Plant Tissue Culture Concepts and Laboratory Exercises

Transcription

1 Plant Tissue Culture Concepts and Laboratory Exercises Edited by CRC Press Boca Raton New York London Tokyo

2 Contents PART I. HISTORY OF PLANT TISSUE CULTURE Chapter one Topic History of plant tissue and cell culture 3 James D. Caponetti, and PART II. SUPPORTING METHODOLOGIES Chapter two Topic General laboratory requirements, media and sterilization methods 11 Ellen G. Suiter Chapter three Laboratory Nutrition of callus cultures 27 James D. Caponetti Chapter four Topic Histological techniques 33, Kathleen R. Malueg and Effin T. Graham Chapter five Topic Photographic methods for plant cell and tissue culture 41 Chapter six Topic Statistical analysis of plant tissue culture data 47 Michael E. Compton PART III. PROPAGATION TECHNIQUES Chapter seven Topic Propagation from preexisting meristems 61 Chapter eight Laboratory Micropropagation of Syngonium by shoot culture 73

3 Chapter nine Laboratory Micropropagation of potato by node culture and microtuber production 81 Chapter ten Laboratory Micropropagation of lilacs 87 Deborah D. McCown and Andrew J. Daun Chapter eleven Topic Propagation from nonmeristematic tissues organogenesis 95 Otto J. Schwarz and Robert M. Beaty Chapter twelve Laboratory Direct shoot organogenesis from leaf explants of chrysanthemum 105 and Roger A. May Chapter thirteen Laboratory Shoot organogenesis from cotyledon explants of watermelon 113 Michael E. Compton and Chapter fourteen Laboratory Direct and indirect shoot organogenesis from leaves of Torenia fournieri 121 Mark P. Bridgen Chapter fifteen Laboratory Shoot organogenesis from petunia leaves 127 John E. Preece Chapter sixteen Topic Nonzygotic embryogenesis 133 Chapter seventeen Laboratory Embryogenic callus and suspension cultures from leaves of orchardgrass 149, and Bob V. Conger Chapter eighteen Laboratory Somatic embryogenesis from seeds of melon 161 Chapter nineteen Laboratory Somatic embryogenesis from mature peanut seed 167 Hazel Y. Wetzstein and Charleen Baker Chapter twenty Laboratory Direct somatic embryogenesis from leaves and flower receptacles of cineraria 175, Mary Catherine Scott and Kathleen R. Malueg Chapter twenty-one Laboratory Somatic embryogenesis from immature seeds of yellow poplar 183 Scott A. Merkle

4 Chapter twenty-two Laboratory Induction of somatic embryogenesis in conifers 191 Stephen M. Attree, Patricia J. Rennie and Larry C. Fowke PART IV. CROP IMPROVEMENT TECHNIQUES Chapter twenty-three Topic Use of protoplasts for plant improvement 201 Michael E. Compton, James A. Saunders and Richard E. Veilleux Chapter twenty-four Laboratory Isolation, culture and fusion of tobacco and potato protoplasts 215 Richard E. Veilleux and Michael E. Compton Chapter twenty-five Laboratory Isolation of protoplasts from leaves of chrysanthemum and orchardgrass 227 Chapter twenty-six Laboratory Isolation and culture of protoplasts from embryogenic yellow poplar suspension cultures 235 Scott A. Merkle Chapter twenty-seven Topic Haploid cultures 241 Sandra M. Reed Chapter twenty-eight Laboratory Production of haploid tobacco plants using anther culture 247 Sandra M. Reed \ Chapter twenty-nine Topic Transformation 253 Melina Lopez-Meyer, Ignacio E. Maldonado-Mendoza and Craig L. Nessler Chapter thirty Laboratory Transformation of tobacco and carrot using Agrobacterium tumefaciens and expression of the (3-glucuronidase (GUS) reporter gene 261 Ignacio E. Maldonado-Mendoza, Melina Lopez-Meyer and Craig L. Nessler Chapter thirty-one Laboratory t Transformation of chrysanthemum leaf explants using Agrobacterium tumefaciens 275 Chapter thirty-two Laboratory Construction and use of a simple gene gun for particle bombardment 283, Ernest Hiebert, Chia-Min Lin, Michael E. Compton and Victor P. Gaba Chapter thirty-three Topic Germplasm preservation 291 Leigh E. Towill

5 Chapter thirty-four Laboratory Vitrification as a method to cryopreserve shoot tips 297 Leigh E. Towill Chapter thirty-five Topic Secondary product expression in vitro 305 Mary Ann L. Smith Chapter thirty-six Laboratory Pigment production in Ajuga cell cultures 311 Mary Ann L. Smith Chapter thirty-seven Topic In vitro plant pathology 319 C. Jacyn Baker, Norton M. Mock and Elizabeth W. Orlandi PART V. SPECIAL APPLICATIONS Chapter thirty-eight Topic Commercial laboratory production 331 Gayle R. L. Suttle Chapter thirty-nine Topic Indexing for plant pathogens 341 Michael J. Klopmeyer PART VI. APPENDIX I. LIST OF SUPPLIERS 353 PART VII. INDEX 357