for root traits selection

Transcription

1 Hishtil R.M Ltd HTS (High Throughput System) for root traits selection 1

2 The challenge Improve plant performance by direct root breeding. (The hidden-part of the plant) 2

3 Low soil temperature causes leaf dehydration and growth delay 3

4 Improvement of root performance, is hindered by the absence of efficient selection system. Tolerant wild Tomato Sensitive Commercial tomato4

5 The solution HTS by Hishtil R.M Practical selection method, without plant destruction High correlation with field results. 5

6 HTS advantages Controlled conditions. Large scale screening (10,000 s s of plants) in few weeks. Work all year round, no weather limitations. 6

7 HTS in seedling stage 7

8 HTS applications Identify cold tolerant lines. Prove potential heritability. Selection procedure for grafted plants. High correlation between HTS and field. 8

9 Tomato screening Source Populations Tolerant % Known hyb Selected lines

10 Tomato reactions to 5 o c T (Tolerant) Tolerant line MT (Medium Tolerant) Beaufort S (Sensitive) Shirley 10

11 Trait heritability (field) M 82 Sensitive T Tolerant F1 X T Tolerant 11

12 Cold Soil Tolerant Processing Tomato (2009 Trial) 12

13 Acre field test, Israel (Spring 2009) The seedlings where produced and grafted in Hishtil nursery Planting 9/2/2009 = 50 days prior to standard planting season Field Trial: Included 37 different rootstocks The rootstocks were grafted : - Non grafted - On themselves -With as scion - With Heinz 9780 as scion Notes: Lines HRM17 and HRM19 are cold tolerant genetic material under development by Hishtil RM. 13

14 Tomato HTS at 5 o c Example: 80 X HRM 19 M S T T Beaufort Shirley Tolerant line 80 X HRM19 14

15 T (80 X HRM19) S (23/4/2009) (19/3/2009) 15

16 T (80 X HRM19) S (21/5/2009) Fruits weight per plant (g) Rootstock Very small Small Medium Large Red Total 80xHRM , , ,922.0 Beaufort , , ,

17 T (80 X HRM19) S (10/6/2009) Fruits weight per plant (g) Rootstock Small Medium Large Red Total 80xHRM , , , ,805.3 Beaufort , , , , , , ,

18 T (80 X HRM19) S (21/5/2009) (10/6/2009) 18

19 Fruit weight per plant (80 X HRM19) T XHRM xHRM Beaufort 3 Beaufort Red Large Medium Small Very small Red Large Medium Small 19

20 Heinz 9780 (80 X HRM19) T Heinz 9780 Heinz 9780 S (21/5/2009) Fruits weight per plant (g) Rootstock Very small Small Medium Large Total 80xHRM , , , ,436.7 Beaufort ,860.0 Heinz ,

21 Heinz 9780 T (80 X HRM19) Heinz 9780 Heinz 9780 S (18/6/2009) Fruits weight per plant (g) Rootstock Small Medium Large Red Total 80XHRM , , ,250.7 Beaufort Heinz , , , , , ,

22 Heinz 9780 T (80 X HRM19) (21/5/2009) Heinz 9780 Heinz 9780 S (18/6/2009) 22

23 Fruit weight per plant Heinz9780 (80 X HRM19) T 8 80XHRM Beaufort Heinz Red Large Medium Small 23

24 Tomato HTS at 5 o c : Example HRM19 X HRM 17 BeaufortM Shirley S Tolerant line T HRM19 XHRM17 T 24

25 T (HRM19 X HRM17) S (23/4/2009) (19/3/2009) 25

26 (HRM19 X HRM17) S (21/5/2009) 26

27 T (HRM19 X HRM17) S (10/6/2009) Fruits weight per plant (g) Rootstock Small Medium Large Red Total HRM19XHRM , , , ,483.3 Beaufort , , , , , , ,

28 T (HRM19 X HRM17) S (21/5/2009) (10/6/2009) 28

29 Fruit weight per plant (HRM19 X HRM17) T HRM19XHRM Beaufort HRM19XHRM Red Large Medium Small Very small 2 Beaufort 1 0 Red Large Medium Small 29

30 Heinz 9780 T (HRM19 X HRM17) Heinz 9780 Heinz 9780 S (23/4/2009) (19/3/2009) 30

31 Heinz 9780 T Heinz 9780 S (HRM19 X HRM17) Heinz 9780 (18/6/2009) Fruits weight per plant (g) Rootstock Small Medium Large Red Total HRM19XHRM , , ,882.7 Beaufort Heinz , , , , , ,

32 Fruit weight per plant HRM19XHRM Beaufort Heinz Red Large Medium Small 32

33 Different rootstocks fruit weight as scion 33

34 Fruit weight per plant XHRM19 HRM19XHRM XHRM17 3 ShXHRM19 Beaufort 2 1 Heinz Red Large Medium Small 34

35 Different rootstocks fruit weight Heinz9780 as scion 35

36 Fruit weight per plant XHRM XHRM17 HRM19XHRM Beaufort Heinz Red Large Medium Small 36

37 Tolerant versus Sensitive Plants Selection at 5 o C Control 158 Commercial Rootstock T Tolerant T Tolerant 37

38 Tolerant versus Sensitive Plants (In the Field) Tolerant Hybrid T Sensitive Line S Tolerant Hybrid T 38

39 Different Rootstocks with same commercial scion Commercial line Commercial line Commercial line Sensitive Line Commercial line Tolerant Hybrid Control Sensitive Tolerant 39

40 כנות scion שונות עם רוכב ז commercial הה (זן with מסחרי) שתיל Rootstocks ת ספטמבר Different (September planting) Commercial line Sensitive Line Commercial line Tolerant Hybrid 40

41 Different Rootstocks with commercial scion (September planting) Commercial line Commercial line Commercial line Sensitive Line Commercial line Tolerant Hybrid 80 C m 130 C m 41

42 Summary Tolerant genetic material was identified and is being developed. The selected lines were used as rootstock to demonstrate the contribution of the root trait to the ability of the plant to grow under low soil temperature The rootstock s contribution is dominating The trait is heritable High Throughput Selection results correlate with field outcomes The system is applicable to multiple crops 42

43 Hishtil R.M Ltd Thank you 43