CEN/TC 275. Secretariat of CEN/TC 275. Resolutions of the 16 th meeting of CEN/TC 275 in Berlin on 3 rd and 4 th July 2006

Transcription

1 CEN/TC 275 N 0360 "Food analysis Horizontal methods" Secretariat of CEN/TC 275 Carola Seiler DIN Deutsches Institut für Normung e.v. D Berlin International International carola.seiler@din.de CEN/TC 275 Resolutions of the 16 th meeting of CEN/TC 275 in Berlin on 3 rd and 4 th July 2006 For information. Some editorial corrections (writing errors) have been made on the draft resolutions. The resolutions as dispatched herewith have been adopted by members of CEN/TC 275 by correspondence after the meeting. See also doc CEN/TC 275 N 0359

2 - CEN/TC 275 N 360 page 1 - Resolutions of the 16 th meeting of CEN/TC 275 in Berlin on 3 rd and 4 th July 2006 Resolution 01/2006/00 taken by CEN/TC 275 on Subject: CEN/TC 275 Re-appointment of the Chairperson The CEN/TC 275 Food analysis Horizontal methods, - considering the CEN/CENELEC Internal Regulations - Part 2, subclause 3.2.2, which lays down the rules for nomination, appointment and responsibilities of Chairpersons; - noting the nomination by the Technical Committee Secretariat; - noting the commitment of the applicant to the responsibilities and duties of a Technical Committee Chairperson as given in the CEN BOSS; decides to re-appoint Prof. Dr. Paulus, as Chairman of CEN/TC 275 for a period of three years starting on , noting that Prof. Dr. Klaus Paulus is Chairperson of the Technical Committee since The decision was taken : Resolution 02/2006/00 taken by CEN/TC 275 on Subject: CEN/TC 275 Confirmation of existing ENs 1376, 1377, 1378, 1379, , , , , 1784, EN 1786, 1788, 12822, , 12856, 12857, 13585, 13708, 13783, 13784, of ENVs and and of CEN/TS CEN/TC 275 "Food analysis horizontal methods" - having considered the CEN/CENELEC Internal Regulations - Part 2, subclause stating the obligation of the review of ENs at intervals not exceeding five years; - having noted the resolution CEN/BT 5/1995, fixing the procedure to be followed when reviewing; decides to confirm EN 1376:1996 "Foodstuffs - Determination of saccharin in table top sweetener preparations - Spectrometric method" EN 1377:1996 "Foodstuffs - Determination of acesulfame K in table top sweetener preparations - Spectrometric method" EN 1378:1996 "Foodstuffs - Determination of aspartame in table top sweetener preparations - Method by high performance liquid chromatography" EN 1379:1996 "Foodstuffs - Determination of cyclamate and saccharin in liquid table top sweetener preparations - Method by high performance liquid chromatography" EN :1996 "Fatty food - Determination of pesticides and polychlorinated biphenyls (PCBs) - Part 1: General" EN :1996 "Fatty food - Determination of pesticides and polychlorinated biphenyls (PCBs) - Part 2: Extraction of fat, pesticides and PCBs, and determination of fat content" EN :1996 "Fatty food - Determination of pesticides and polychlorinated biphenyls (PCBs) - Part 3: Clean-up methods" EN :1996 "Fatty food - Determination of pesticides and polychlorinated biphenyls (PCBs) - Determination, confirmatory tests, miscellaneous" EN 1784:2003 "Foodstuffs - Detection of irradiated food containing fat - Gas chromatographic analysis of hydrocarbons" EN 1786:1996 "Foodstuffs - Detection of irradiated food containing bone - Method by ESR spectroscopy" EN 1788:2001 "Foodstuffs - Thermoluminescence detection of irradiated food from which silicate minerals can be isolated" EN 12822:2000 "Foodstuffs - Determination of vitamin E by high performance liquid chromatography -

3 - CEN/TC 275 N 360 page 2 - Measurement of alpha-, beta-, gamma-, and delta-tocopherols" EN :2000 "Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 2: Measurements of Beta-carotene" EN 12856:1999 "Foodstuffs - Determination of acesulfame-k, aspartame and saccharin - High performance liquid chromatographic method" EN 12857:1999 "Foodstuffs - Determination of cyclamate - High performance liquid chromatographic method" EN 13585:2001 "Foodstuffs - Determination of fumonisins B1 and B2 in maize - HPLC method with solid phase extraction clean-up" EN 13708:2001 "Foodstuffs - Detection of irradiated food containing crystalline sugar by ESR spectroscopy" EN 13783:2001 "Foodstuffs - Detection of irradiated food using Direct Epifluorescent Filter Technique/Aerobic Plate Count (DEFT/APC) - Screening method" EN 13784:2001 "Foodstuffs - DNA Comet Assay for the detection of irradiated foodstuffs - Screening method" CEN/TC 275 "Food analysis horizontal methods" - considering the result and the comments made during the review of ENV 14164:2002 "Foodstuffs - Determination of vitamin B 6 by HPLC" ENV 14166:2001 "Foodstuffs - Determination of vitamin B 6 by microbiological assay" CEN/TS 14537:2003 "Foodstuffs - Determination of neohesperidin-dihydrochalcon" decides to extend the life of the ENV and CEN/TS for another 2 years and confirms the life of the ENV and CEN/TS has never been extended before. The decision on all ENs, ENVs and CEN/TS was taken. Resolution 03/2006/00 taken by CEN/TC 275 on Subject: CEN/TC Activation of a preliminary work item CEN/TC 275 "Food analysis horizontal methods" having considered the proposal for a new work item as documented in CEN/TC 275 N 343 confirming that the new work item falls within its scope; confirming that the new work item corresponds to real market needs; confirming that the resources to complete the work as described below are available; decides to register the work item described below in its active programme of work. * 1 This item corresponds to the activation of work item currently registered at preliminary stage * 2 This adoption is based on the availability of a (pre) first draft CEN/TC 275 N 343 * 3 The decision was taken * 4 Title Foodstuffs Determination of acesulfame-k, aspartame, neohesperidinedihydrochalcone and saccharin High performance liquid chromatographic method

4 - CEN/TC 275 N 360 page 3 - * 5 Scope This European Standard specifies a high performance liquid chromatographic (HPLC) method for the determination of acesulfame-k, aspartame, neohesperidine dihydrochalcole and saccharin in foodstuffs. The method has been fully validated through collaborative trial, to internationally recognised standards, on the following analyte:matrix combinations: acesulfame-k and aspartame in water-based drink, fruit-based drink, cheesecake with biscuit base, canned soup and instant chocolate drink; saccharin in water-based drink, fruit-based drink, cheesecake with biscuit base and canned soup; and neohesperidine dihydrochalcone in water-based drink, fruit-based drink and canned soup. * 6 Deliverable EN TS TR * 7 Track Enquiry + Formal Vote (ENQ + FV) 8 Reference Document (if any) CEN/TC 275 N 343 * 9 Document developed in WG 2 Sweeteners * 10 Intended to be mandated not known * 11 Related Directive(s) Yes; European Parliament and Council Directive 94/35/EC of 30 June 1994 on sweeteners for use in foodstuffs amended by directive 2003/115 For citation in Official Journal? not known * 12 Commitment: The following CEN members, AFNOR, BSI, DIN, DS, NEN are committed to participate actively in the development of the project. 13 Vienna Agreement not applicable * 14 Timeframe months max. (circulation of first working draft) months max. (dispatch of draft for PQ/ENQ/UAP or TCA to CMC) months max. (dispatch of draft for FV to CMC) Note: Stage code only valid for EN deliverable The project leader is Dr. Roger Wood. CEN/TC 275 accepts doc CEN/TC 275 N 343 as first working document and agrees to launch the CEN-Enquiry on this project after final editorial corrections made by the secretariats of WG 2 and CEN/TC 275. Resolution 04/2006/00 taken by CEN/TC 275 on Subject: CEN/TC 275 Specification of the names of WG 3 and WG 4 CEN/TC 275 "Food analysis Horizontal methods" adopts the proposal made by CEN/TC 275/WG 4 to change its title from "Pesticides in non-fatty foods" into "Pesticides in foods of plant origin". Proposes that the name of WG 3 shall be changed from Pesticides and PCBs in fatty foods" into Pesticides and PCBs in foods of animal origin under the condition that WG 3 accepts this proposal by written procedure. The first part of this resolution is applicable from 4 th July. The second part of this resolution is applicable from the moment on when WG 3 has agreed this proposal by written procedure.

5 - CEN/TC 275 N 360 page 4 - Resolution 05/2006/00 taken by CEN/TC 275 on Subject: CEN/TC 275 Appointment of the Convenor of Working Groups 3 and 4 CEN/TC 275 "Food analysis Horizontal methods" - considering the CEN/CENELEC Internal Regulations - Part 2, clause 3.4.2, which lays down the rules for the appointment and responsibilities of a Working Group Convenor; - noting the nomination as evaluated by the Technical Committee Secretariat; - noting the commitment of the applicant to his responsibilities and duties; - noting the commitment of the professional standardization support from DIN; decides to appoint Dr. Lutz Alder as Convenor of Working Group 3 "Pesticides in food of animal origin" and Working Group 4 "Pesticides in food of plant origin" until the completion of the tasks assigned to the Working Groups. The decision was taken. Resolution 06/2006/00 taken by CEN/TC 275 on Subject: CEN/TC 275 Adoption of a new work item CEN/TC 275 "Food analysis horizontal methods" having considered the proposal for a new work item confirming that the new work item falls within its scope; confirming that the new work item corresponds to real market needs; confirming that the resources to complete the work as described below are available; decides to register the work item described below in its active programme of work. * 1 This item corresponds to the revision of EN :1998 * 2 This adoption is based on other justification: Title and references shall be updated and be aligned to the new versions of part 2 and part 3, see resolution no 07/2006/00 * 3 The decision was taken * 4 Title Foods of plant origin Multiresidue methods for the gas chromatographic determination of pesticide residues - Part 1: General considerations * 5 Scope This European Standard gives general considerations for the determination of pesticide residues in non-fatty foods. Each method described in this European Standard is suitable for identifying and quantifying a definite range of those organohalogen, and/or organophosphorus and/or organonitrogen pesticides which occur as residues in foodstuffs of plant origin. This European Standard contains the following methods that have been subjected to interlaboratory studies and/or are adopted throughout Europe: - method L: Extraction with acetone, liquid-liquid partition with dichloromethane and clean-up on a silica-gel/charcoal column [1]; - method M: Extraction with acetone and liquid-liquid partition with

6 - CEN/TC 275 N 360 page 5 - dichloromethane/light petroleum, if necessary clean-up on Florisil [2, 3, 4]; - method N: Extraction with acetone, liquid-liquid partition with dichloromethane and clean-up with gel permeation and silica gel chromatography [5]; - method O: Extraction with acetonitrile, liquid-liquid partition with light petroleum and clean-up on a Florisil column [6]; - method P: Extraction of organophosphorus compounds with ethyl acetate and, if necessary, clean-up with gel permeation chromatography [7]. The applicability of the five methods L to P for residue analysis of organohalogen, organophosphorus and organonitrogen pesticides, respectively, is given for each method. * 6 Deliverable EN TS TR * 7 Track Enquiry + Formal Vote (ENQ + FV) 8 Reference Document (if any) EN * 9 Document developed in WG 4 "Pesticides in foods of plant origin" * 10 Intended to be mandated not known * 11 Related Directive(s) Yes; 396/2005 For citation in Official Journal? not known * 12 Commitment: The following CEN members, AFNOR, BSI, DIN, DS, NEN are committed to participate actively in the development of the project. 13 Vienna Agreement not applicable * 14 Timeframe months max. (circulation of first working draft) months max. (dispatch of draft for PQ/ENQ/UAP or TCA to CMC) months max. (dispatch of draft for FV to CMC) Note: Stage code only valid for EN deliverable The project leader is Dr. Birgit Polonji. CEN/TC 275 notices that WG 4 will elaborate a draft to be submitted to the CEN-Enquiry at its next meeting in October This draft shall be considered by the secretariat of CEN/TC 275 and convener and does not need a resolution by correspondence, according to 3.3 and 3.4 of the chapter "Drafting of European Standards up to enquiry stage" in CEN-BOSS, Production process. Resolution 07/2006/00 taken by CEN/TC 275 on Subject: CEN/TC 275 Adoption of a new work item CEN/TC 275 "Food analysis horizontal methods" having considered the proposal for a new work item as documented in CEN/TC 275 N 273; confirming that the new work item falls within its scope; confirming that the new work item corresponds to real market needs; confirming that the resources to complete the work as described below are available; decides to register the work item described below in its active programme of work.

7 - CEN/TC 275 N 360 page 6 - * 1 This item corresponds to the revision of EN :1998 * 2 This adoption is based on the availability of a (pre) first draft CEN/TC 275 N 273 * 3 The decision was taken * 4 Title Foods of plant origin Multiresidue methods for the gas chromatographic determination of pesticide residues - Part 2: Methods for extraction and clean-up * 5 Scope This European Standard specifies methods for the extraction and clean-up of non fatty food samples for quantitative determination of pesticide residues. Different solvents can be used for this purpose. These pesticide residues are generally associated with other co-extracted compounds which would interfere in the analysis. To purify the crude extracts to be analysed, several techniques can be used. This standard contains the following extraction and clean-up methods that have been subjected to interlaboratory studies and /or are adopted throughout Europe: - method L: Extraction with acetone, liquid liquid partition with dichloromethane and clean up on a silica gel/charcoal column [1]; - method M: Extraction with acetone and liquid liquid partition with dichloromethane/light petroleum, if necessary clean-up on Florisil 1) [2, 3, 4]; - method N: Extraction with acetone, liquid liquid partition with dichloromethane or cyclohexane/ethyl acetate and clean up with gel permeation and silica gel chromatography [5, 8]; - method O: Extraction with acetonitrile, liquid liquid partition with light petroleum and clean up on a Florisil column [6]; - method P: Extraction with ethyl acetate, and if necessary, clean up by gel permeation chromatography [7]. This European Standard specifies the details of methods L to P for the extraction and the clean-up of samples of non-fatty food. Several solvents at different volumes are used for extraction. Techniques of clean-up are listed such as liquidliquid partition, liquid chromatography on various adsorbents and gel permeation chromatography. A table providing the couples (matrix/pesticide) which have been submitted to collaborative studies and a list of indicative applicability of the method to different pesticides are given for each method, wherever possible. * 6 Deliverable EN TS TR * 7 Track Enquiry + Formal Vote (ENQ + FV) 8 Reference Document (if any) CEN/TC 275 N 273 * 9 Document developed in WG 4 "Pesticides in foods of plant origin" * 10 Intended to be mandated not known * 11 Related Directive(s) Yes; 396/2005 For citation in Official Journal? not known * 12 Commitment: The following CEN members, AFNOR, BSI, DIN, DS, NEN, are committed to participate actively in the development of the project. 13 Vienna Agreement not applicable

8 - CEN/TC 275 N 360 page 7 - * 14 Timeframe months max. (circulation of first working draft) months max. (dispatch of draft for PQ/ENQ/UAP or TCA to CMC) months max. (dispatch of draft for FV to CMC) Note: Stage code only valid for EN deliverable The project leader is Dr. Birgit Polonji. CEN/TC 275 accepts doc CEN/TC 275 N 343 as first working document. CEN/TC 275 notices that WG 4 will elaborate a draft to be submitted to the CEN-Enquiry at its next meeting in October This draft shall be considered by the secretariat of CEN/TC 275 and convener and does not need a resolution by correspondence, according to 3.3 and 3.4 of the chapter "Drafting of European Standards up to enquiry stage" in CEN-BOSS, Production process. Resolution 08/2006/00 taken by CEN/TC 275 on Subject: CEN/TC 275 Adoption of a new work item CEN/TC 275 "Food analysis horizontal methods" having considered the proposal for a new work item confirming that the new work item falls within its scope; confirming that the new work item corresponds to real market needs; confirming that the resources to complete the work as described below are available; decides to register the work item described below in its active programme of work. * 1 This item corresponds to the revision of EN :1998 * 2 This adoption is based on other justification: Title and references shall be updated and be aligned to the new versions of part 1 and part 2, see resolution no 07/2006/00 * 3 The decision was taken * 4 Title Foods of plant origin Multiresidue methods for the gas chromatographic determination of pesticide residues - Part 3: Determination and confirmatory tests * 5 Scope This European Standard gives guidance on some recommended techniques for the determination of pesticide residues in non-fatty foods and on confirmatory tests. The identity of any observed pesticide residue is confirmed, particularly in those cases in which it would appear that the maximum residue limit has been exceeded. * 6 Deliverable EN TS TR * 7 Track Enquiry + Formal Vote (ENQ + FV) 8 Reference Document (if any) EN * 9 Document developed in WG 4 "Pesticides in foods of plant origin" * 10 Intended to be mandated not known

9 - CEN/TC 275 N 360 page 8 - * 11 Related Directive(s) Yes; 396/2005 For citation in Official Journal? not known * 12 Commitment: The following CEN members, AFNOR, BSI, DIN, DS, NEN, are committed to participate actively in the development of the project. 13 Vienna Agreement not applicable * 14 Timeframe months max. (circulation of first working draft) months max. (dispatch of draft for PQ/ENQ/UAP or TCA to CMC) months max. (dispatch of draft for FV to CMC) Note: Stage code only valid for EN deliverable The project leader is Dr. Birgit Polonji. CEN/TC 275 notices that WG 4 will elaborate a draft to be submitted to the CEN-Enquiry at its next meeting in October This draft shall be considered by the secretariat of CEN/TC 275 and convener and does not need a resolution by correspondence, according to 3.3 and 3.4 of the chapter "Drafting of European Standards up to enquiry stage" in CEN-BOSS, Production process. Resolution 09/2006/00 taken by CEN/TC 275 on Subject: CEN/TC 275 Adoption of a new work item CEN/TC 275 "Food analysis horizontal methods" having considered the proposal for a new work item as documented in CEN/TC 275 N 274; confirming that the new work item falls within its scope; confirming that the new work item corresponds to real market needs; confirming that the resources to complete the work as described below are available; decides to register the work item described below in its active programme of work. * 1 This item corresponds to * 2 This adoption is based on the availability of a (pre) first draft CEN/TC 275 N 274 * 3 The decision was taken * 4 Title Foods of plant origin Determination of pesticide residues using -MS and/or LC- MS(/MS) folowing acetonitrile extraction/partitioning - QuEChERS method * 5 Scope This European Standard describes a method for the analysis of pesticide residues in produce with a low fat content, such as fruits, vegetables, cereals as well as processed products thereof including dried fruit. The method has been collaboratively studied on cucumber, lemon, wheat flour, raisins, apples, oranges and lettuce.

10 - CEN/TC 275 N 360 page 9 - * 6 Deliverable EN TS TR * 7 Track Enquiry + Formal Vote (ENQ + FV) 8 Reference Document (if any) CEN/TC 275 N 274 * 9 Document developed in WG 4 "Pesticides in foods of plant origin" * 10 Intended to be mandated not known * 11 Related Directive(s) Yes; 396/2005 For citation in Official Journal? not known * 12 Commitment: The following CEN members, AFNOR, BSI, DIN, DS, NEN, are committed to participate actively in the development of the project. 13 Vienna Agreement not applicable * 14 Timeframe months max. (circulation of first working draft) months max. (dispatch of draft for PQ/ENQ/UAP or TCA to CMC) months max. (dispatch of draft for FV to CMC) Note: Stage code only valid for EN deliverable The project leader is Dr. Michelangelo Anastassiades. CEN/TC 275 notices that WG 4 will elaborate a draft to be submitted to the CEN-Enquiry at its next meeting in October This draft shall be considered by the secretariat of CEN/TC 275 and convener and does not need a resolution by correspondence, according to 3.3 and 3.4 of the chapter "Drafting of European Standards up to enquiry stage" in CEN-BOSS, Production process. Resolution 10/2006/00 taken by CEN/TC 275 on Subject: CEN/TC 275 Adoption of a new work item CEN/TC 275 "Food analysis horizontal methods" having considered the proposal for a new work item as documented in CEN/TC 275 N 275; confirming that the new work item falls within its scope; confirming that the new work item corresponds to real market needs; confirming that the resources to complete the work as described below are available; decides to register the work item described below in its active programme of work. * 1 This item corresponds to * 2 This adoption is based on the availability of a (pre) first draft CEN/TC 275 N 275 * 3 The decision was taken

11 - CEN/TC 275 N 360 page 10 - * 4 Title Foods of plant origin Determination of pesticide residues using LC-MS/MS following methanol extraction and clean-up using diatomaceous earth * 5 Scope This draft European Standard specifies a method using high performance liquid chromatography/tandem mass spectrometry (LC-MS/MS) for the simultaneous determination of a large number of pesticide residues in crops. * 6 Deliverable EN TS TR * 7 Track Enquiry + Formal Vote (ENQ + FV) 8 Reference Document (if any) CEN/TC 275 N 275 * 9 Document developed in WG 4 "Pesticides in foods of plant origin" * 10 Intended to be mandated No not known * 11 Related Directive(s) Yes; 396/2005 For citation in Official Journal? not known * 12 Commitment: The following CEN members, AFNOR, BSI, DIN, DS, NEN, are committed to participate actively in the development of the project. 13 Vienna Agreement not applicable * 14 Timeframe months max. (circulation of first working draft) months max. (dispatch of draft for PQ/ENQ/UAP or TCA to CMC) months max. (dispatch of draft for FV to CMC) Note: Stage code only valid for EN deliverable The project leader is Dr. Lutz Alder. CEN/TC 275 notices that WG 4 will elaborate a draft to be submitted to the CEN-Enquiry at its next meeting in October This draft shall be considered by the secretariat of CEN/TC 275 and convener and does not need a resolution by correspondence, according to 3.3 and 3.4 of the chapter "Drafting of European Standards up to enquiry stage" in CEN-BOSS, Production process. Resolution 11/2006/00 taken by CEN/TC 275 on Subject: CEN/TC 275 Adoption of a new work item CEN/TC 275 "Food analysis horizontal methods" having considered the proposal for a new work item as documented in CEN/TC 275 N 336; confirming that the new work item falls within its scope; confirming that the new work item corresponds to real market needs; confirming that the resources to complete the work as described below are available; decides to register the work item described below in its active programme of work. * 1 This item corresponds to * 2 This adoption is based on the availability of a (pre) first draft CEN/TC 275 N 336

12 - CEN/TC 275 N 360 page 11 - * 3 The decision was taken * 4 Title Food analysis - Determination of pesticide residues by LC-MS/MS Collection of mass spectrometric parameters * 5 Scope This Technical Report lists mass spectrometric parameters which are useful for the application of European standards for the determination of pesticide residues in foods of plant origin that use LC-MS/MS, such as the standards in preparation: EN xxx "Foods of plant origin Determination of pesticide residues using LC- MS/MS following methanol extraction and clean up using diatomaceous earth" EN xxx "Foods of plant origin Determination of pesticide residues using GC-MS and/or LC-MS/MS following acetonitrile extraction/partitioning QuEChERSmethod * 6 Deliverable EN TS TR * 7 Track Enquiry + Formal Vote (ENQ + FV) TC Approval (TCA) by correspondence 8 Reference Document (if any) CEN/TC 275 N 336 * 9 Document developed in WG 4 "Pesticides in foods of plant origin" * 10 Intended to be mandated not known TC Approval (TCA) in meeting * 11 Related Directive(s) Yes; 396/2005 For citation in Official Journal? not known * 12 Commitment: The following CEN members, Denmark, France, Germany, The Netherlands, UK, are committed to participate actively in the development of the project. 13 Vienna Agreement not applicable * 14 Timeframe months max. (circulation of first working draft) months max. (dispatch of draft for PQ/ENQ/UAP or TCA to CMC) months max. (dispatch of draft for FV to CMC) Note: Stage code only valid for EN deliverable The project leader is Dr. Magnus Jezussek CEN/TC 275 notices that WG 4 will elaborate a draft to be submitted to the CEN-Formal Vote at its next meeting in October This draft shall be considered by the secretariat of CEN/TC 275 and convener and does not need a resolution by correspondence, according to 3.3 and 3.4 of the chapter "Drafting of European Standards up to enquiry stage" in CEN-BOSS, Production process. Resolution 12/2006/01 taken by CEN/TC 275 on CEN/TC 275 "Food analysis horizontal methods" having considered the proposal for a new work item as documented in CEN/TC 275 N 331;

13 - CEN/TC 275 N 360 page 12 - * C Title Foodstuffs Determination of patulin in fruit juice and fruit based puree - HPLC method with liquid/liquid partition clean-up and solid phase extraction * D Scope This European Standard specifies a method for the determination of patulin in fruit juices and fruit based purees such as baby food purees using high performance liquid chromatography (HPLC). The method has been validated for the determination of patulin via the analysis of naturally contaminated and spiked samples in apple juice at levels ranging from 3,0 µg/kg up to 15,5 µg/kg and in baby food based fruit purees at levels ranging from 3,4 µg/kg up to 17,9 µg/kg. Baby food fruit purees used in this study contained mainly the following ingrediences: blueberry, apple, banana, lemon, wheat biscuits, wheat syrup, whole milk and vegetable oils as ingredience as commercially available products on the European market. * E Deliverable EN TS TR * F Track Enquiry + Formal Vote (ENQ + FV) G Document developed in WG 5 "Biotoxins", Project leader: Joerg Stroka H Intended to be mandated No Yes; M/383/EN, item 1 I Related Directive(s) No Yes; 315/1993 or 466/2001 For citation in Official Journal? not known The following CEN members AFNOR, BSI, DIN, NEN, ÖN, are committed to participate actively in the development of the project. Resolution 13/2006/00 taken by CEN/TC 275 on having considered the proposal for a new work item as documented in CEN/TC 275 N 319;

14 - CEN/TC 275 N 360 page 13 - * C Title Foodstuffs Determination of deoxynivalenol in cereals and cereal products - HPLC method with immunoaffinity column clean-up and UV detection * D Scope This European Standard specifies a method for the determination of deoxynivalenol in cereals (including maize) and cereal products by high performance liquid chromatography with immunoaffinity column clean-up and UV-detection. It has been validated in an inter/laboratory study on wheat flour, rice flour, oat flour, polenta, soy maize-mix, cereal based baby food and a wheat based breakfast cereal at levels from 50 µg/kg to 2000 µg/kg with a limit of quantification of 100 µg/kg and it is applicable up to 2000 µg/kg * E Deliverable EN TS TR * F Track Enquiry + Formal Vote (ENQ + FV) G Document developed in WG 5 "Biotoxins", Project leader: Susan MacDonald H Intended to be mandated No Yes; M/383/EN, items 2 and 4 I Related Directive(s) Yes; 315/1993 or 466/2001 For citation in Official Journal? not known The following CEN members, AFNOR, BSI, DIN, NEN, ÖN, are committed to participate actively in the development of the project. Resolution 14/2006/00 taken by CEN/TC 275 on having considered the proposal for a new work item as documented in CEN/TC 275 N 320; * C Title Foodstuffs Determination of zearalenone in cereals, cereal products and also cereal based food for infants and young children- HPLC method with immunoaffinity column clean up and fluorescence detection * D Scope This European Standard specifies a method for the determination of zearalenone in cereals, cereal products and also cereal products as well as foods for infants and young children by high performance liquid chromatography method with fluorescence detection and immunoaffinity column clean up. It has been validated in an inter-laboratory study with barley, maize, wheat, polenta and cereal based baby food at levels from 10 µg/kg to 335 µg /kg.

15 - CEN/TC 275 N 360 page 14 - * E Deliverable EN TS TR * F Track Enquiry + Formal Vote (ENQ + FV) G Document developed in WG 5 "Biotoxins", Project leader: Susan MacDonald H Intended to be mandated No Yes; M/383/EN, items 3 and 5 I Related Directive(s) Yes; 315/1993 or 466/2001 For citation in Official Journal? not known The following CEN members, AFNOR, BSI, DIN, NEN, ÖN, are committed to participate actively in the development of the project. Resolution 15/2006/00 taken by CEN/TC 275 on having considered the proposal for a new work item as documented in CEN/TC 275 N 332; * C Title "Foodstuffs Determination of aflatoxin B 1 in cereal based foods for infants and young children - HPLC method with immunoaffinity column clean-up * D Scope This European Standard specifies a method for the determination of aflatoxin B 1 in cereal based foods for infants and young children using high performance liquid chromatography with immunoaffinity column clean-up. The method has been validated with baby food such as naturally contaminated and spiked milk powder, i.e. infant formula at levels ranging from 0,07 µg/kg up to 0,18 µg/kg:' * E Deliverable EN TS TR * F Track Enquiry + Formal Vote (ENQ + FV) G Document developed in WG 5 "Biotoxins", Project leader: Jörg Stroka H Intended to be mandated No Yes; M/383/EN, item 6 I Related Directive(s) Yes; 315/1993 or 466/2001 For citation in Official Journal? not known The following CEN members, AFNOR, BSI, DIN, NEN, ÖN, are committed to participate actively in the development of the project.

16 - CEN/TC 275 N 360 page 15 - Resolution 16/2006/00 taken by CEN/TC 275 on having considered the proposal for a new work item as documented in CEN/TC 275 N 344; * C Title Foodstuffs Determination of ochratoxin A in cereal based foods for infants and young children - HPLC method with immunoaffinity column clean up * D Scope This European Standard specifies a method for the determination of ochratoxin A in cereal based foods for infants and young children by high performance liquid chromatography with immunoaffinity column clean up. It has been validated with cereal based food for infants and young children at levels from 0,023 µg/kg to 0,225 µg/kg. The limit of quantification of the method has been demonstrated to be 0,007 µg/kg (value derived from an in-house study). * E Deliverable EN TS TR * F Track Enquiry + Formal Vote (ENQ + FV) G Document developed in WG 5 "Biotoxins", Project leader: Pedro Burdaspal H Intended to be mandated No Yes; M/383/EN, item 7 I Related Directive(s) Yes; 315/1993 or 466/2001 For citation in Official Journal? not known The following CEN members AFNOR, BSI, DIN, NEN, ÖN, are committed to participate actively in the development of the project. Resolution 17/2006/00 taken by CEN/TC 275 on having considered the proposal for a new work item as documented in CEN/TC 275 N 323;

17 - CEN/TC 275 N 360 page 16 - * C Title Foodstuffs Determination of ochratoxin A in dried fruits - HPLC method with immunoaffinity column clean up * D Scope This European Standard specifies a method for the determination of ochratoxin A in dried fruits by high performance liquid chromatography with immunoaffinity column clean up. It has been validated in an inter-laboratory test with currants, raisins, sultanas, mixed dried fruit and dried figs at levels from 1,1 µg/kg to 11 µg/kg. * E Deliverable EN TS TR * F Track Enquiry + Formal Vote (ENQ + FV) G Document developed in WG 5 "Biotoxins", Project leader: Susan MacDonald H Intended to be mandated No Yes; M/383/EN, item 8 I Related Directive(s) Yes; 315/1993 or 466/2001 For citation in Official Journal? not known The following CEN members AFNOR, BSI, DIN, NEN, ÖN are committed to participate actively in the development of the project. Resolution 18/2006/00 taken by CEN/TC 275 on having considered the proposal for a new work item as documented in CEN/TC 275 N 324; * C Title Foodstuffs Determination of ochratoxin A in cocoa powder HPLC method with clean up on immunoaffinity column * D Scope This European Standard specifies a method for the determination of ochratoxin A content in cocoa powder by HPLC with clean up on immunoaffinity column. It has been validated in an inter-laboratory study for cocoa powder at levels ranging from 0,2 µg/kg and 1,5 µg/kg. The limit of detection was 0,05 µg/kg and the limit of quantification was 0,1 µg/kg

18 - CEN/TC 275 N 360 page 17 - * E Deliverable EN TS TR * F Track Enquiry + Formal Vote (ENQ + FV) G Document developed in WG 5 Project leader: Carlo Brera H Intended to be mandated No Yes; M/mandate number I Related Directive(s) Yes; 315/1993 or 466/2001 For citation in Official Journal? not known The following CEN members, AFNOR, BSI, DIN, NEN, ÖN, are committed to participate actively in the development of the project. Resolution 19/2006/00 taken by CEN/TC 275 on having considered the proposal for a new work item as documented in CEN/TC 275 N 325; * C Title Foodstuffs Determination of aflatoxin B 1 in maize HPLC method with clean up on immunoaffinity column * D Scope This European Standard specifies a method for the determination of aflatoxin B 1 in maize by HPLC with clean up on immunoaffinity column. It has been prevalidated in a single laboratory validation for 9 maize samples at levels ranging from 2,0 µg/kg to 17,6 µg/kg * E Deliverable EN TS TR * F Track Enquiry + Formal Vote (ENQ + FV) G Document developed in WG 5 Project leader: Carlo Brera H Intended to be mandated No Yes; M/mandate number I Related Directive(s) No Yes; 315/1993 or 466/2001 For citation in Official Journal? not known The following CEN members, AFNOR, BSI, DIN, NEN, ÖN, are committed to participate actively in the development of the project.

19 - CEN/TC 275 N 360 page 18 - Resolution 20/2006/00 taken by CEN/TC 275 on having considered the proposal for a new work item as documented in CEN/TC 275 N 334; the new edition of CEN/TR * C Title Foodstuffs Performance criteria for methods of analysis of mycotoxins * D Scope This Technical Report specifies criteria for the selection of methods of analysis for mycotoxins. Criteria covered are e.g. the repeatability, reproducibility, recovery, extraction solvents, applicability and food types. Performance criteria are included for aflatoxin B 1, total aflatoxins B 1, B 2, G 1 and G 2, aflatoxin M 1, ochratoxin A, patulin, fumonisins B 1 and B 2, deoxynivalenol, nivalenol, HT-2 toxin, T-2-toxin and zearalenone. * E Deliverable EN TS TR * F Track TC Approval (TCA) by correspondence G Document developed in WG 5 Project leader: Van Egmond H Intended to be mandated No Yes; M 383/EN, item 10 I Related Directive(s) Yes; 315/1993 or 466/2001 TC Approval (TCA) in meeting For citation in Official Journal? not known The following CEN members, AFNOR, BSI, DIN, NEN, ÖN, are committed to participate actively in the development of the project. Resolution 21/2006/00 taken by CEN/TC 275 on Subject: CEN/TC 275 Corrigenda for EN (OTA in barley and roasted coffee) and EN (OTA in wine and beer), Following the request of CEN/TC 275/WG 5, CEN/TC 275 agrees to publish corrigenda for EN (OTA in barley and roasted coffee) and EN (OTA in wine and beer) in which an incorrect equation for the determination of the concentration of the mycotoxin stock solution has been used, as outlined in N 334.

20 - CEN/TC 275 N 360 page 19 - Resolution 22/2006/00 taken by CEN/TC 275 on Subject: CEN/TC 275 Adoption of a new work item CEN/TC 275 "Food analysis horizontal methods" having considered the proposal for a new work item as documented in CEN/TC 275 N 314; confirming that the new work item falls within its scope; confirming that the new work item corresponds to real market needs; confirming that the resources to complete the work as described below are available; decides to register the work item described below in its active programme of work. * 1 This item corresponds to * 2 This adoption is based on the availability of a (pre) first draft N 314 * 3 The decision was taken * 4 Title Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Performance characteristics of molecular detection methods * 5 Scope This standard is applicable to the detection of food-borne pathogens in foodstuffs and isolates obtained from them using molecular detection methods, e.g. polymerase chain reaction (PCR). The standard describes minimum requirements of performance characteristics for the detection of nucleic acid sequences (DNA or RNA) by molecular methods. NOTE 1 The standard can also be used for other purposes in the foodstuffs sector (e.g. the detection of food-borne pathogens in environmental samples), and for animal feeding stuffs. NOTE 2 Because of the rapid progress in this field, the examples given are those most frequently used at the time when the standard was developed. * 6 Deliverable EN TS TR * 7 Track Enquiry + Formal Vote (ENQ + FV) 8 Reference Document (if any) CEN/TC 275 N 314 * 9 Document developed in WG 6/TAG 3 * 10 Intended to be mandated No Yes; M/mandate number * 11 Related Directive(s) No Yes; 882/2004, 852/2004, 853/2004, 854/2004 For citation in Official Journal? not known * 12 Commitment: The following CEN members, AFNOR, BSI, DIN, DS, NEN are committed to participate actively in the development of the project. 13 Vienna Agreement Parallel CEN Lead (decision taken by ISO) ISO Lead (decision taken by CEN) Taking over of published ISO document ISO Reference: ref. ISO ID #: ID

21 - CEN/TC 275 N 360 page 20 - * 14 Timeframe If parallel ISO lead, the work item is transferred to ISO/TC/SC/WG number. The following CEN members being committed to participate in the ISO work, names of the CEN members and the Project Leader being name of Project Leader months max. (circulation of first working draft) months max. (dispatch of draft for PQ/ENQ/UAP or TCA to CMC) months max. (dispatch of draft for FV to CMC) Note: Stage code only valid for EN deliverable Note: Tolerances up to 9 months may be applied for before stages and 45.99, providing full justification is provided and relevant delegated resolution template is used for requesting the tolerances. CEN/TC 275 adopts doc N 314 not only as first working document but also for launching the parallel Enquiry as pren ISO and asks CMC, ISO/CS and the ISO-secretariat to take the necessary steps. Resolution 23/2006/00 taken by CEN/TC 275 on Subject: CEN/TC 275 Adoption of a new work item CEN/TC 275 "Food analysis horizontal methods" having considered the proposal for a new work item as documented in CEN/TC 275 N 316; confirming that the new work item falls within its scope; confirming that the new work item corresponds to real market needs; confirming that the resources to complete the work as described below are available; decides to register the work item described below in its active programme of work. * 1 This item corresponds to * 2 This adoption is based on the availability of a (pre) first draft N 314 * 3 The decision was taken * 4 Title Microbiology of food and animal feeding stuffs Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions * 5 Scope This standard is applicable to the detection of food-borne pathogens in foodstuffs, and isolates obtained from them, using the polymerase chain reaction (PCR). The standard describes requirements for the amplification and detection of nucleic acid sequences (DNA or RNA after reverse transcription) by real-time PCR. The minimum requirements laid down in the standard provide the basis for comparable and reproducible results in individual and between different laboratories. NOTE The standard can also be used for other purposes in the foodstuffs sector (e.g. the detection of food-borne pathogens in environmental samples), and for animal feeding stuffs. NOTE Because of the rapid progress in this field, the examples given are those most frequently used at time when the standard was developed.

22 - CEN/TC 275 N 360 page 21 - * 6 Deliverable EN TS TR * 7 Track Enquiry + Formal Vote (ENQ + FV) 8 Reference Document (if any) CEN/TC 275 N 316 * 9 Document developed in WG 6/TAG 3 * 10 Intended to be mandated No Yes; M * 11 Related Directive(s) No Yes; 882/2004, 852/2004, 853/2004, 854/2004 For citation in Official Journal? not known * 12 Commitment: The following CEN members, AFNOR, BSI, DIN, DS, NEN are committed to participate actively in the development of the project. 13 Vienna Agreement * 14 Timeframe Parallel CEN Lead (decision taken by ISO) ISO Lead (decision taken by CEN) Taking over of published ISO document ISO Reference: ref. ISO ID #: ID If parallel ISO lead, the work item is transferred to ISO/TC/SC/WG number. The following CEN members being committed to participate in the ISO work, names of the CEN members and the Project Leader being name of Project Leader months max. (circulation of first working draft) months max. (dispatch of draft for PQ/ENQ/UAP or TCA to CMC) months max. (dispatch of draft for FV to CMC) Note: Stage code only valid for EN deliverable Note: Tolerances up to 9 months may be applied for before stages and 45.99, providing full justification is provided and relevant delegated resolution template is used for requesting the tolerances. CEN/TC 275 adopts doc N 316 not only as first working document but also for launching the parallel Enquiry as pren ISO and asks CMC, ISO/CS and the ISO-secretariat to take the necessary steps. Resolution 24/2006/00 taken by CEN/TC 275 on Subject: CEN/TC 275 Adoption of a new work item CEN/TC 275 "Food analysis horizontal methods" having considered the proposal for a new work item as documented in CEN/TC 275 N 342; confirming that the new work item falls within its scope; confirming that the new work item corresponds to real market needs; confirming that the resources to complete the work as described below are available; decides to register the work item described below in its active programme of work. * 1 This item corresponds to the new edition of CEN ISO/TS

23 - CEN/TC 275 N 360 page 22 - * 2 This adoption is based on the availability of a (pre) first draft N 342 * 3 The decision was taken * 4 Title Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory. * 5 Scope This European standard provides the general terminology related to quality assurance and specifies the minimum requirements for the preparation of culture media to be used for the microbiological analysis of products intended for human consumption or animal feeding. It may also be applied to culture media to be used for the microbiological analysis of all kinds of water. These requirements are applicable to four categories of culture media used in laboratories that prepare and/or use culture media for performing microbiological analyses : - commercially manufactured ready-to-use media ; - media to be remelted, supplemented and distributed; - media prepared from commercially available dehydrated formulations ; - media prepared from its individual components. * 6 Deliverable EN TS TR * 7 Track TC Approval (TCA) by correspondence 8 Reference Document (if any) CEN/TC 275 N 342 * 9 Document developed in WG 6/TAG 3, Marius Grand TC Approval (TCA) in meeting * 10 Intended to be mandated No Yes; M/mandate number * 11 Related Directive(s) No Yes; 882/2004, 852/2004, 853/2004, 854/2004 For citation in Official Journal? not known * 12 Commitment: The following CEN members, AFNOR, BSI, DIN, DS, NEN, are committed to participate actively in the development of the project. 13 Vienna Agreement * 14 Timeframe Parallel CEN Lead (decision taken by ISO) ISO Lead (decision taken by CEN) Taking over of published ISO document ISO Reference: ref. ISO ID #: ID If parallel ISO lead, the work item is transferred to ISO/TC/SC/WG number. The following CEN members being committed to participate in the ISO work, names of the CEN members and the Project Leader being name of Project Leader months max. (circulation of first working draft) months max. (dispatch of draft for PQ/ENQ/UAP or TCA to CMC) months max. (dispatch of draft for FV to CMC) Note: Stage code only valid for EN deliverable Note: Tolerances up to 9 months may be applied for before stages and 45.99, providing full justification is provided and relevant delegated resolution template is used for requesting the tolerances.

24 - CEN/TC 275 N 360 page 23 - CEN/TC 275 adopts doc N 342 not only as first working document but also for launching the parallel Formal Vote as prcen ISO/TS and asks CMC and ISO/CS and ISO-secretariat to take the necessary steps. Resolution 25/2006/00 taken by CEN/TC 275 on having considered the proposal for a new work item as documented in CEN/TC 275 N 231; * C Title Detection of Shiga toxin-producing Escherichia coli other than E. coli O157 * D Scope This document detects Shiga toxin-producing Escherichia coli other than E. coli O157 * E Deliverable EN TS TR * F Track Enquiry + Formal Vote (ENQ + FV) Primary Questionnaire + Formal Vote (PQ+FV) Unique Acceptance Procedure, 5 months (UAP) a) Shortened UAP, 3 months b) a) Also to be used for the conversion of CEN/TS into EN without updating b) To be used only in case of Amendments G Document developed in WG 6 "Microbial contaminants H Intended to be mandated No Yes; I Related Directive(s) No Yes; 882/2004, 852/2004, 853/2004, 854/2004 TC Approval (TCA) by correspondence TC Approval (TCA) in meeting For citation in Official Journal? Not known Commitment: The following CEN members, The following CEN members, AFNOR, BSI, DIN, DS, NEN are committed to participate actively in the development of the project. Resolution 26/2006/00 taken by CEN/TC 275 on having considered the proposal for a new work item as documented in CEN/TC 275 N 231;

25 - CEN/TC 275 N 360 page 24 - * C Title Microbiology of food and animal feeding stuffs Detection of food-borne viruses in food based on PCR technique * D Scope This standard describes the detection of food-borne viruses in food based on PCR technique * E Deliverable EN TS TR * F Track Enquiry + Formal Vote (ENQ + FV) G Document developed in WG 6 "Microbial contaminants" TAG 4 "Viruses" H Intended to be mandated No Yes; M/381, item I/1 I Related Directive(s) No Yes; 178/2002 For citation in Official Journal? Not known Resolution 27/2006/00 taken by CEN/TC 275 on having considered the proposal for a new work item as documented in CEN/TC 275 N 231; * C Title Microbiology of food and animal feeding stuffs Detection and quantification of histamine * D Scope This standard describes the detection and quantification of histamine * E Deliverable EN TS TR * F Track Enquiry + Formal Vote (ENQ + FV G Document developed in WG 6 "Microbial contaminants H Intended to be mandated No Yes; M/381, item I/2

26 - CEN/TC 275 N 360 page 25 - I Related Directive(s) No Yes; 2073/2005 "Commission Regulation on microbiological criteria for foodstuffs" For citation in Official Journal? Not known Resolution 28/2006/00 taken by CEN/TC 275 on having considered the proposal for a new work item as documented in CEN/TC 275 N 231; * C Title Microbiology of food and animal feeding stuffs Detection of staphylococcal enterotoxin for dairy products and other matrices * D Scope This standard describes the detection of staphylococcal enterotoxin for dairy products and other matrices * E Deliverable EN TS TR * F Track Enquiry + Formal Vote (ENQ + FV G Document developed in WG 6 "Microbial contaminants H Intended to be mandated No Yes; M/381, item I/3 I Related Directive(s) No Yes; Commission Regulation on microbiological criteria for foodstuffs 2073/2005 For citation in Official Journal? Not known Resolution 29/2006/00 taken by CEN/TC 275 on having considered the proposal for a new work item as documented in CEN/TC 275 N 231;