULTIVAR ROMICA ) CALLUS CULTURES SMARANDA VÂNTU Abstract:
More informationIn Vitro Regeneration of Papaya (Carica papaya L.) Variety Surya
Available online at www.ijpab.com DOI: http://dx.doi.org/10.18782/2320-7051.6269 ISSN: 2320 7051 Int. J. Pure App. Biosci. 6 (4): 456-461 (2018) Research Article In Vitro Regeneration of Papaya (Carica
More informationGregor Mendel Foundation Proceedings 2007:
Gregor Mendel Foundation Proceedings 2007: 54-61. Effect of the explants from different aged mother plant on callus induction and direct regeneration in jatropha(jatropha curcas L.) Pranesh K. J 1, Gururaja
More informationIPC TECHNICAL PAPER SERIES NUMBER 263
THE INSTITUTE OF PAPER CHEMISTRY, APPLETON, WISCONSIN IPC TECHNICAL PAPER SERIES NUMBER 263 FACTORS INFLUENCING THE INITIATION OF SOMATIC EMBRYOGENESIS IN PINUS STROBUS L. S. R. WANN, M. R. BECWAR, L.
More informationComparison of Regeneration Efficiency of Three Different Genotypes of Basmati Rice under in Vitro Condition
Comparison of Regeneration Efficiency of Three Different Genotypes of Basmati Rice under in Vitro Condition Ritu Mahajan* and Shilpi Sharma School of Biotechnology, University of Jammu, Jammu, India Abstract
More informationAGRONOMICAL AND PHYSIOLOGICAL FACTORS AFFECTING GROWTH, DEVELOPMENT AND YIELD OF SWEET POTATO IN ETHIOPIA
AGRONOMICAL AND PHYSIOLOGICAL FACTORS AFFECTING GROWTH, DEVELOPMENT AND YIELD OF SWEET POTATO IN ETHIOPIA by Terefe Belehu Submitted in partial fulfilment of the requirements of Doctor of Philosophy Department
More informationEffect of Cytokinins on Multiple Shoot Regeneration from Leaf Derived Callus of Inula
Plant Tissue Cult. & Biotech. 27(2): 189 194, 2017 (December) PTC&B Effect of Cytokinins on Multiple Shoot Regeneration from Leaf Derived Callus of Inula royleana DC. Samar Amin*, Zahoor A Kaloo and Seema
More informationUse of Tissue culture to alleviate the supply of quality planting materials among cacao smallholder farmers in Cote d Ivoire
Use of Tissue culture to alleviate the supply of quality planting materials among cacao smallholder farmers in Cote d Ivoire Jane Kahia, Siaka Kone, Georges Ngoran, Lucien Diby.,Jean Claude Nzi and Christophe
More informationIn Vitro Multiplication Of Date Palm. Iqbal Hussain, Hamid Rashid, Aish Muhammad & Azra Quraishi
In Vitro Multiplication Of Date Palm Iqbal Hussain, Hamid Rashid, Aish Muhammad & Azra Quraishi Agricultural Biotechnology Institute, National Agriculture Research Center, Islamabad, Pakistan. ABSTRACT
More informationEFFECT OF GROWING MEDIA, ORGANIC AND BIOFERTILIZERS ON GROWTH AND FLOWERING OF FREESIA REFRACTA CV. RED LION
EFFECT OF GROWING MEDIA, ORGANIC AND BIOFERTILIZERS ON GROWTH AND FLOWERING OF FREESIA REFRACTA CV. RED LION By DEYAA ISLAM ATOWA B.Sc. Agric. Sci. (Ornamental Horticulture), Fac. Agric., Cairo Univ.,
More informationAn efficient protocol for somatic embryogenesis of garlic (Allium sativum L.) using root tip as explant
J. Bangladesh Agril. Univ. 12(1): 1 6, 2014 ISSN 1810-3030 An efficient protocol for somatic embryogenesis of garlic (Allium sativum L.) using root tip as explant M. N. Hassan 1*, M. S. Haque 2, M. M.
More informationEFFECT OF BENZYLAMINO PURINE AND NAPHTHALENE ACETIC ACID ON CALLUS AND PROTOCORM FORMATION OF DENDROBIUM CV. BANYAT PINK
Journal of Cell and Tissue Research Vol. 13(3) 3977-3981 (2013) (Available online at www.tcrjournals.com) ISSN: 0973-0028; E-ISSN: 0974-0910 Original Article EFFECT OF BENZYLAMINO PURINE AND NAPHTHALENE
More informationUNIVERSITI TEKNOLOGI MARA IMPLICATION OF NIGHT-TIME LEISURE ACTIVITIES TOWARDS PLACE IDENTITY OF URBAN PUBLIC PARK IN SHAH ALAM AND PUTRAJAYA
UNIVERSITI TEKNOLOGI MARA IMPLICATION OF NIGHT-TIME LEISURE ACTIVITIES TOWARDS PLACE IDENTITY OF URBAN PUBLIC PARK IN SHAH ALAM AND PUTRAJAYA MOHD RIDUAN BIN NGESAN Thesis submitted in fulfilment of the
More informationEFFECTIVE CALLUS INDUCTION AND PLANT REGENERATION IN BRASSICA NAPUS (L.) VAR DGS-1
Journal of Cell and Tissue Research Vol. 12(2) 3229-3234 (2012) ISSN: 0974-0910 (Available online at www.tcrjournals.com) Original Article EFFECTIVE CALLUS INDUCTION AND PLANT REGENERATION IN BRASSICA
More informationAmerican-Eurasian Journal of Sustainable Agriculture, 6(4): , 2012 ISSN Somatic Embryogenesis and Plantlet Regeneration in Amla
American-Eurasian Journal of Sustainable Agriculture, 6(4): 417-421, 212 ISSN 1995-748 417 ORIGINAL ARTICLE Somatic Embryogenesis and Plantlet Regeneration in Amla L. Al-Sabah, C. Sudhersan and S. Jibi
More informationLandscape construction processes through a quality management system
Landscape construction processes through a quality management system BY: Hesham Ibrahim Mohammed Hendawy A thesis submitted to the Faculty of Engineering at Cairo University In Partial Fulfillment of the
More informationNational Science Foundation Plant Genome Cereal Plant Transformation Workshop Albert Kausch University of Rhode Island
National Science Foundation Plant Genome Cereal Plant Transformation Workshop Albert Kausch University of Rhode Island Rice Transformation NSF Plant Transformation Workshop Albert Kausch University of
More informationCENTRE OF STUDIES FOR BUILDING SURVEYING FACULTY OF ARCHITECTURE, PLANNING AND SURVEYING UNIVERSITI TEKNOLOGI MARA
CENTRE OF STUDIES FOR BUILDING SURVEYING FACULTY OF ARCHITECTURE, PLANNING AND SURVEYING UNIVERSITI TEKNOLOGI MARA A STUDY DESIGN AND MAINTENANCE OF FACADE FOR GOVERNMENT BUILDING, PUTRAJAYA SAHRIZAN BIN
More informationCallus Induction and Regeneration using Different Explants of Sorghum
Callus Induction and Regeneration using Different Explants of Sorghum Intrduction In recent years, biotechnology is emerging as one of the latest tools of agricultural research. One of the areas of plant
More informationFACILE PLANT REGENERATION FROM TOMATO LEAVES INDUCED WITH SPECTINOMYCIN
Pak. J. Bot., 38(4): 947-952, 2006. FACILE PLANT REGENERATION FROM TOMATO LEAVES INDUCED WITH SPECTINOMYCIN MUHAMMAD SARWAR KHAN *, MUHAMMAD USMAN AND MUHAMMAD ILYAS LILLA National Institute for Biotechnology
More informationPlant regeneration from cultured protoplasts of a glutinous rice
Plant regeneration from cultured protoplasts of a glutinous rice Cell Research (1990), 1, 17-21 Wang Guangyuan, Hsia Chenau(Xia Zhenao), Chi Jinfen and Gao Xiaoyan Shanghai Institute of Plant Physiology,
More informationPolysiphonia. Life cycle of Polysiphonia Dr. Purnima Saikia, Asst.Prof Dept of Botany2017
Polysiphonia Life cycle of Polysiphonia Dr. Purnima Saikia, Asst.Prof Dept of Botany2017 Polysiphonia thallus Development of female gametophyte MALE SEX ORGANS Cystocarp with carposporangium Mature Cystocarp
More informationOriginal Papers Plant Tissue Culture Letters, 11(3), (1994) INouE**, Koji NoMuRA***, Seiko TAKAHASHi**, OsHIMA* and Kiyoshi MASUDA**
Original Papers Plant Tissue Culture Letters, 11(3), 184-190 (1994) An Efficient Procedure for Plant Regeneration from Long-term-cultured Callus Tissue of Hybrid Poplar (Populus sieboldii Miq. X Populus
More informationAppropriating the New Urbanism Principles to the Egyptian Context
Appropriating the New Urbanism Principles to the Egyptian Context Thesis Submitted For the Partial Fulfillment of the Master of Science Degree in Urban Planning Submitted by Ayat Ismail Abdel Fattah Ismail
More informationSK. Jaffar * et al. /International Journal Of Pharmacy&Technology
ISSN: 0975-766X CODEN: IJPTFI Available Online through Research Article www.ijptonline.com INVITRO REGENERATION OF BANANA (MUSA SPP.) M. Guru Prasad 1, SK. Jaffar* 2, Ch. Manjula 3 Regional Agriculture
More informationUsama Abd El-Raouf Mostafa BADAWI
Usama Abd El-Raouf Mostafa BADAWI Assistant Professor and Chair, Department of Management Information Systems Personal Data Nationality Egyptian Date of Birth 1-1-1970 Department Management Information
More informationEffect of BA NAA and 2,4-D on Micropropagation of Jiaogulan (Gynostemma pentaphyllum Makino)
2012 International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies. International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies http://tuengr.com,
More informationChapter 4. In vitro callus multiplication, regeneration and microcorm induction in Amorphophallus paeoniifolius
Chapter 4 70 Chapter 4 In vitro callus multiplication, regeneration and microcorm induction in Amorphophallus paeoniifolius 4.1 Introduction The high incidence of mosaic disease, corm dormancy and non-availability
More informationGENERATION AND SELECTION OF PHYTOPHTHORA CINNAMOMI RESISTANT AVOCADO ROOTSTOCKS THROUGH SOMACLONAL VARIATION
1990 Summary of Avocado Research, pages 5-10 Avocado Research Advisory Committee University of California, Riverside GENERATION AND SELECTION OF PHYTOPHTHORA CINNAMOMI RESISTANT AVOCADO ROOTSTOCKS THROUGH
More informationRapid in vitro regeneration of date palm (Phoenix dactylifera L.) cv. Kheneizi using tender leaf explant
Emir. J. Food Agric. 2014. 26 (6): 539-544 doi: 10.9755/ejfa.v26i6.18051 http://www.ejfa.info/ Regular Article PLANT TISSUE CULTURE Rapid in vitro regeneration of date palm (Phoenix dactylifera L.) cv.
More informationIN VITRO BULBLET INDUCTION FROM BULB SCALES OF ENDANGERED ORNAMENTAL PLANT MUSCARI AZUREUM
Article DOI: 10.2478/v10133-010-0024-4 A&EB IN VITRO BULBLET INDUCTION FROM BULB SCALES OF ENDANGERED ORNAMENTAL PLANT MUSCARI AZUREUM S. Uranbey 1, A. Ipek 1, M. Caliskan 2, E. Dundar 3, S. Cocu 4, D.
More informationIn vitro propagation of Santalum album L.
J.Natn.Sci.Foundation Sri Lanka 2015 43 (3):265-272 DOI: http://dx.doi.org/10.4038/jnsfsr.v43i3.7954 RESEARCH ARTICLE In vitro propagation of Santalum album L. M.K.P. Peeris and W.T.P.S.K. Senarath * Department
More informationRegeneration of Plantlets Through PLB (Protocorm-Like Body) Formation in Phalaenopsis Join Angle X Sogo Musadian
204 J. Math. Fund. Sci., Vol. 48, No. 3, 2016, 204-212 Regeneration of Plantlets Through PLB (Protocorm-Like Body) Formation in Phalaenopsis Join Angle X Sogo Musadian Dini Meilasari & Iriawati School
More informationMICROPHOPAGATION OF DATE PALM (PHOENIX TISSUE CULTURE TECHNIQUE
Annals of Arid Zone 28 (1&2): 137-141, 1989 MICROPHOPAGATION OF DATE PALM (PHOENIX DACTYLIFERA L.) CV KHADRA WY USING TISSUE CULTURE TECHNIQUE N.L. KACKAR, K.R. SOLANKI AND S.P. JOSHI Central Arid Zone
More informationIn vitro propagation of muskmelon (Cucumis melo L.) from nodal segments, shoot tips and cotyledonary nodes
Rajshahi University journal of life & earth and agricultural sciences ISSN 2309-0960 Vol. 41: 71-77, 2013 In vitro propagation of muskmelon (Cucumis melo L.) from nodal segments, shoot tips and cotyledonary
More informationEFFICIENT PLANT REGENERATION FROM EMBRYOGENIC CELL SUSPENSION CULTURE OF TWO DEEPWATER RICE (ORYZA SATIVA L.) VARIETIES
The Chittagong Univ. J. B. Sci.,Vol. 5(1 &2):91-103, 2010. EFFICIENT PLANT REGENERATION FROM EMBRYOGENIC CELL SUSPENSION CULTURE OF TWO DEEPWATER RICE (ORYZA SATIVA L.) VARIETIES L. KHALEDA AND M. AL-FORKAN
More informationSomatic embryogenesis and plant regeneration from immature seeds of Magnolia obovata Thunberg
Plant Biotechnol Rep (2007) 1:237 242 DOI 10.1007/s11816-007-0037-0 ORIGINAL ARTICLE Somatic embryogenesis and plant regeneration from immature seeds of Magnolia obovata Thunberg Yong Wook Kim Æ So Young
More informationTISSUE CULTURE II. Organogenesis. PlSc 300 LAB Learn tissue culture techniques that promote organ formation.
76 TISSUE CULTURE II Organogenesis PlSc 300 LAB 11 REFERENCE: Text: 663 666; 706 712; 717 718. OBJECTIVES: 1. Learn tissue culture techniques that promote organ formation. 2. Practice making leaf and shoot
More informationGRADUATE UNIVERSITY OF SCIENCE AND TECHNOLGY VU THI HIEN
MINISTRY OF EDUCATION AND TRAINING VIETNAM ACADEMY OF SCIENCE AND TECHNOLOGY GRADUATE UNIVERSITY OF SCIENCE AND TECHNOLGY ----------------------------- VU THI HIEN REGENERATION AND MICROPROPAGATION OF
More informationSweta Mishra and Yuvraj K Vala
2018; 7(2): 91-96 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2018; 7(2): 91-96 Received: 07-01-2018 Accepted: 08-02-2018 Sweta Mishra Biotechnology Section, Central Instrumentation Laboratory, Sardarkrushinagar
More informationOrganogenic plant regeneration via callus induction in chickpea (Cicer arietinum L.) Role of genotypes, growth regulators and explants
Indian Journal of Biotechnology Vol. 4, April 2005, pp. 251-256 Organogenic plant regeneration via callus induction in chickpea (Cicer arietinum L.) Role of genotypes, growth regulators and explants Anju
More informationCHAPTER 2. IN VITRO REGENERATION OF Gerbera jamesonii Bolus Ex. Hook f. Previous research has proven that Gerbera jamesonii could successfully be
CHAPTER 2 IN VITRO REGENERATION OF Gerbera jamesonii Bolus Ex. Hook f. 2.1 EXPERIMENTAL AIMS Previous research has proven that Gerbera jamesonii could successfully be propagated in vitro. Different types
More informationDevelopment of a suitable plant regeneration protocol of cotton cultivar variety in comparison to non cultivar Coker
IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) ISSN: 2455-264X, Volume 2, Issue 5 (Jul. Aug. 2016), PP 14-20 Development of a suitable plant regeneration protocol of cotton cultivar variety
More informationINDIGENOUS PRODUCTION OF SYNTHETIC SEEDS IN DAUCUS CAROTA
Pak. J. Bot., 39(3): 849-855, 2007. INDIGENOUS PRODUCTION OF SYNTHETIC SEEDS IN DAUCUS CAROTA ZAKIA LATIF 1, IDREES, A. NASIR 2 AND S. RIAZUDDIN 3 1 Department of Mycology and Plant Pathology, 1 E-mail:
More informationStandardization of an in vitro regeneration protocol in gerbera (Gerbera jamesonii Bolus ex. Hooker F.)
Standardization of an in vitro regeneration protocol in gerbera (Gerbera jamesonii Bolus ex. Hooker F.) Koushik Dutta and Subhendu S. Gantait* Department of Floriculture, Medicinal and Aromatic Plants,
More informationRegeneration potential of seedling explants of chilli (Capsicum annuum)
African Journal of Biotechnology Vol. 8 (4), pp. 591-596, 18 February, 2009 Available online at http://www.academicjournals.org/ajb ISSN 1684 5315 2009 Academic Journals Full Length Research Paper Regeneration
More informationTHE EFFECT OF DIFFERENT PLANT GROWTH REGULATORS ON SHOOT INDUCTION OF Orthosiphon aristatus Boldingh.
THE EFFECT OF DIFFERENT PLANT GROWTH REGULATORS ON SHOOT INDUCTION OF Orthosiphon aristatus Boldingh. R. Zaidah & M.S. Nazri Biology Department, Faculty of Science, UTM, 81310 UTM Skudai, Johor, Malaysia.
More informationFaculty Member & Staff Data form C.V. PROFESSOR Doctor MAAZ AHMAD MOHAMAD ABDALLA ALGADA. Department of Architecture. Faculty of Engineering
C.V. PROFESSOR Doctor MAAZ AHMAD MOHAMAD ABDALLA ALGADA Department of Faculty of BANHA 2015 Name: MAAZ AHMAD A) PERSONAL DATA MOHAMAD ABDALLA ALGADA Date of Birth (Hijri) 7 / 12 / 1365 Date of Birth (Greg.)
More informationIn vitro regeneration system in brinjal (Solanum melongena L.) for stress tolerant somaclone selection
J. Bangladesh Agril. Univ. 7(2): 253 258, 2009 ISSN 1810-3030 In vitro regeneration system in brinjal (Solanum melongena L.) for stress tolerant somaclone selection A. Ferdausi, U. K. Nath, B. L. Das 1
More informationIN VITRO CALLUS INDUCTION AND PLANTLET REGENERATION IN FIG (FICUS CARICA L.)
Journal of Cell and Tissue Research Vol. 12(3) 3395-3400 (2012) (Available online at www.tcrjournals.com) ISSN: 0973-0028; E-ISSN: 0974-0910 Original Article IN VITRO CALLUS INDUCTION AND PLANTLET REGENERATION
More informationPlant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum orchids
Plant Cell, Tissue and Organ Culture 76: 11 15, 2004. 2003 Kluwer Academic Publishers. Printed in the Netherlands. 11 Plant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum
More informationDEVELOPMENT OF EFFICIENT CALLUS INITIATION OF MALTA (Citrus sinensis) THROUGH TISSUE CULTURE
ISSN: 2224-0616 Int. J. Agril. Res. Innov. & Tech. 1 (1&2): 64-68, December, 2011 Available at http://www.ijarit.webs.com DEVELOPMENT OF EFFICIENT CALLUS INITIATION OF MALTA (Citrus sinensis) THROUGH TISSUE
More informationShort Term Scientific Missions (STSM) Report. COST action FA1104: Sustainable production of high-quality cherries for the European market.
Short Term Scientific Missions (STSM) Report COST action FA1104: Sustainable production of high-quality cherries for the European market COST STSM Reference Number: COST-STSM-FA1104-25120 STMS Title: Planting,
More informationInternational Journal of ChemTech Research CODEN (USA): IJCRGG ISSN: Vol.8, No.11, pp , 2015
International Journal of ChemTech Research CODEN (USA): IJCRGG ISSN: 0974-4290 Vol.8, No.11, pp 113-119, 2015 Effect of growth on in vitro production of Hyoscyamus aureus L. and tropane alkaloids Khater
More information1JBoctor of ~bi[osopb!' in ~ortitultur
SOMACLONAL VARIATION IN BLACK PEPPER (~ifjt.\ n"j"'um l.) BY M.R.SHYLAJA THESIS Submitted in partial fulfilment of the requirement for the degree of 1JBoctor of ~bi[osopb!' in ~ortitultur Faculty of Agriculture
More informationIn vitro REGENERATION OF MUNGBEAN (Vigna radiata L.) FROM DIFFERENT EXPLANTS
Progress. Agric. 19(2) : 13-19, 2008 ISSN 1017-8139 In vitro REGENERATION OF MUNGBEAN (Vigna radiata L.) FROM DIFFERENT EXPLANTS M. K. Khatun, M. S. Haque, S. Islam and K. M. Nasiruddin Department of Biotechnology,
More informationGenetic and non genetic factors affecting callus induction and regeneration in sugarcane Sobhakumari V.P 1, Sreedivya M.J. 2 and Sanu Mary Abraham 3
Gregor Mendel Foundation Journal : -; 00. Genetic and non genetic factors affecting callus induction and regeneration in sugarcane Sobhakumari V.P, Sreedivya M.J. and Sanu Mary Abraham Tissue Culture Laboratory,
More informationSummary and conclusion
Summary and conclusion 6.1 Chemical sterilization of Explants Surface sterilization of explants collected from mature trees is necessary before initiation of any in vitro culture. In the present study
More informationInt.J.Curr.Microbiol.App.Sci (2017) 6(5):
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 5 (2017) pp. 1415-1422 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2017.605.154
More informationAgrobacterium-mediated Rice Transformation from Mature Seeds
Agrobacterium-mediated Rice Transformation from Mature Seeds Kimberly Nelson-Vasilchik, Joel Hague, and Albert Kausch The Plant Biotechnology Laboratory University of Rhode Island 530 Liberty Lane West
More informationIN VITRO REGENERATION OF PINEAPPLE (Ananas comosus) L. MERR 'MD2' USING DIFFERENT CONCENTRATION OF INDOLE ACETIC ACID (IAA) AND KINETIN (KN) HORMONE
IN VITRO REGENERATION OF PINEAPPLE (Ananas comosus) L. MERR 'MD2' USING DIFFERENT CONCENTRATION OF INDOLE ACETIC ACID (IAA) AND KINETIN (KN) HORMONE NORMA ZIATI BINTI ISMAIL Final Year Project Report Submitted
More informationStudies on Vegetative Propagation ~.f Tulips Regeneration of Bulblets in Bulb Scale Segments Culturt. 1 in vitro
J. Japan. Soc. Hort. Sci. 49(2) : 235-24u. ' QR0. Studies on Vegetative Propagation ~.f Tulips IV. Regeneration of Bulblets in Bulb Scale Segments Culturt. 1 in vitro Yoshlo NISHIUCHI Hokkaido University
More informationResearches regarding bitter melon (Momordica charantia) in vitro regeneration
Volume 14(3), 75-8, 21 JOURNAL of Horticulture, Forestry and Biotechnology Researches regarding bitter melon (Momordica charantia) in vitro regeneration Franţ Alexandra 1*, Botău Dorica 1 1 Banat s University
More informationPlant regeneration of Anthurium andreanum cv Rubrun
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol.7 No.3, Issue of December 15, 2004 2004 by Pontificia Universidad Católica de Valparaíso -- Chile Received June 4, 2004 / Accepted November 9, 2004
More informationWe observed a better response to the hormone treatments with the
GENETIC ENGINEERING OF I NONPAREIL I ALMOND Abhaya M. Dandekar l Thomas M. Gradziel l John Driver l Archie Tang l Sandra L. Uratsu. This project is aimed at developing genetic engineering technologies
More informationIdentity as an approach to civilization sustainability in the light of community partnership
Faculty of engineering Department of architecture Identity as an approach to civilization sustainability in the light of community partnership Research presented by En. Madiha Hamed Abd Elsattar Amasha
More informationIn vitro regeneration performance of Corchorus olitorius
J. Bangladesh Agril. Univ. 8(1): 1 6, 2010 ISSN 1810-3030 In vitro regeneration performance of Corchorus olitorius M. Hoque 1, K. M Nasiruddin 2, G. K. M. N. Haque 3 and G. C. Biswas 4 1 Dept. of Agronomy
More informationMicro propagation of sugarcane (Saccharum officinarum L.) through auxiliary buds
Micro propagation of sugarcane (Saccharum officinarum L.) through auxiliary buds P S \Varakagoda, S Subasinghe, D L C Kumari and T S Neththikumara Department of Crop Science, Faculty of Agriculture, University
More informationEfficient micropropagation of Vanilla planifolia Andr. under influence of thidiazuron, zeatin and coconut milk
Indian Journal of Biotechnology Vol 3, January 2004, pp. 113-118 Efficient micropropagation of Vanilla planifolia Andr. under influence of thidiazuron, zeatin and coconut milk P Giridhar and G A Ravishankar*
More informationPlant Tissue Culture Concepts and Laboratory Exercises
Plant Tissue Culture Concepts and Laboratory Exercises Edited by CRC Press Boca Raton New York London Tokyo Contents PART I. HISTORY OF PLANT TISSUE CULTURE Chapter one Topic History of plant tissue and
More informationUNIVERSITI TEKNOLOGI MARA THE CONDITION OF OUTDOOR SCULPTURES IN ASEAN SCULPTURE GARDEN KUALA LUMPUR: A CONSERVATION STUDY NORA SOFIA MOHAMED YURAN
UNIVERSITI TEKNOLOGI MARA THE CONDITION OF OUTDOOR SCULPTURES IN ASEAN SCULPTURE GARDEN KUALA LUMPUR: A CONSERVATION STUDY NORA SOFIA MOHAMED YURAN Thesis submitted in partial fulfilment ofthe requirements
More informationAppendix Ex vitro performance of peanut plants from TDZ-pretreated seeds
Appendix Ex vitro performance of peanut plants from TDZ-pretreated seeds INTRODUCTION Plant growth regulators (PGRs) are known to influence plant growth and development at very low concentrations (Jules
More informationMULTIPLE SHOOT REGENERATION IN DENDROBIUM FIMBRIATUM HOOK AN ORNAMENTAL ORCHID ABSTRACT
Kabir et al., The Journal of Animal & Plant Sciences, 23(4): 13, Page: J. 1140-1145 Anim. Plant Sci. 23(4):13 ISSN: 1018-7081 MULTIPLE SHOOT REGENERATION IN DENDROBIUM FIMBRIATUM HOOK AN ORNAMENTAL ORCHID
More informationCENTRE OF STUDIES FOR BUILDING SURVEYING FACULTY OF ARCHITECTURE, PLANNING AND SURVEYING UNIVERSITI TEKNOLOGI MARA
CENTRE OF STUDIES FOR BUILDING SURVEYING FACULTY OF ARCHITECTURE, PLANNING AND SURVEYING UNIVERSITI TEKNOLOGI MARA A STUDY ON AWARENESS AMONG PROFESSIONALS TOWARDS THE IMPORTANCE OF BUILDING SURVEYING
More informationUtilization of Embryogenic Cell Cultures for the Mass Production of Bulblets in Lilies
Utilization of Embryogenic Cell Cultures for the Mass Production of Bulblets in Lilies Sun Ki Kim and Byung Joon Ahn College of Bioresources Science Dankook University Cheonan 330-714 Korea Keywords: Oriental
More information