Preliminary results of propagation of several cherry rootstocks by green cuttings

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1 Preliminary results of propagation of several cherry rootstocks by green cuttings Daina Feldmane 1, Madalina Butac 2, Valentina Pole 1, Imants Missa 1 Latvia State Institute of Fruit-Growing 1, Research Institute for Fruit Growing Pitesti, Romania 2 1

2 Introduction Sour cherries (P. cerasus L.) have valuable traits as rootstocks for sweet cherry cultivars: more tolerant to wet soils, less vigorous trees, good fruit size, improved winterhardiness. The aim of the study was to find effective method for rooting and growing of green cuttings of sour cherry Latvijas Zemais and of cherry rootstock P7. 2

3 Materials and methods 3

4 Rootstocks: - Latvijas Zemais (Prunus cerasus L.), several clones - P7 (P. padus L. x (P. cerasus L. x P. avium L.)) Green cuttings were prepared in June and in June Dipped in the solution of rooting stimulators from to 8.00 o clock next morning (15 hours) - IBA 50 ml/ L (control) - Vitmin 10 ml /L - contains biologically active compounds (auxines, aminoacids, gibberellins, cytokinins) produced by some microorganisms (Pseudomonas spp., Azotobacter spp. etc.). - IBA 25 ml/l for P7 Planted - in peat substrate + perlite (control) - in peat substrate + perlite + Trihodermin (contains the cells of Trichoderma harizanum Rifai, stimulates growth, prevents diseases) 4

Grown in greenhouse with automatic air moistening

Ecklonia maxima) N: P: K = 3,6 : 8,2: 7,2 +

5 Grown in greenhouse with automatic air moistening and floor heating system Fertilized with - VITO Universal (control) N:P:K = 3,5 : 2,3 : 5 + microelements - Kelpak (extract of sea weeds Ecklonia maxima) N: P: K = 3,6 : 8,2: 7,2 + microelements + plant hormons Potted from 6th to 11th August (maxresdefault.jpg) 5

6 P7 LZ 51 Unheated floor Heated floor control IBA 50 ml/l IBA 25 ml/l (only P7) Vitmin 10 ml/l IBA 50 ml/l Peat substratum Peat substratum Peat substratum Peat substratum + Trihodermin Peat substratum VITO VITO VITO VITO Kelpak VITO 6

7 1 st 2 nd 3 rd During the potting - classified as 1st, 2nd,3rd categrory - counted, % calculated 7

8 Measurements at the end of vegetation season for the plants of 1st category: - the diameter of root neck, - shoot length, - shoot mass, - root mass. 8

9 RESULTS 9

10 Rooted cuttings of the clones of Latvijas Zemais (I) 100 rooted cuttings, % LZ Pure LZ 51 LZ 63 LZ 3 LZ 71 LZ Raunas st 2nd 3rd 10

11 Rooted cuttings of the clones of Latvijas Zemais (II) Latvijas Zemais 3, Latvijas Zemais 63, Latvijas Zemais Raunas positive effect if Trihodermin added to peat + perlite substratum rooted cuttings, % control Trihodermin 1st 2nd 3rd 11

12 Rooted cuttings of Latvijas Zemais rooted cuttings, % unheated control Kelpak Trihodermin Vitmin 1st 2nd 3rd 12

13 The growth of Latvijas Zemais 1st category plants root neck diameter, mm 4,0 3,5 3,0 2,5 2,0 1,5 1,0 0,5 0,0 2,7 3,1 3,2 control Kelpak Trihodermin shoot length, mm control Kelpak Trihodermin mass, g 7,0 6,0 5,0 4,0 3,0 2,0 1,0 0,0 5,0 2,7 6,0 1,8 1,7 2,7 control Kelpak root mass Trihodermin control Kelpak shoot mass Trihodermin 13

14 Rooted cuttings of P rooted cuttings, % unheated control Kelpak Trihoderm in Vitm in IBA 25 m l/l 1st 2nd 3rd 14

15 The growth of P7 1st category plants root neck diameter, mm 6,0 5,0 4,0 3,0 2,0 1,0 0,0 control 3,5 3,0 3,3 Kelpak Trihodermin IBA 25 ml/l 4, control 287 Kelpak Trihodermin 364 5,0 4,0 3,0 2,0 1,0 0,0 2,0 2,0 1,7 4,3 2,7 2,7 3,0 4,3 control Kelpak Trihodermin IBA 25 ml/l control Kelpak Trihodermin IBA 25 ml/l shoot length, mm IBA 25 ml/l mass, g root mass shoot mass 15

16 Conclusions Floor heating in greenhouse increased output of rooted cuttings and of 1st category plants. Rooting stimulator Vitmin caused weaker root and shoot growth for rootstocks but advanced callus formation for Latvijas Zemais if compared to using of IBA 50 ml/l. Dipping of cuttings in solution of IBA 25 ml/l improved the rooting, and lastingly enhanced shoot and root growth for rootstock P7 if compared to using of IBA 50 ml/l. Enhanced rooting and growth was observed for several clones of Latvijas Zemais if Trihodermin was added to peat substratum. No significant differences were observed for rootstock growth depending on fertilizing with Kelpak or VITO Universal. 16

of stone fruit trees propagation, quality improvement of generative

17 Acknowledgements This research is supported by European Social Fund project "Creation of a researcher group to investigate the possibilities of stone fruit trees propagation, quality improvement of generative processes and fruit usage. Project Nr. 2013/0048/1DP/ /13/APIA/VIAA/008 17

18 Thank you for attention! 18

Establishing new trees possible impacts of rootstock propagation method on young tree growth Ute Albrecht

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